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    nagC DNA-binding transcriptional dual regulator NagC [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945285, updated on 3-Dec-2024

    Summary

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    Official Symbol
    nagC
    Official Full Name
    DNA-binding transcriptional dual regulator NagC
    Primary source
    ECOCYC:EG10636
    Locus tag
    b0676
    See related
    ASAP:ABE-0002299
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0664; nagR
    Summary
    The NagC, "N-acetylglucosamine," transcriptional dual regulator participates in regulating the phosphotransferase system (PTS) . [More information is available at EcoCyc: EG10636].
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    Genomic context

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    Sequence:
    NC_000913.3 (700374..701594, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene asparagine synthetase B Neighboring gene UMP phosphatase Neighboring gene N-acetylglucosamine-6-phosphate deacetylase Neighboring gene glucosamine-6-phosphate deaminase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Regulation of PTS gene expression by the homologous transcriptional regulators, Mlc and NagC, in Escherichia coli (or how two similar repressors can behave differently). Plumbridge J. J Mol Microbiol Biotechnol, 2001 Jul. PMID 11361067
    2. A dominant mutation in the gene for the Nag repressor of Escherichia coli that renders the nag regulon uninducible. Plumbridge JA. J Gen Microbiol, 1992 May. PMID 1645125
    3. Complex synergistic amino acid-nucleotide interactions contribute to the specificity of NagC operator recognition and induction. Fernandez M, et al. Microbiology (Reading), 2019 Jul. PMID 31107208
    4. Operator recognition by the ROK transcription factor family members, NagC and Mlc. Bréchemier-Baey D, et al. Nucleic Acids Res, 2015 Jan. PMID 25452338, Free PMC Article
    5. High-level recombinant protein production by overexpression of Mlc in Escherichia coli. Cho S, et al. J Biotechnol, 2005 Sep 23. PMID 15916829

    See all (40) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The NagC mutants exhibiting this paradoxical behaviour have basic amino acids, arginine or lysine, at two critical positions of the recognition helix.
      Title: Complex synergistic amino acid-nucleotide interactions contribute to the specificity of NagC operator recognition and induction.
    2. This work identifies four amino acids in the linker region which allow NagC to distinguish between a C/G and an A/T bp at positions +/-11 of the operators.
      Title: Operator recognition by the ROK transcription factor family members, NagC and Mlc.
    3. NagC binds to a single, high-affinity site overlapping the promoter of galP.
      Title: Repression of galP, the galactose transporter in Escherichia coli, requires the specific regulator of N-acetylglucosamine metabolism.
    4. Results show that changes at either of two Mlc operators upstream of ptsG are sufficient to lose regulation by Mlc but that both types of changes in both operators are necessary to convert ptsG to a gene regulated by NagC.
      Title: Switching control of expression of ptsG from the Mlc regulon to the NagC regulon.
    5. Mutations in NagC, at positions which form a structural motif resembling a glucose binding site in Mlc, produce permanently repressing forms of NagC, suggesting that this motif forms a N-acetylglucosamine 6-phosphate binding site in NagC.
      Title: Different regions of Mlc and NagC, homologous transcriptional repressors controlling expression of the glucose and N-acetylglucosamine phosphotransferase systems in Escherichia coli, are required for inducer signal recognition.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of DNA-templated transcription IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-binding transcriptional dual regulator NagC

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      700374..701594 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415202.1 DNA-binding transcriptional dual regulator NagC [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415202.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6L7CAG1
      Conserved Domains (2) summary
      pfam00480
      Location:89 → 392
      ROK; ROK family
      cl21459
      Location:25 → 77
      HTH; Helix-turn-helix domains
    Nucleotide Protein
    Heading Accession and Version

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