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    fadH 2,4-dienoyl-CoA reductase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947594, updated on 3-Dec-2024

    Summary

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    Official Symbol
    fadH
    Official Full Name
    2,4-dienoyl-CoA reductase
    Primary source
    ECOCYC:G36
    Locus tag
    b3081
    See related
    ASAP:ABE-0010124
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3071; ygjL
    Summary
    FadH contains FMN, FAD, and a 4Fe-4S cluster. [More information is available at EcoGene: EG12723].
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    Genomic context

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    Sequence:
    NC_000913.3 (3231665..3233683)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YgjJ Neighboring gene glycoside hydrolase Neighboring gene antitoxin/DNA-binding transcriptional repressor HigA Neighboring gene ribosome-dependent mRNA interferase toxin HigB Neighboring gene 23S rRNA m(2)G1835 methyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Degradation of unsaturated fatty acids. Identification of intermediates in the degradation of cis-4-decenoly-CoA by extracts of beef-liver mitochondria. Kunau WH, et al. Eur J Biochem, 1978 Nov 15. PMID 729581
    2. 2,4-Dienoyl coenzyme A reductases from bovine liver and Escherichia coli. Comparison of properties. Dommes V, et al. J Biol Chem, 1984 Feb 10. PMID 6363415
    3. Cloning and expression of the fadH gene and characterization of the gene product 2,4-dienoyl coenzyme A reductase from Escherichia coli. He XY, et al. Eur J Biochem, 1997 Sep 1. PMID 9346310
    4. Evidence for the essential function of 2,4-dienoyl-coenzyme A reductase in the beta-oxidation of unsaturated fatty acids in vivo. Isolation and characterization of an Escherichia coli mutant with a defective 2,4-dienoyl-coenzyme A reductase. You SY, et al. J Biol Chem, 1989 Oct 5. PMID 2506179
    5. Overlapping repressor binding sites result in additive regulation of Escherichia coli FadH by FadR and ArcA. Feng Y, et al. J Bacteriol, 2010 Sep. PMID 20622065, Free PMC Article

    See all (17) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. We report mapping of the fadH promoter and document its complex regulation by three independent regulators, the fatty acid degradation FadR repressor, the oxygen-responsive ArcA-ArcB two-component system, and the cyclic AMP receptor protein-cyclic AMP.
      Title: Overlapping repressor binding sites result in additive regulation of Escherichia coli FadH by FadR and ArcA.
    2. supports a mechanism that has Tyr166 with the assistance of His252 acting as proton donor in the wild-type enzyme to produce 2-enoyl-CoA
      Title: Two distinct proton donors at the active site of Escherichia coli 2,4-dienoyl-CoA reductase are responsible for the formation of different products.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Cloning and expression of the fadH gene and characterization of the gene product 2,4-dienoyl coenzyme A reductase from Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 2,4-dienoyl-CoA reductase (NADPH) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 2,4-dienoyl-CoA reductase (NADPH) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 2,4-dienoyl-CoA reductase (NADPH) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 2,4-dienoyl-CoA reductase (NADPH) activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables FAD binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables FMN binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables FMN binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fatty acid beta-oxidation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fatty acid beta-oxidation, unsaturated, even number, reductase/isomerase pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within fatty acid beta-oxidation, unsaturated, even number, reductase/isomerase pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    2,4-dienoyl-CoA reductase
    NP_417552.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3231665..3233683
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417552.1 2,4-dienoyl-CoA reductase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417552.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T6PU12
      Conserved Domains (2) summary
      COG0446
      Location:355 → 665
      FadH2; NADPH-dependent 2,4-dienoyl-CoA reductase, sulfur reductase, or a related oxidoreductase [Lipid transport and metabolism]
      cd02930
      Location:6 → 358
      DCR_FMN; 2,4-dienoyl-CoA reductase (DCR) FMN-binding domain. DCR in E. coli is an iron-sulfur flavoenzyme which contains FMN, FAD, and a 4Fe-4S cluster. It is also a monomer, unlike that of its eukaryotic counterparts which form homotetramers and lack the ...
    Nucleotide Protein
    Heading Accession and Version

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