rpoH RNA polymerase sigma factor RpoH [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: rpoH
Official Full Name: RNA polymerase sigma factor RpoH
Primary source: ECOCYC:EG10897
Locus tag: b3461
See related: ASAP:ABE-0011303
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3445; fam; hin; htpR
Summary: RpoH is phosphorylated at Tyr-260, in HTH DNA-binding region 4.2, attenuating activity. de-phosphorylated by Etp. rpoH can be deleted, but null mutants do not grow above 20C (Zhou, 1988). [More information is available at EcoGene: EG10897]. Sigma 32 is the primary sigma factor controlling the heat shock response during log-phase growth. [More information is available at EcoCyc: EG10897].
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Genomic context

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Sequence:: NC_000913.3 (3599929..3600783, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Escherichia coli small heat shock protein IbpA plays a role in regulating the heat shock response by controlling the translation of sigma[32].
Title: Escherichia coli small heat shock protein IbpA plays a role in regulating the heat shock response by controlling the translation of σ(32).
Data report that mistranslation in Escherichia coli increase the protein level of the heat shock sigma factor RpoH and protect cells against heat stress. Surprisingly, this increase in RpoH due to mistranslation is dependent on the presence of the general stress response sigma factor RpoS.
Title: Increased mistranslation protects E. coli from protein misfolding stress due to activation of a RpoS-dependent heat shock response.
The increased synthesis of sigma(32) is accompanied by stabilization of sigma(32), which is normally very unstable and rapidly degraded by the membrane-localized FtsH protease. It was recently found that sigma(32) must be localized to the inner membrane by the SRP-dependent pathway to work properly for regulation, but the roles played by membrane and other components of the cell remained unknown.
Title: Regulation of the heat shock response in Escherichia coli: history and perspectives.
BAH1, an E3 ligase from plant that has a similar zinc finger domain to DnaJ, can perform block the effect of DnaK on sigma(32) in Escherichia coli.
Title: BAH1 an E3 Ligase from Arabidopsis thaliana Stabilizes Heat Shock Factor σ(32) of Escherichia coli by Interacting with DnaK/DnaJ Chaperone Team.
Data indicate that the sigma factor 32 (sigma32)-Ffh (the protein subunit of signal recognition particle (SRP) interaction was significantly affected by mutations in this region that compromise the feedback regulation.
Title: A Novel SRP Recognition Sequence in the Homeostatic Control Region of Heat Shock Transcription Factor σ32.
The interaction of DnaK with DnaJ, GrpE, or sigma32 becomes weaker when DnaK is glutathionylated during oxidative stress, and the interaction is restored upon deglutathionylation.
Title: Glutathionylation of the Bacterial Hsp70 Chaperone DnaK Provides a Link between Oxidative Stress and the Heat Shock Response.
Data suggest that neither DnaK nor GroEL singly can modulate sigma32 stability in vivo; there is ordered network between them, where GroEL acts upstream of DnaK.
Title: GroEL to DnaK chaperone network behind the stability modulation of σ(32) at physiological temperature in Escherichia coli.
ThiS, an ubiquitin-like protein, is covalently attached to delta(32) and at least 27 other proteins, leading to their subsequent degradation by proteases, in a similar manner to the ubiquitin-proteasome system (UPS) in eukaryotes.
Title: Heat shock transcription factor δ³² is targeted for degradation via an ubiquitin-like protein ThiS in Escherichia coli.
co-opts the signal recognition particle to regulate protein homeostasis
Title: Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli.
DnaJ-promoted binding of DnaK to multiple sites on sigma32 in the presence of ATP.
Title: DnaJ-promoted binding of DnaK to multiple sites on σ32 in the presence of ATP.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity	IDA Inferred from Direct Assay more info	PubMed
enables bacterial-type RNA polymerase core enzyme binding	IDA Inferred from Direct Assay more info	PubMed
enables core promoter sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sigma factor activity	IDA Inferred from Direct Assay more info	PubMed
enables sigma factor activity	IEA Inferred from Electronic Annotation more info
enables site-specific recombinase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA integration	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA-templated transcription	ISM Inferred from Sequence Model more info	PubMed
involved_in DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated transcription initiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription initiation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in response to heat	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to heat	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to heat	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of cytosolic DNA-directed RNA polymerase complex	ISS Inferred from Sequence or Structural Similarity more info
part_of invertasome	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: RNA polymerase sigma factor RpoH

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3599929..3600783 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417918.1 RNA polymerase sigma factor RpoH [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417918.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5B9AGI9

Conserved Domains (1) summary

PRK06596
Location:1 → 284

PRK06596; RNA polymerase factor sigma-32; Reviewed