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    pykF pyruvate kinase 1 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946179, updated on 3-Dec-2024

    Summary

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    Official Symbol
    pykF
    Official Full Name
    pyruvate kinase 1
    Primary source
    ECOCYC:EG10804
    Locus tag
    b1676
    See related
    ASAP:ABE-0005600
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1672
    Summary
    Pyruvate kinase I and pyruvate kinase II differ in physical and chemical properties as well as in their kinetic behavior. [More information is available at EcoCyc: EG10804].
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    Genomic context

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    Sequence:
    NC_000913.3 (1755698..1757110)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene fumarase D Neighboring gene protein YnhH Neighboring gene murein lipoprotein Neighboring gene L,D-transpeptidase LdtE

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Direct genomic sequencing of bacterial DNA: the pyruvate kinase I gene of Escherichia coli. Ohara O, et al. Proc Natl Acad Sci U S A, 1989 Sep. PMID 2674937, Free PMC Article
    2. Crystal structure of Escherichia coli pyruvate kinase type I: molecular basis of the allosteric transition. Mattevi A, et al. Structure, 1995 Jul 15. PMID 8591049
    3. Analysis of progress curves. Rate law of pyruvate kinase type I from Escherichia coli. Markus M, et al. Biochem J, 1980 Sep 1. PMID 7011316, Free PMC Article
    4. AMP- and fructose 1,6-bisphosphate-activated pyruvate kinases from Escherichia coli. Malcovati M, et al. Methods Enzymol, 1982. PMID 6759852
    5. The control of pyruvate kinases of Escherichia coli. I. Physicochemical and regulatory properties of the enzyme activated by fructose 1,6-diphosphate. Waygood EB, et al. J Biol Chem, 1974 Jan 10. PMID 4588693

    See all (59) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. data are consistent with the domain rotation model but inconsistent with the rigid body reorientation model given the increased flexibility at the interdomain interface, and we can for the first time explain how fructose 1,6-bisphosphate affects the active site.
      Title: Conformational Dynamics and Allostery in Pyruvate Kinase.
    2. The elevated cAMP in PB25 suggests that pyruvate kinase may participate in glucose catabolite repression by serving among all of the factors that tighten gene expression.
      Title: Pyruvate kinase-deficient Escherichia coli exhibits increased plasmid copy number and cyclic AMP levels.
    3. N-terminus verified by Edman degradation on complete protein
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    4. A pykF-gene knockout mutation effects gene expression and metabolic fluxes in Escherichia coli.
      Title: Effect of a pyruvate kinase (pykF-gene) knockout mutation on the control of gene expression and metabolic fluxes in Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables potassium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables pyruvate kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables pyruvate kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pyruvate kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within glycolytic process EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in glycolytic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in glycolytic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to heat IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 
    part_of pyruvate kinase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    pyruvate kinase 1
    NP_416191.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1755698..1757110
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416191.1 pyruvate kinase 1 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416191.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376WY06
      Conserved Domains (1) summary
      PRK09206
      Location:1 → 470
      PRK09206; pyruvate kinase PykF
    Nucleotide Protein
    Heading Accession and Version

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