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    rnc RNase III [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947033, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rnc
    Official Full Name
    RNase III
    Primary source
    ECOCYC:EG10857
    Locus tag
    b2567
    See related
    ASAP:ABE-0008448
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2565
    Summary
    rnc is a non-essential gene (Takiff, 1989; Yu, 2000) and the failure to obtain a viable insertion (Baba, 2006) may be due to a polar effect on the downstream essential era gene. [More information is available at EcoGene: EG10857]. RNase III is an endonuclease that cleaves double-stranded RNA to yield 5'-phosphates and 3'-hydroxyls . [More information is available at EcoCyc: EG10857].
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    Genomic context

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    Sequence:
    NC_000913.3 (2703383..2704063, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene holo-[acyl-carrier-protein] synthase Neighboring gene pyridoxine 5'-phosphate synthase Neighboring gene recombination mediator protein RecO Neighboring gene 30S ribosomal subunit maturation GTPase Era Neighboring gene signal peptidase I Neighboring gene 30S ribosomal subunit biogenesis factor LepA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Analysis of mRNA decay and rRNA processing in Escherichia coli multiple mutants carrying a deletion in RNase III. Babitzke P, et al. J Bacteriol, 1993 Jan. PMID 8416898, Free PMC Article
    2. RNase III cleavage of Escherichia coli beta-galactosidase and tryptophan operon mRNA. Shen V, et al. J Bacteriol, 1982 Jun. PMID 6176575, Free PMC Article
    3. The cII-independent expression of the phage lambda int gene in RNase III-defective E. coli. Belfort M. Gene, 1980 Oct. PMID 6254850
    4. Functional inactivation of lac alpha-peptide mRNA by a factor that purifies that Escherichia coli RNase III. Shen V, et al. J Biol Chem, 1981 Feb 25. PMID 6257691
    5. Regulation of plasmid DNA synthesis: isolation and characterization of copy number mutants of miniR6-5 and miniF plasmids. Ely S, et al. Mol Gen Genet, 1981. PMID 6261084

    See all (152) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Uncovering a delicate balance between endonuclease RNase III and ribosomal protein S15 in E. coli ribosome assembly.
      Title: Uncovering a delicate balance between endonuclease RNase III and ribosomal protein S15 in E. coli ribosome assembly.
    2. Based on these findings, we generated a E38A/E65A/Q165A triple mutant of EcRNase III (EcEEQ) that functions as a bacterial Dicer and produces random, heterogeneous 22-nt-siRNA cocktails that are optimal for gene silencing studies
      Title: The molecular mechanism of dsRNA processing by a bacterial Dicer.
    3. In vivo RNA cleavage rules and target repertoire of RNase III in Escherichia coli have been reported.
      Title: In vivo cleavage rules and target repertoire of RNase III in Escherichia coli.
    4. Molecular dynamics simulations provide a mechanism for facilitated product release, in which the Ser33 phosphomonoester forms a salt bridge with the Arg95 guanidinium group, thereby weakening RNase III engagement of product.
      Title: Mechanism of Ribonuclease III Catalytic Regulation by Serine Phosphorylation.
    5. PNPase regulates its own expression via a reversible RNase III-independent pathway acting upstream from the RNase III-dependent branch.
      Title: RNase III-Independent Autogenous Regulation of Escherichia coli Polynucleotide Phosphorylase via Translational Repression.
    6. Authors propose that another major role for the Nus-modified transcription complex in rrn operons is as an RNA chaperone insuring co-ordination of 16S rRNA folding and RNase III processing that results in production of proper 30S ribosome subunits.
      Title: Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis in Escherichia coli.
    7. The proU mRNA is processed by RNase III and this processing is osmoregulated.
      Title: RNase III initiates rapid degradation of proU mRNA upon hypo-osmotic stress in Escherichia coli.
    8. findings reveal an RNase III-mediated regulatory pathway that functions to modulate corA expression and, in turn, the influx of metal ions transported by CorA in E. coli.
      Title: RNase III controls the degradation of corA mRNA in Escherichia coli.
    9. Data indicates that RNase III has a more widespread role in cellular RNA processing than previously envisioned.
      Title: Analysis of Escherichia coli RNase E and RNase III activity in vivo using tiling microarrays.
    10. Multiple enzymes are involved in the GadY-directed cleavage of the full-length mRNA including the double-strand RNA-specific endoribonuclease RNase III.
      Title: RNase III participates in GadY-dependent cleavage of the gadX-gadW mRNA.
    Submit: New GeneRIF Correction See all GeneRIFs (17)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables enzyme binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables rRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ribonuclease III activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ribonuclease III activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribonuclease III activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in RNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of gene expression IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    RNase III
    NP_417062.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2703383..2704063 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417062.1 RNase III [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417062.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      D2K8Z1
      Conserved Domains (1) summary
      PRK00102
      Location:6 → 210
      rnc; ribonuclease III; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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