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    bamE outer membrane protein assembly factor BamE [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945583, updated on 3-Dec-2024

    Summary

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    Official Symbol
    bamE
    Official Full Name
    outer membrane protein assembly factor BamE
    Primary source
    ECOCYC:EG10952
    Locus tag
    b2617
    See related
    ASAP:ABE-0008614
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2613; smpA; smqA
    Summary
    smpA mutants are sensitive to rifampin, cholate, and SDS/EDTA, indicating an OM defect (Sklar, 2007). smpA bamB double mutant is non-viable (Sklar, 2007). [More information is available at EcoGene: EG10952]. SmpA is, based on sequence analysis, a lipoprotein in the outer membrane of Escherichia coli . [More information is available at EcoCyc: EG10952].
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    Genomic context

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    Sequence:
    NC_000913.3 (2753605..2753946)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene NAD kinase Neighboring gene DNA repair protein RecN Neighboring gene putative component of the Rsx system Neighboring gene ribosome association toxin RatA Neighboring gene SsrA-binding protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structure and function of BamE within the outer membrane and the β-barrel assembly machine. Knowles TJ, et al. EMBO Rep, 2011 Feb. PMID 21212804, Free PMC Article
    2. The Synthetic Phenotype of ΔbamB ΔbamE Double Mutants Results from a Lethal Jamming of the Bam Complex by the Lipoprotein RcsF. Hart EM, et al. mBio, 2019 May 21. PMID 31113901, Free PMC Article
    3. Improper Coordination of BamA and BamD Results in Bam Complex Jamming by a Lipoprotein Substrate. Tata M, et al. mBio, 2019 May 21. PMID 31113900, Free PMC Article
    4. A minimum functional form of the Escherichia coli BAM complex constituted by BamADE assembles outer membrane proteins in vitro. Wang Z, et al. J Biol Chem, 2024 Jun. PMID 38677515, Free PMC Article
    5. Identification of a chromosomally encoded ABC-transport system with which the staphylococcal erythromycin exporter MsrA may interact. Ross JI, et al. Gene, 1995 Feb 3. PMID 7883194

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A minimum functional form of the Escherichia coli BAM complex constituted by BamADE assembles outer membrane proteins in vitro.
      Title: A minimum functional form of the Escherichia coli BAM complex constituted by BamADE assembles outer membrane proteins in vitro.
    2. The authors show that cells lacking BamB and BamE have a global outer membrane protein defect that is a result of a lethal obstruction of an assembly-competent Bam complex by the lipoprotein RcsF.
      Title: The Synthetic Phenotype of ΔbamB ΔbamE Double Mutants Results from a Lethal Jamming of the Bam Complex by the Lipoprotein RcsF.
    3. The role of BamE is to promote proper coordination of RcsF-bound BamA with BamD to complete outer membrane protein assembly around RcsF.
      Title: Improper Coordination of BamA and BamD Results in Bam Complex Jamming by a Lipoprotein Substrate.
    4. Taken together, these findings suggest that BamE modulates the conformation of BamA, likely through its interactions with BamD.
      Title: BamE modulates the Escherichia coli beta-barrel assembly machine component BamA.
    5. Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli.
      Title: Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables cardiolipin binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein-macromolecule adaptor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein-macromolecule adaptor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein-macromolecule adaptor activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Gram-negative-bacterium-type cell outer membrane assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within Gram-negative-bacterium-type cell outer membrane assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in Gram-negative-bacterium-type cell outer membrane assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in Gram-negative-bacterium-type cell outer membrane assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within Gram-negative-bacterium-type cell outer membrane assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein insertion into membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within protein insertion into membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein insertion into membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein insertion into membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within protein insertion into membrane IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to antibiotic IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of Bam protein complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of Bam protein complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of Bam protein complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    outer membrane protein assembly factor BamE

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2753605..2753946
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417107.2 outer membrane protein assembly factor BamE [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417107.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AQH5
      Conserved Domains (1) summary
      PRK11548
      Location:1 → 113
      PRK11548; outer membrane protein assembly factor BamE
    Nucleotide Protein
    Heading Accession and Version

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