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    amiA N-acetylmuramoyl-L-alanine amidase A [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946916, updated on 3-Dec-2024

    Summary

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    Official Symbol
    amiA
    Official Full Name
    N-acetylmuramoyl-L-alanine amidase A
    Primary source
    ECOCYC:EG11823
    Locus tag
    b2435
    See related
    ASAP:ABE-0008032
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2430; yfeE
    Summary
    Verified Tat substrate: [More information is available at EcoGene: EG11823]. Escherichia coli contains three N-acetylmuramyl-L-alanine amidases named AmiA, B, and C involved in cell division by splitting the murein septum. [More information is available at EcoCyc: EG11823].
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    Genomic context

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    Sequence:
    NC_000913.3 (2552352..2553221)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1131 domain-containing lipoprotein YfeY Neighboring gene putative inner membrane protein Neighboring gene putative acetyltransferase YpeA Neighboring gene coproporphyrinogen III oxidase Neighboring gene putative DNA-binding transcriptional regulator EutR

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Studying a cell division amidase using defined peptidoglycan substrates. Lupoli TJ, et al. J Am Chem Soc, 2009 Dec 30. PMID 19957935, Free PMC Article
    2. Rapid beta-lactam-induced lysis requires successful assembly of the cell division machinery. Chung HS, et al. Proc Natl Acad Sci U S A, 2009 Dec 22. PMID 19995973, Free PMC Article
    3. Envelope-bound N-acetylmuramyl-L-alanine amidase of Escherichia coli K 12. Purification and properties of the enzyme. van Heijenoort J, et al. Eur J Biochem, 1975 Oct 15. PMID 1102308
    4. Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance. Tanaka K, et al. Mol Gen Genet, 1991 May. PMID 1903834
    5. Modulation of Escherichia coli N-acetylmuramoyl-L-alanine amidase activity by phosphatidylglycerol. Vanderwinkel E, et al. Biochim Biophys Acta, 1985 Jan 28. PMID 2857097

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. AmiA is a zinc metalloprotease that requires at least a tetrasaccharide glycopeptide substrate for cleavage.
      Title: Studying a cell division amidase using defined peptidoglycan substrates.
    2. Periplasmic amidases AmiC and AmiA, secreted by the twin arginine transport (Tat) pathway, are localized to the cytoplasm in Escherichia coli YqjA and YghB double mutant cells.
      Title: Inefficient Tat-dependent export of periplasmic amidases in an Escherichia coli strain with mutations in two DedA family genes.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables N-acetylmuramoyl-L-alanine amidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables N-acetylmuramoyl-L-alanine amidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables N-acetylmuramoyl-L-alanine amidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in FtsZ-dependent cytokinesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within cell division EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in cell wall organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cellular response to antibiotic EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in peptidoglycan catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in outer membrane-bounded periplasmic space IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in outer membrane-bounded periplasmic space ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    located_in periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    N-acetylmuramoyl-L-alanine amidase A
    NP_416930.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2552352..2553221
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416930.1 N-acetylmuramoyl-L-alanine amidase A [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416930.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T5Y666
      Conserved Domains (1) summary
      PRK10319
      Location:1 → 287
      PRK10319; N-acetylmuramoyl-L-alanine amidase AmiA
    Nucleotide Protein
    Heading Accession and Version

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