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    grcA stress-induced alternate pyruvate formate-lyase subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947068, updated on 3-Dec-2024

    Summary

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    Official Symbol
    grcA
    Official Full Name
    stress-induced alternate pyruvate formate-lyase subunit
    Primary source
    ECOCYC:EG11784
    Locus tag
    b2579
    See related
    ASAP:ABE-0008489
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2577; yfiD
    Summary
    YfiD is required for MazF-mediated cell death after either DNA damage or inhibition of translation (Amitai, 2009). [More information is available at EcoGene: EG11784]. The yfiD gene encodes a glycyl radical protein that can replace an oxidatively damaged pyruvate formate-lyase subunit . [More information is available at EcoCyc: EG11784].
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    Genomic context

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    Sequence:
    NC_000913.3 (2716066..2716449, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative LysR-type DNA-binding transcriptional regulator YfiE Neighboring gene cysteine/O-acetylserine exporter EamB Neighboring gene uracil-DNA glycosylase Neighboring gene putative methyltransferase YfiF

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. HlyX, the FNR homologue of Actinobacillus pleuropneumoniae, is a [4Fe-4S]-containing oxygen-responsive transcription regulator that anaerobically activates FNR-dependent class I promoters via an enhanced AR1 contact. Green J, et al. Mol Microbiol, 1997 May. PMID 9179852
    2. Proteome analysis of metabolically engineered Escherichia coli producing Poly(3-hydroxybutyrate). Han MJ, et al. J Bacteriol, 2001 Jan. PMID 11114930, Free PMC Article
    3. Rescuing activity of oxygen-damaged pyruvate formate-lyase by a spare part protein. Andorfer MC, et al. J Biol Chem, 2021 Dec. PMID 34801558, Free PMC Article
    4. Downregulation of Escherichia coli yfiD expression by FNR occupying a site at -93.5 involves the AR1-containing face of FNR. Green J, et al. Mol Microbiol, 1998 Aug. PMID 9767578
    5. A novel promoter architecture for microaerobic activation by the anaerobic transcription factor FNR. Marshall FA, et al. Mol Microbiol, 2001 Feb. PMID 11169114

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Rescuing activity of oxygen-damaged pyruvate formate-lyase by a spare part protein.
      Title: Rescuing activity of oxygen-damaged pyruvate formate-lyase by a spare part protein.
    2. N-terminus verified by Edman degradation on complete protein
      Title: Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables formate C-acetyltransferase activity EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within response to stress IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    stress-induced alternate pyruvate formate-lyase subunit

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2716066..2716449 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417074.1 stress-induced alternate pyruvate formate-lyase subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417074.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A192CAF1, A0AA36KZE6, Q1R8F3
      Conserved Domains (1) summary
      PRK11127
      Location:1 → 127
      PRK11127; autonomous glycyl radical cofactor GrcA; Provisional
    Nucleotide Protein
    Heading Accession and Version

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