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    melB melibiose:H(+)/Na(+)/Li(+) symporter [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948635, updated on 3-Dec-2024

    Summary

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    Official Symbol
    melB
    Official Full Name
    melibiose:H(+)/Na(+)/Li(+) symporter
    Primary source
    ECOCYC:EG10578
    Locus tag
    b4120
    See related
    ASAP:ABE-0013495
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4113; mel-4
    Summary
    Major Facilitator Superfamily (MFS). [More information is available at EcoGene: EG10578]. MelB is a melibiose-cation cotransport protein belonging to the Glycoside-Pentoside-Hexuronide:Cation Symporter Family (GPH) . [More information is available at EcoCyc: EG10578].
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    Genomic context

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    Sequence:
    NC_000913.3 (4343369..4344790)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional dual regulator MelR Neighboring gene alpha-galactosidase Neighboring gene DUF2238 domain-containing inner membrane protein YjdF Neighboring gene fumarase B

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Consequences of hydrophobic mismatch between lipids and melibiose permease on melibiose transport. Dumas F, et al. Biochemistry, 2000 Apr 25. PMID 10769142
    2. An investigation of cysteine mutants on the cytoplasmic loop X/XI in the melibiose transporter of Escherichia coli by using thiol reagents: implication of structural conservation of charged residues. Ding PZ. Biochem Biophys Res Commun, 2003 Aug 8. PMID 12878191
    3. Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli. Yazyu H, et al. J Biol Chem, 1984 Apr 10. PMID 6323466
    4. Projection structure at 8 A resolution of the melibiose permease, an Na-sugar co-transporter from Escherichia coli. Hacksell I, et al. EMBO J, 2002 Jul 15. PMID 12110569, Free PMC Article
    5. Study of amide-proton exchange of Escherichia coli melibiose permease by attenuated total reflection-Fourier transform infrared spectroscopy: evidence of structure modulation by substrate binding. Dave N, et al. J Biol Chem, 2002 Feb 1. PMID 11729178

    See all (60) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Lys-377 and Ile-22 of melB are crucial for the substrates binding.
      Title: The Melibiose Transporter of Escherichia coli: CRITICAL CONTRIBUTION OF LYS-377 TO THE STRUCTURAL ORGANIZATION OF THE INTERACTING SUBSTRATE BINDING SITES.
    2. Data show that the regulatory protein IIA(Glc) directly binds to melibiose permease (MelB) with dissociation constant value 25 muM.
      Title: Insights into the inhibitory mechanisms of the regulatory protein IIA(Glc) on melibiose permease activity.
    3. MelB-N-ethylmaleimide difference spectra are proportionally less disrupted than the R141C ones
      Title: Alteration of sugar-induced conformational changes of the melibiose permease by mutating Arg141 in loop 4-5.
    4. Data describe the three-dimensional structure of melibiose permease at 10 angstrom resolution in the membrane plane with cryo-electron microscopy from two-dimensional crystals.
      Title: Three-dimensional structure of the sugar symporter melibiose permease from cryo-electron microscopy.
    5. Data suggest that loop 4-5 of melibiose permease contributes to coordinated interactions between ion and sugar binding sites, and participates in an electrogenic conformational transition essential for coupled substrate translocation.
      Title: The inner interhelix loop 4-5 of the melibiose permease from Escherichia coli takes part in conformational changes after sugar binding.
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    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables glycoside-pentoside-hexuronide:cation symporter activity RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    enables melibiose:monoatomic cation symporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables melibiose:sodium symporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methylgalactoside transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables symporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in carbohydrate transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in carbohydrate transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within melibiose transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within methylgalactoside transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in sodium ion transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in sodium ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    melibiose:H(+)/Na(+)/Li(+) symporter

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4343369..4344790
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418544.2 melibiose:H(+)/Na(+)/Li(+) symporter [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418544.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7I3I8
      Conserved Domains (1) summary
      PRK10429
      Location:1 → 473
      PRK10429; melibiose:sodium transporter MelB
    Nucleotide Protein
    Heading Accession and Version

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