U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    recJ ssDNA-specific exonuclease RecJ [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947367, updated on 17-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    recJ
    Official Full Name
    ssDNA-specific exonuclease RecJ
    Primary source
    ECOCYC:EG10830
    Locus tag
    b2892
    See related
    ASAP:ABE-0009492
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2887
    Summary
    Lovett and Kolodner (1989) N-terminally sequenced RecJ, but the native RBS was not used to direct translation, so it has been removed from the Verified Set. [More information is available at EcoGene: EG10830]. Three essential activities for the initiation of recombination in the recB1080 mutant are provided by different proteins: helicase activity by RecB1080CD, 5' --> 3' exonuclease by RecJ, and RecA-single-stranded DNA filament formation by RecFOR. [More information is available at EcoCyc: EG10830].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (3036373..3038106, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene lysine--tRNA ligase Neighboring gene peptide chain release factor RF2 Neighboring gene protein disulfide isomerase DsbC Neighboring gene site-specific recombinase

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Role of the recJ gene product in UV-induced illegitimate recombination at the hotspot. Ukita T, et al. J Bacteriol, 1996 Apr. PMID 8636039, Free PMC Article
    2. RecFOR function is required for DNA repair and recombination in a RecA loading-deficient recB mutant of Escherichia coli. Ivancić-Baće I, et al. Genetics, 2003 Feb. PMID 12618388, Free PMC Article
    3. Genetic analysis of the recJ gene of Escherichia coli K-12. Lovett ST, et al. J Bacteriol, 1984 Jan. PMID 6317649, Free PMC Article
    4. The crystal structure of exonuclease RecJ bound to Mn2+ ion suggests how its characteristic motifs are involved in exonuclease activity. Yamagata A, et al. Proc Natl Acad Sci U S A, 2002 Apr 30. PMID 11972066, Free PMC Article
    5. RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination. Morimatsu K, et al. Proc Natl Acad Sci U S A, 2014 Dec 2. PMID 25411316, Free PMC Article

    See all (57) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. UV-irradiated Escherichia coli cells with a recN mutation did not exhibit increased nucleoid condensation, but chromosome replication and cytokinesis occurred. In the uvrA and recJ strains, the condensation step was delayed compared to the wild-type strain, and chromosome replication and cytokinesis did not occur.
      Title: Influence of uvrA, recJ and recN gene mutations on nucleoid reorganization in UV-treated Escherichia coli cells.
    2. Data indicate that complementary action by RecQ helicase and RecJ nuclease permits initiation of recombinational repair from all double-stranded (dsDNA) ends.
      Title: RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination.
    3. Study hypothesized that RecJ and XonA blunt the double-strand end (created by the replication fork reversal)in order to produce SOS(Con) expression.
      Title: RecA4142 causes SOS constitutive expression by loading onto reversed replication forks in Escherichia coli K-12.
    4. RecJ is needed to prepare ssDNA for subsequent loading of RecA protein. It implies that an additional type of RecA loading could exist in the cell
      Title: RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli.
    5. Data show that UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR at forks blocked in the rep mutant of Escherichia coli.
      Title: UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli.
    6. Data show that the RecJ nuclease and RecQ helicase, which partially degrade the nascent DNA at blocked replication forks, are required for the rapid recovery of DNA synthesis and prevent the potentially mutagenic bypass of UV lesions.
      Title: Nascent DNA processing by RecJ favors lesion repair over translesion synthesis at arrested replication forks in Escherichia coli.
    7. genetic analysis of recombination in a recB recD double mutant was performed; results show that conjugational recombination and DNA repair in this mutant are highly dependent on recJ, partially dependent on recFOR, and independent of recQ
      Title: Effects of recJ, recQ, and recFOR mutations on recombination in nuclease-deficient recB recD double mutants of Escherichia coli.
    8. Prebinding of single-stranded DNA in vitro by single-stranded DNA-binding protein does not inhibit RecJ degradation but, rather, enhances the activity of RecJ protein.
      Title: RecJ exonuclease: substrates, products and interaction with SSB.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 5'-3' exonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables exonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded DNA 5'-3' DNA exonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables single-stranded DNA 5'-3' DNA exonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA recombination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to X-ray IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    ssDNA-specific exonuclease RecJ
    NP_417368.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3036373..3038106 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417368.1 ssDNA-specific exonuclease RecJ [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417368.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7L7EH15, A0A8S7EIK8
      Conserved Domains (1) summary
      PRK11070
      Location:3 → 577
      PRK11070; single-stranded-DNA-specific exonuclease RecJ
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.