nuoL NADH:quinone oxidoreductase subunit L [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: nuoL
Official Full Name: NADH:quinone oxidoreductase subunit L
Primary source: ECOCYC:EG12092
Locus tag: b2278
See related: ASAP:ABE-0007532
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2272
Summary: NuoL and NuoM are located at the distal end of the membrane arm. the redox centers are in the peripheral arm. [More information is available at EcoGene: EG12092]. NuoL is part of the inner membrane component of NADH dehydrogenase I . [More information is available at EcoCyc: EG12092].
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Genomic context

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Sequence:: NC_000913.3 (2393205..2395046, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NuoL is involved in the high efficiency pumping mechanism in complex I.
Title: Roles of subunit NuoL in the proton pumping coupling mechanism of NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli.
The results do not support the hypothesis that the lateral helix of the NuoL subunit functions like a piston.
Title: Constraining the Lateral Helix of Respiratory Complex I by Cross-linking Does Not Impair Enzyme Activity or Proton Translocation.
To explore the functional relevance of the pseudosymmetric inverted-repeat structures of the antiporter-like subunits NuoL/M/N, we constructed a symmetry-related structure of a possible alternate-access state.
Title: Electrostatics, hydration, and proton transfer dynamics in the membrane domain of respiratory complex I.
Data show that the mutation of the conserved amino acid D563(L), which is part of the horizontal helix of the Escherichia coli complex I subunit NuoL, leads to a reduced H(+)/e(-) stoichiometry.
Title: Asp563 of the horizontal helix of subunit NuoL is involved in proton translocation by the respiratory complex I.
NuoL was truncated at its C- and N-termini, which resulted in low production of a fragile Complex I with negligible activity.
Title: Probing the mechanistic role of the long α-helix in subunit L of respiratory Complex I from Escherichia coli by site-directed mutagenesis.
Mutagenesis of the L, M, and N subunits of Complex I from Escherichia coli indicates a common role in function.
Title: Mutagenesis of the L, M, and N subunits of Complex I from Escherichia coli indicates a common role in function.
Data show that in L, M, and N subunits of Complex I, transmembrane helices 9 and 12 are predicted to form the discontinuous helices, which are likely to play a key role in function.
Title: The transmembrane helices of the L, M, and N subunits of Complex I from E. coli can be assigned on the basis of conservation and hydrophobic moment analysis.
the membrane subunit NuoL(ND5) is involved in the indirect proton pumping mechanism of Escherichia coli complex I
Title: The membrane subunit NuoL(ND5) is involved in the indirect proton pumping mechanism of Escherichia coli complex I.
Data show that subunits NuoL and M jointly occupy a distal half of the membrane domain of respiratory complex I, separated by about 10nm from the interface with the peripheral arm.
Title: Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I.
An antiporter related subunit of NADH dehydrogenase I produced in Saccharomyces cerevisiae functions as transporter of Na+ and K+.
Title: Transport of Na(+) and K (+) by an antiporter-related subunit from the Escherichia coli NADH dehydrogenase I produced in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
contributes_to NADH dehydrogenase (ubiquinone) activity	IDA Inferred from Direct Assay more info	PubMed
enables NADH dehydrogenase (ubiquinone) activity	IEA Inferred from Electronic Annotation more info
contributes_to NADH dehydrogenase activity	IDA Inferred from Direct Assay more info	PubMed
enables quinone binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in ATP synthesis coupled electron transport	IEA Inferred from Electronic Annotation more info
involved_in electron transport coupled proton transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in electron transport coupled proton transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in respiratory electron transport chain	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of NADH dehydrogenase complex	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of respiratory chain complex I	IBA Inferred from Biological aspect of Ancestor more info
part_of respiratory chain complex I	IDA Inferred from Direct Assay more info	PubMed
part_of respiratory chain complex I	IMP Inferred from Mutant Phenotype more info	PubMed
part_of respiratory chain complex I	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: NADH:quinone oxidoreductase subunit L

NP_416781.1

EC 7.1.1.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2393205..2395046 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416781.1 NADH:quinone oxidoreductase subunit L [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416781.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A7H9N7M2

Conserved Domains (1) summary

PRK06590
Location:1 → 601

PRK06590; NADH:ubiquinone oxidoreductase subunit L; Reviewed