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    cpxA sensor histidine kinase CpxA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948405, updated on 3-Dec-2024

    Summary

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    Official Symbol
    cpxA
    Official Full Name
    sensor histidine kinase CpxA
    Primary source
    ECOCYC:EG10163
    Locus tag
    b3911
    See related
    ASAP:ABE-0012772
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ecf; ecfB; ECK3904; eup; rssE; ssd
    Summary
    The sensor protein CpxA is part of the two-component CpxA/CpxR signal transduction system. [More information is available at EcoCyc: EG10163].
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    Genomic context

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    Sequence:
    NC_000913.3 (4103602..4104975, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 2-dehydro-3-deoxy-D-gluconate:H(+) symporter Neighboring gene 6-hydroxyaminopurine reductase Neighboring gene DNA-binding transcriptional dual regulator CpxR Neighboring gene periplasmic protein CpxP Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The sensor of the bacterial histidine kinase CpxA is a novel dimer of extracytoplasmic Per-ARNT-Sim domains. Cho THS, et al. J Biol Chem, 2024 May. PMID 38582452, Free PMC Article
    2. Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli. Thorbjarnardóttir SH, et al. Mol Gen Genet, 1978 Apr 25. PMID 353502
    3. The Cpx proteins of Escherichia coli K-12: evidence that cpxA, ecfB, ssd, and eup mutations all identify the same gene. Rainwater S, et al. J Bacteriol, 1990 May. PMID 2185221, Free PMC Article
    4. The Cpx proteins of Escherichia coli K12. Immunologic detection of the chromosomal cpxA gene product. Albin R, et al. J Biol Chem, 1986 Apr 5. PMID 3007473
    5. Evidence for a protonmotive force related regulatory system in Escherichia coli and its effects on lactose transport. Plate CA, et al. Biochemistry, 1986 Oct 7. PMID 3539185

    See all (93) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The CpxRA two-component system of adherent and invasive Escherichia coli contributes to epithelial cell invasion and early-stage intestinal fitness in a dysbiotic mouse model mediated by type 1 fimbriae expression.
      Title: The CpxRA two-component system of adherent and invasive Escherichia coli contributes to epithelial cell invasion and early-stage intestinal fitness in a dysbiotic mouse model mediated by type 1 fimbriae expression.
    2. The sensor of the bacterial histidine kinase CpxA is a novel dimer of extracytoplasmic Per-ARNT-Sim domains.
      Title: The sensor of the bacterial histidine kinase CpxA is a novel dimer of extracytoplasmic Per-ARNT-Sim domains.
    3. Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.
      Title: Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.
    4. Loss of cell wall integrity genes cpxA and mrcB causes flocculation in Escherichia coli.
      Title: Loss of cell wall integrity genes cpxA and mrcB causes flocculation in Escherichia coli.
    5. Structural asymmetry does not indicate hemiphosphorylation in the bacterial histidine kinase CpxA.
      Title: Structural asymmetry does not indicate hemiphosphorylation in the bacterial histidine kinase CpxA.
    6. Overall, these data demonstrate that NlpE acts as a sentinel for two important envelope biogenesis processes, namely, lipoprotein sorting and oxidative folding, and they further establish NlpE as a bona fide member of the CpxA-CpxR two-component system.
      Title: The Lipoprotein NlpE Is a Cpx Sensor That Serves as a Sentinel for Protein Sorting and Folding Defects in the Escherichia coli Envelope.
    7. Study found that the CpxRA system regulates the virulence properties of Avian pathogenic Escherichia coli. Adherence, invasiveness, motility, production of type 1 fimbriae and biofilm were negatively affected in the DeltacpxA mutant indicating that the CpxA is required for full manifestation of these phenotypes.
      Title: The CpxRA stress response system regulates virulence features of avian pathogenic Escherichia coli.
    8. The CpxAR system is widely considered to be responsible for sensing attachment, specifically to hydrophobic surfaces. Using both single-cell and population-level analyses, we confirm RcsCDB activation upon surface contact, but find that the CpxAR system is not activated, in contrast to what had earlier been reported. Thus, the role of CpxAR in surface sensing and initiation of biofilm formation should be reconsidered.
      Title: Reassessing the role of the Escherichia coli CpxAR system in sensing surface contact.
    9. The cpxA mutant was attenuated for virulence in the kidney but not in the bladder or urine.
      Title: Evaluation of CpxRA as a Therapeutic Target for Uropathogenic Escherichia coli Infections.
    10. X-ray structures of the CpxA reveals allosteric switching mechanism, according to which CpxR binding to one CpxA protomer triggers autophosphorylation in the second protomer.
      Title: Structural Coupling between Autokinase and Phosphotransferase Reactions in a Bacterial Histidine Kinase.
    Submit: New GeneRIF Correction See all GeneRIFs (29)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphoprotein phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphorelay sensor kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables phosphorelay sensor kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphorelay sensor kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphorelay sensor kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cell adhesion IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell adhesion involved in biofilm formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to cell envelope stress TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in phosphorelay signal transduction system IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to radiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in signal transduction IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in signal transduction TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    sensor histidine kinase CpxA
    NP_418347.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4103602..4104975 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418347.1 sensor histidine kinase CpxA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418347.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A3R0MR61, A0A793CL63
      Conserved Domains (1) summary
      PRK09470
      Location:1 → 457
      cpxA; envelope stress sensor histidine kinase CpxA
    Nucleotide Protein
    Heading Accession and Version

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