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    gapA glyceraldehyde-3-phosphate dehydrogenase A [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947679, updated on 3-Dec-2024

    Summary

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    Official Symbol
    gapA
    Official Full Name
    glyceraldehyde-3-phosphate dehydrogenase A
    Primary source
    ECOCYC:EG10367
    Locus tag
    b1779
    See related
    ASAP:ABE-0005920
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1777; gad; gap1
    Summary
    A gapA mutant is not in the Keio collection; gapA mutants were not expected to grow on the medium used to attempt to isolate them; gapA is not an essential gene (Baba, 2006). [More information is available at EcoGene: EG10367]. Escherichia coli is unusual in having two glyceraldehyde-3-phosphate dehydrogenases. [More information is available at EcoCyc: EG10367].
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    Genomic context

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    Sequence:
    NC_000913.3 (1862771..1863766)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1315 domain-containing protein YeaC Neighboring gene methionine sulfoxide reductase B Neighboring gene putative aldose 1-epimerase YeaD Neighboring gene methylglyoxal reductase YeaE

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The strong efficiency of the Escherichia coli gapA P1 promoter depends on a complex combination of functional determinants. Thouvenot B, et al. Biochem J, 2004 Oct 15. PMID 15250823, Free PMC Article
    2. Mutant analysis of glyceraldehyde 3-phosphate dehydrogenase in Escherichia coli. Hillman JD. Biochem J, 1979 Apr 1. PMID 89843, Free PMC Article
    3. Nucleotide polymorphism and evolution in the glyceraldehyde-3-phosphate dehydrogenase gene (gapA) in natural populations of Salmonella and Escherichia coli. Nelson K, et al. Proc Natl Acad Sci U S A, 1991 Aug 1. PMID 1862091, Free PMC Article
    4. Molecular and evolutionary relationships among enteric bacteria. Lawrence JG, et al. J Gen Microbiol, 1991 Aug. PMID 1955870
    5. Role of the histidine 176 residue in glyceraldehyde-3-phosphate dehydrogenase as probed by site-directed mutagenesis. Soukri A, et al. Biochemistry, 1989 Mar 21. PMID 2659073

    See all (57) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Characterization and structure of glyceraldehyde-3-phosphate dehydrogenase type 1 from Escherichia coli.
      Title: Characterization and structure of glyceraldehyde-3-phosphate dehydrogenase type 1 from Escherichia coli.
    2. The authors report that GAPDH of these pathogens is ADP-ribosylated either in the cytoplasm or in the extracellular medium. GAPDH catalyzes its own modification, which involves Cys-149 at the active site.
      Title: NAD+-dependent post-translational modification of Escherichia coli glyceraldehyde-3-phosphate dehydrogenase.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Direct detection of potential selenium delivery proteins by using an Escherichia coli strain unable to incorporate selenium from selenite into proteins.
    4. Data indicate that exported glyceraldehyde-3-phosphate dehydrogenase may act as a virulence factor which could contribute to bacterial pathogenesis.
      Title: Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli: interaction of the extracellular enzyme with human plasminogen and fibrinogen.
    5. promoter gapA P1 is a very efficient promoter sharing common properties with both extended -10 and non-extended -10 promoters
      Title: The strong efficiency of the Escherichia coli gapA P1 promoter depends on a complex combination of functional determinants.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables NAD binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NAD binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NAD binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables NADP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in glucose metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in glycolytic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within glycolytic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 

    General protein information

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    Preferred Names
    glyceraldehyde-3-phosphate dehydrogenase A
    NP_416293.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1862771..1863766
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416293.1 glyceraldehyde-3-phosphate dehydrogenase A [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416293.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A793CDN0
      Conserved Domains (1) summary
      PRK15425
      Location:1 → 331
      gapA; glyceraldehyde-3-phosphate dehydrogenase
    Nucleotide Protein
    Heading Accession and Version

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