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    fabA beta-hydroxyacyl-acyl carrier protein dehydratase/isomerase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945568, updated on 3-Dec-2024

    Summary

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    Official Symbol
    fabA
    Official Full Name
    beta-hydroxyacyl-acyl carrier protein dehydratase/isomerase
    Primary source
    ECOCYC:EG10273
    Locus tag
    b0954
    See related
    ASAP:ABE-0003229
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0945
    Summary
    Binds TrxA (Kumar, 2004). [More information is available at EcoGene: EG10273].
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    Genomic context

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    Sequence:
    NC_000913.3 (1015952..1016470, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene intermembrane transport protein PqiB Neighboring gene intermembrane transport lipoprotein PqiC Neighboring gene ribosome modulation factor Neighboring gene putative ATP-dependent protease YcbZ Neighboring gene macrodomain Ter protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Control of Unsaturation in De Novo Fatty Acid Biosynthesis by FabA. Bartholow TG, et al. Biochemistry, 2022 Apr 5. PMID 35255690, Free PMC Article
    2. Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters. Feng Y, et al. Mol Microbiol, 2011 Apr. PMID 21276098, Free PMC Article
    3. Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation. Luo LH, et al. Biotechnol Lett, 2009 Dec. PMID 19685209
    4. Crystallization and preliminary X-ray analysis of beta-hydroxydecanoyl thiol ester dehydrase from Escherichia coli. Sharma A, et al. J Biol Chem, 1990 Mar 25. PMID 2180957
    5. Escherichia coli beta-hydroxydecanoyl thioester dehydrase reacts with native C10 acyl-acyl-carrier proteins of plant and bacterial origin. Guerra DJ, et al. Arch Biochem Biophys, 1990 Aug 1. PMID 2195995

    See all (44) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Control of Unsaturation in De Novo Fatty Acid Biosynthesis by FabA.
      Title: Control of Unsaturation in De Novo Fatty Acid Biosynthesis by FabA.
    2. The FabA is a soluble protein that has been relatively straightforward to express and to purify from cultured E. coli, FabZ has shown to be mostly insoluble and only partially active.
      Title: Fused dimerization increases expression, solubility, and activity of bacterial dehydratase enzymes.
    3. The studies highlight key interactions of transient ACP-FabA protein complexes and define the dynamic movements of ACP that facilitate substrate delivery and trigger complex dissociation.
      Title: Crystal structures of acyl carrier protein in complex with two catalytic partners show a dynamic role in cellular metabolism.
    4. Data indicate that despite the remarkably similar arrangements of promoter elements, FadR predominately regulates fabA expression whereas FabR is the dominant regulator of fabB expression.
      Title: Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters.
    5. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain.
      Title: Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic manipulation.
    6. the key reaction in UFA synthesis catalyzed by FabB is elongation of the cis-3-decenoyl-ACP produced by FabA.
      Title: Escherichia coli unsaturated fatty acid synthesis: complex transcription of the fabA gene and in vivo identification of the essential reaction catalyzed by FabB.
    7. N-terminus verified by Edman degradation on complete protein
      Title: Derived amino acid sequence and identification of active site residues of Escherichia coli beta-hydroxydecanoyl thioester dehydrase.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables trans-2-decenoyl-acyl-carrier-protein isomerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables trans-2-decenoyl-acyl-carrier-protein isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in fatty acid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fatty acid biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in unsaturated fatty acid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    beta-hydroxyacyl-acyl carrier protein dehydratase/isomerase
    NP_415474.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1015952..1016470 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415474.1 beta-hydroxyacyl-acyl carrier protein dehydratase/isomerase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415474.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X1NPF0, A0AAJ3P0N9
      Conserved Domains (1) summary
      PRK05174
      Location:1 → 172
      PRK05174; bifunctional 3-hydroxydecanoyl-ACP dehydratase/trans-2-decenoyl-ACP isomerase
    Nucleotide Protein
    Heading Accession and Version

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