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    ilvA threonine deaminase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948287, updated on 17-Dec-2024

    Summary

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    Official Symbol
    ilvA
    Official Full Name
    threonine deaminase
    Primary source
    ECOCYC:EG10493
    Locus tag
    b3772
    See related
    ASAP:ABE-0012321
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3764; ile
    Summary
    N-terminal sequence of cloned IlvA shows fMet is clipped, but natural RBS was not used (Eisenstein, 1991) so it is not Verified, but the ATG start is reliable based on comparisons to Salmonella. [More information is available at EcoGene: EG10493]. Threonine deaminase (IlvA) carries out the first step in the synthesis of isoleucine. [More information is available at EcoCyc: EG10493].
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    Genomic context

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    Sequence:
    NC_000913.3 (3955331..3956875)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene branched-chain-amino-acid aminotransferase Neighboring gene dihydroxy-acid dehydratase Neighboring gene DNA-binding transcriptional dual regulator IlvY Neighboring gene ketol-acid reductoisomerase (NADP(+))

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Threonine deamination in Escherichia coli. I. D- and L-threonine deaminase activities of cell-free extracts. UMBARGER HE, et al. J Bacteriol, 1956 Apr. PMID 13319259, Free PMC Article
    2. Isoleucine and valine metabolism in Escherichia coli. XIX. Inhibition of isoleucine biosynthesis by glycyl-leucine. Vonder Haar RA, et al. J Bacteriol, 1972 Oct. PMID 4562390, Free PMC Article
    3. Threonine deaminase from Escherichia coli. I. Purification and properties. Calhoun DH, et al. J Biol Chem, 1973 May 25. PMID 4573981
    4. Purification of threonine deaminase from Escherichia coli. Grimminger H, et al. FEBS Lett, 1973 Sep 15. PMID 4582943
    5. Complementation between different mutations in the ilvA gene of Escherichia coli K-12. Favre R, et al. J Bacteriol, 1974 Sep. PMID 4604254, Free PMC Article

    See all (50) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The residues R362, E442, G445, A446, Y369, I460, and S461 only interact with isoleucine while E347, G350, and F352 are involved not only in the isoleucine binding but also in the valine binding.
      Title: Study and reengineering of the binding sites and allosteric regulation of biosynthetic threonine deaminase by isoleucine and valine in Escherichia coli.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables amino acid binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pyridoxal phosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pyridoxal phosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables threonine deaminase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables threonine deaminase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables threonine deaminase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within branched-chain amino acid biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in isoleucine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within isoleucine biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in isoleucine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within isoleucine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in threonine metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    threonine deaminase
    NP_418220.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3955331..3956875
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418220.1 threonine deaminase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418220.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A0B1BMI0, A0A140ND38, A0A8T6A3J9
      Conserved Domains (1) summary
      PRK09224
      Location:13 → 513
      PRK09224; threonine ammonia-lyase IlvA
    Nucleotide Protein
    Heading Accession and Version

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