U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    msbA ATP-binding lipopolysaccharide transport protein [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945530, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    msbA
    Official Full Name
    ATP-binding lipopolysaccharide transport protein
    Primary source
    ECOCYC:EG10613
    Locus tag
    b0914
    See related
    ASAP:ABE-0003113
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0905
    Summary
    MsbA PDB structures 1JSQ, 1PF4 and 1Z2R, and any conclusions derived from these structures, have been retracted due to a software data inversion error (Chang, 2006). [More information is available at EcoGene: EG10613]. Lipid A serves as a hydrophobic membrane anchor for the lipopolysaccharide, which is a major component of the outer leaflet of the outer membranes of Gram-negative bacteria. [More information is available at EcoCyc: EG10613].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (966621..968369)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene integration host factor subunit beta Neighboring gene metallo-beta-lactamase superfamily inner membrane protein YcaI Neighboring gene tetraacyldisaccharide 4'-kinase Neighboring gene interstrand DNA crosslink repair glycosylase Neighboring gene PF03966 family protein YcaR Neighboring gene 3-deoxy-manno-octulosonate cytidylyltransferase

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Lipopolysaccharide stabilizes the crystal packing of the ABC transporter MsbA. Reyes CL, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2005 Jul 1. PMID 16511120, Free PMC Article
    2. Function of Escherichia coli MsbA, an essential ABC family transporter, in lipid A and phospholipid biosynthesis. Zhou Z, et al. J Biol Chem, 1998 May 15. PMID 9575204
    3. Density gradient enrichment of Escherichia coli conditional msbA mutants. Doerrler WT. Appl Environ Microbiol, 2007 Dec. PMID 17965211, Free PMC Article
    4. Conformational motion of the ABC transporter MsbA induced by ATP hydrolysis. Borbat PP, et al. PLoS Biol, 2007 Oct. PMID 17927448, Free PMC Article
    5. Macromolecular diffusion of biological polymers measured by confocal fluorescence recovery after photobleaching. Gribbon P, et al. Biophys J, 1998 Aug. PMID 9675204, Free PMC Article

    See all (77) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells.
      Title: The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells.
    2. The conformational states of the ABC transporter MsbA in a lipid environment investigated by small-angle scattering using stealth carrier nanodiscs have been reported.
      Title: Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs.
    3. The ABC exporter MsbA can couple ATP hydrolysis to an adenylate kinase activity, where ADP is converted into AMP and ATP. Single-point mutations reveal that both ATPase and adenylate kinase mechanisms are associated with the same conserved motifs of the nucleotide-binding domain.
      Title: Coupled ATPase-adenylate kinase activity in ABC transporters.
    4. Data show that the conformational space of lipid ABC transporter MsbA changes upon vanadate trapping.
      Title: The effect of drug binding on specific sites in transmembrane helices 4 and 6 of the ABC exporter MsbA studied by DNP-enhanced solid-state NMR.
    5. Data show that the twisting motion of ATP-binding cassette transporter MsbA plays an important role for the rearrangement of transmembrane helices and the opening of transmembrane domains on the periplasmic side.
      Title: Allosteric transitions of ATP-binding cassette transporter MsbA studied by the adaptive anisotropic network model.
    6. Data indicate that E208A mutation interrupts the inward- to outward-facing conformational switch in ATP-binding cassette transport protein MsbA.
      Title: Molecular disruption of the power stroke in the ATP-binding cassette transport protein MsbA.
    7. Data show that the isolated MsbA nucleotide-binding domain hydrolyzed ATP with V(max) = 45 nmol mg(-1) min(-1), similar to the full-length transporter.
      Title: Time-resolved Fourier transform infrared spectroscopy of the nucleotide-binding domain from the ATP-binding Cassette transporter MsbA: ATP hydrolysis is the rate-limiting step in the catalytic cycle.
    8. Catalytic activity of MsbA reconstituted in nanodisc particles is modulated by remote interactions with the bilayer
      Title: Catalytic activity of MsbA reconstituted in nanodisc particles is modulated by remote interactions with the bilayer.
    9. analysis of the E506Q and H537A dysfunctional mutants in the E. coli ABC transporter MsbA
      Title: Characterization of the E506Q and H537A dysfunctional mutants in the E. coli ABC transporter MsbA.
    10. investigation of catalytic mechanism using mutants: L511P prevents effective ATP hydrolysis; D512G hydrolyzes ATP at rate 3-fold faster than wild type; both mutants bind ATP at near-wild-type levels but are unable to maintain cell viability
      Title: Effects of the L511P and D512G mutations on the Escherichia coli ABC transporter MsbA.
    Submit: New GeneRIF Correction See all GeneRIFs (20)

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ABC-type transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ABC-type xenobiotic transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP binding ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATPase-coupled lipid transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ATPase-coupled lipid transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATPase-coupled lipid transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables lipid binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipopolysaccharide floppase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within lipid translocation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in lipid transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipid transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within lipopolysaccharide transport EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within lipopolysaccharide transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in lipopolysaccharide transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in xenobiotic transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of ATP-binding cassette (ABC) transporter complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of ATP-binding cassette (ABC) transporter complex RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    part_of MsbA transporter complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in membrane HDA PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    ATP-binding lipopolysaccharide transport protein
    NP_415434.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      966621..968369
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415434.1 ATP-binding lipopolysaccharide transport protein [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415434.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376RMT3
      Conserved Domains (1) summary
      PRK11176
      Location:1 → 582
      PRK11176; lipid A ABC transporter ATP-binding protein/permease MsbA
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.