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    mgsA methylglyoxal synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945574, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mgsA
    Official Full Name
    methylglyoxal synthase
    Primary source
    ECOCYC:G6497
    Locus tag
    b0963
    See related
    ASAP:ABE-0003259
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0954; mgs; yccG
    Summary
    Methylglyoxal synthase (MGS) catalyzes the apparently irreversible formation of methylglyoxal from glycerone phosphate. [More information is available at EcoCyc: G6497].
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    Genomic context

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    Sequence:
    NC_000913.3 (1026557..1027015, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF03733 family inner membrane protein YccF Neighboring gene DNA helicase IV Neighboring gene DUF2057 domain-containing protein YccT Neighboring gene putative HspQ acetyl donor

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mechanistic implications of methylglyoxal synthase complexed with phosphoglycolohydroxamic acid as observed by X-ray crystallography and NMR spectroscopy. Marks GT, et al. Biochemistry, 2001 Jun 12. PMID 11389594
    2. Methylglyoxal synthase. Cooper RA. Methods Enzymol, 1975. PMID 236480
    3. Proton transfer reactions of methylglyoxal synthase. Summers MC, et al. J Am Chem Soc, 1977 Jun 22. PMID 325056
    4. The purification and properties of Escherichia coli methylglyoxal synthase. Hopper DJ, et al. Biochem J, 1972 Jun. PMID 4563643, Free PMC Article
    5. Identification of catalytic bases in the active site of Escherichia coli methylglyoxal synthase: cloning, expression, and functional characterization of conserved aspartic acid residues. Saadat D, et al. Biochemistry, 1998 Jul 14. PMID 9665712

    See all (30) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Deletion of the mgsA gene (strain LY168) resulted in the co-metabolism of glucose and xylose, and accelerated the metabolism of a 5-sugar mixture (mannose, glucose, arabinose, xylose and galactose) to ethanol.
      Title: Deletion of methylglyoxal synthase gene (mgsA) increased sugar co-metabolism in ethanol-producing Escherichia coli.
    2. Deletion of the E. coli mgsA gene (together with deletions of other ferementation disruption genes) increased production of succinate
      Title: Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C.
    3. N-terminus verified by Edman degradation on complete protein
      Title: Identification of catalytic bases in the active site of Escherichia coli methylglyoxal synthase: cloning, expression, and functional characterization of conserved aspartic acid residues.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methylglyoxal synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables methylglyoxal synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methylglyoxal synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in methylglyoxal biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in methylglyoxal biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within methylglyoxal biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein hexamerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    methylglyoxal synthase
    NP_415483.2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1026557..1027015 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415483.2 methylglyoxal synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415483.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7L7E8H2
      Conserved Domains (1) summary
      PRK05234
      Location:8 → 148
      mgsA; methylglyoxal synthase; Validated
    Nucleotide Protein
    Heading Accession and Version

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