yidC membrane protein insertase YidC [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: yidC
Official Full Name: membrane protein insertase YidC
Primary source: ECOCYC:EG11197
Locus tag: b3705
See related: ASAP:ABE-0012120
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3698
Summary: yidC is an essential gene. [More information is available at EcoGene: EG11197]. YidC mediates membrane insertion/assembly of inner membrane proteins . [More information is available at EcoCyc: EG11197].
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Genomic context

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Sequence:: NC_000913.3 (3885076..3886722)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Monitoring the binding and insertion of a single transmembrane protein by an insertase.
Title: Monitoring the binding and insertion of a single transmembrane protein by an insertase.
The Escherichia coli Outer Membrane beta-Barrel Assembly Machinery (BAM) Crosstalks with the Divisome.
Title: The Escherichia coli Outer Membrane β-Barrel Assembly Machinery (BAM) Crosstalks with the Divisome.
Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG Translocase.
Title: Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG Translocase.
Both PpiD and YidC contacted the lateral gate, the plug domain, and the periplasmic cavity of SecY.
Title: Noncompetitive binding of PpiD and YidC to the SecYEG translocon expands the global view on the SecYEG interactome in Escherichia coli.
In addition to providing virtual insights about how YidC inserts single-spanning membrane proteins, our study also demonstrates a valuable in vivo tracking method that can be applied to study more complicated substrates or other translocases.
Title: Tracking the Stepwise Movement of a Membrane-inserting Protein In Vivo.
YidC rapidly and efficiently binds to substrate proteins and leads to their integration into the liposome bilayer
Title: YidC-mediated membrane insertion.
The crystal structure of membrane transport protein YidC (YidC) and molecular dynamics simulation indicate that the intrinsic flexible C2 loop covered the positively charged cavity.
Title: 2.8-Å crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop.
We suggest that EH1 serves as a mechanical lever, facilitating a coordinated movement of YidC TMDs to trigger the release of nascent chains into the membrane.
Title: Structural Dynamics of the YidC:Ribosome Complex during Membrane Protein Biogenesis.
The YidC prevents LacY from misfolding by stabilizing the unfolded state from which LacY inserts structural segments stepwise into the membrane until folding is completed. During stepwise insertion, YidC and the membrane together stabilize the transient folds.
Title: YidC assists the stepwise and stochastic folding of membrane proteins.
Deletions in loop C1 affected the activity of YidC but did not influence ribosome binding or substrate insertion, whereas loop C2 was involved in ribosome binding. Combining the deletion with removal of the C terminus abolished YidC-mediated insertion.
Title: Role of the Cytosolic Loop C2 and the C Terminus of YidC in Ribosome Binding and Insertion Activity.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables membrane insertase activity	IBA Inferred from Biological aspect of Ancestor more info
enables membrane insertase activity	IDA Inferred from Direct Assay more info	PubMed
enables membrane insertase activity	IEA Inferred from Electronic Annotation more info
enables membrane insertase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in protein folding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein insertion into membrane	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein insertion into membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in protein insertion into membrane from inner side	IDA Inferred from Direct Assay more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in protein transport by the Sec complex	IDA Inferred from Direct Assay more info	PubMed
involved_in protein-containing complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of cell envelope Sec protein transport complex	IDA Inferred from Direct Assay more info	PubMed
part_of cell envelope Sec protein transport complex	IPI Inferred from Physical Interaction more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	NAS Non-traceable Author Statement more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	RCA inferred from Reviewed Computational Analysis more info	PubMed

General protein information

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Preferred Names: membrane protein insertase YidC

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3885076..3886722

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418161.1 membrane protein insertase YidC [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418161.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A8S7ELE2

Conserved Domains (1) summary

PRK01318
Location:1 → 548

PRK01318; membrane protein insertase; Provisional