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    vsr DNA mismatch endonuclease Vsr [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946476, updated on 17-Dec-2024

    Summary

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    Official Symbol
    vsr
    Official Full Name
    DNA mismatch endonuclease Vsr
    Primary source
    ECOCYC:EG11068
    Locus tag
    b1960
    See related
    ASAP:ABE-0006507
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1958
    Summary
    Mismatch repair minimizes the effects of C methylation. [More information is available at EcoGene: EG11068]. Vsr is an endonuclease that specifically recognizes and exhibits strand-specific nicking at T:G DNA mismatches, which arise from spontaneous deamination at 5-methylcytosine, the product of Dcm (cytosine) methylation . [More information is available at EcoCyc: EG11068].
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    Genomic context

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    Sequence:
    NC_000913.3 (2030448..2030918, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YodC Neighboring gene DUF808 domain-containing inner membrane protein YedI Neighboring gene putative transporter YedA Neighboring gene DNA-cytosine methyltransferase Neighboring gene putative HD superfamily phosphohydrolase YedJ Neighboring gene cell division protein DrpB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Fast quantitative assay of sequence-specific endonuclease activity based on DNA sequencer technology. Gläsner W, et al. Biol Chem Hoppe Seyler, 1992 Dec. PMID 1337970
    2. Influence of very short patch mismatch repair on SOS inducing lesions after aminoglycoside treatment in Escherichia coli. Baharoglu Z, et al. Res Microbiol, 2014 Jul-Aug. PMID 24946128
    3. CA repeat polymorphism at the D5S82 locus, proximal to adenomatous polyposis coli (APC). Breukel C, et al. Nucleic Acids Res, 1991 Oct 25. PMID 1945867, Free PMC Article
    4. Levels of the Vsr endonuclease do not regulate stationary-phase reversion of a Lac- frameshift allele in Escherichia coli. Foster PL, et al. J Bacteriol, 1998 Apr. PMID 9537396, Free PMC Article
    5. The E. coli Vsr endonuclease. Assaying activity in vitro and in vivo. Cupples CG, et al. Methods Mol Biol, 2000. PMID 10957969

    See all (51) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Authors propose that lesions formed upon aminoglycoside treatment are preferentially repaired by Vsr without SOS induction in Escherichia coli and by mismatch repair when very short patch mismatch repair is impaired.
      Title: Influence of very short patch mismatch repair on SOS inducing lesions after aminoglycoside treatment in Escherichia coli.
    2. unusual patterns of base pair stability in the Dcm target sequence can explain how the Vsr endonuclease specifically recognizes the mismatched Dcm target sequence and intercalates into the DNA
      Title: Base pair opening kinetics and dynamics in the DNA duplexes that specifically recognized by very short patch repair protein (Vsr).
    3. Results suggest a mobile MutS-MutL complex in DNA mismatch repair signalling, that leaves the DNA mismatch prior to, or at the time of, activation of downstream effector molecules such as Vsr.
      Title: Physical and functional interactions between Escherichia coli MutL and the Vsr repair endonuclease.
    4. MutL recruits the Vsr and MutH to sites of DNA damage.
      Title: The Escherichia coli mismatch repair protein MutL recruits the Vsr and MutH endonucleases in response to DNA damage.
    5. N-terminus verified by Edman degradation on mature peptide
      Title: Crystallographic and functional studies of very short patch repair endonuclease.
    6. Vsr carries out a hydrolytic reaction with inversion of stereochemistry at phosphorus, compatible with an in-line attack of water and a pentacovalent transition state with trigonal bipyramidal geometry
      Title: Mechanism of the Escherichia coli DNA T:G-mismatch endonuclease (Vsr protein) probed with thiophosphate-containing oligodeoxynucleotides.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables T/G mismatch-specific endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within mismatch repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in mismatch repair IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    DNA mismatch endonuclease Vsr

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2030448..2030918 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416469.1 DNA mismatch endonuclease Vsr [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416469.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376M6I2
      Conserved Domains (1) summary
      COG3727
      Location:1 → 152
      Vsr; G:T-mismatch repair DNA endonuclease, very short patch repair protein [Replication, recombination and repair]
    Nucleotide Protein
    Heading Accession and Version

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