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    tnaC tnaAB operon leader peptide [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948223, updated on 3-Dec-2024

    Summary

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    Official Symbol
    tnaC
    Official Full Name
    tnaAB operon leader peptide
    Primary source
    ECOCYC:EG11276
    Locus tag
    b3707
    See related
    ASAP:ABE-0012128
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3700; tnaL
    Summary
    The TnaC leader peptide is required for regulation by attenuation of the tnaCAB operon. [More information is available at EcoCyc: EG11276].
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    Genomic context

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    Sequence:
    NC_000913.3 (3888435..3888509)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene membrane protein insertase YidC Neighboring gene 5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit Neighboring gene tryptophanase Neighboring gene tryptophan:H(+) symporter TnaB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Evidence suggesting cis action by the TnaC leader peptide in regulating transcription attenuation in the tryptophanase operon of Escherichia coli. Gish K, et al. J Bacteriol, 1995 Dec. PMID 8522534, Free PMC Article
    2. The regulatory TnaC nascent peptide preferentially inhibits release factor 2-mediated hydrolysis of peptidyl-tRNA. Emmanuel JS, et al. J Biol Chem, 2019 Dec 13. PMID 31712310, Free PMC Article
    3. Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan. Martínez AK, et al. Nucleic Acids Res, 2014 Jan. PMID 24137004, Free PMC Article
    4. Structural insight into nascent polypeptide chain-mediated translational stalling. Seidelt B, et al. Science, 2009 Dec 4. PMID 19933110, Free PMC Article
    5. Reproducing tna operon regulation in vitro in an S-30 system. Tryptophan induction inhibits cleavage of TnaC peptidyl-tRNA. Gong F, et al. J Biol Chem, 2001 Jan 19. PMID 11050101

    See all (35) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural basis of l-tryptophan-dependent inhibition of release factor 2 by the TnaC arrest peptide.
      Title: Structural basis of l-tryptophan-dependent inhibition of release factor 2 by the TnaC arrest peptide.
    2. Structural basis for the tryptophan sensitivity of TnaC-mediated ribosome stalling.
      Title: Structural basis for the tryptophan sensitivity of TnaC-mediated ribosome stalling.
    3. The tnaC regulatory gene from the tna operon of Escherichia coli controls the transcription of its own operon through an attenuation mechanism relying on the accumulation of arrested ribosomes during inhibition of its own translation termination.
      Title: The regulatory TnaC nascent peptide preferentially inhibits release factor 2-mediated hydrolysis of peptidyl-tRNA.
    4. The findings suggest that interactions between TnaC residue I19 and 23S rRNA nucleotide A2058 contribute to the creation of a regulatory L-Trp binding site within the translating ribosome.
      Title: Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan.
    5. determinatin of 5.8 angstrom-resolution cryo-electron microscopy & single-particle reconstruction of a ribosome stalled during translation of tnaC leader gene; nascent chain was extended within the exit tunnel, making contacts with ribosomal components
      Title: Structural insight into nascent polypeptide chain-mediated translational stalling.
    Submit: New GeneRIF Correction

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables rRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in positive regulation of tryptophan metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transcriptional attenuation by ribosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in transcriptional attenuation by ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transcriptional attenuation by ribosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tryptophan catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    tnaAB operon leader peptide

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3888435..3888509
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418163.1 tnaAB operon leader peptide [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418163.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P0AD89
      Conserved Domains (1) summary
      cl27886
      Location:1 → 24
      Tna_leader; Tryptophanase operon leader peptide
    Nucleotide Protein
    Heading Accession and Version

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