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    alaE L-alanine exporter [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947147, updated on 3-Dec-2024

    Summary

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    Official Symbol
    alaE
    Official Full Name
    L-alanine exporter
    Primary source
    ECOCYC:G7399
    Locus tag
    b2670
    See related
    ASAP:ABE-0008793
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2664; ygaW
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    Genomic context

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    Sequence:
    NC_000913.3 (2799164..2799613)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene thiosulfate sulfurtransferase YgaP Neighboring gene DNA-binding transcriptional repressor StpA with RNA chaperone activity Neighboring gene DUF2002 domain-containing protein YgaC Neighboring gene DUF883 domain-containing protein YgaM

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Importance of transmembrane helix 4 of l-alanine exporter AlaE in oligomer formation and substrate export activity in Escherichia coli. Ihara K, et al. Microbiology (Reading), 2022 Mar. PMID 35275050
    2. L-Alanine Exporter, AlaE, of Escherichia coli Functions as a Safety Valve to Enhance Survival under Feast Conditions. Katsube S, et al. Int J Mol Sci, 2019 Oct 7. PMID 31591285, Free PMC Article
    3. Impact of charged amino acid substitution in the transmembrane domain of L-alanine exporter, AlaE, of Escherichia coli on the L-alanine export. Kim S, et al. Arch Microbiol, 2017 Jan. PMID 27572251
    4. Characterization of the l-alanine exporter AlaE of Escherichia coli and its potential role in protecting cells from a toxic-level accumulation of l-alanine and its derivatives. Kim S, et al. Microbiologyopen, 2015 Aug. PMID 26073055, Free PMC Article
    5. Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. Ihara K, et al. J Biosci Bioeng, 2017 Apr. PMID 28057466

    See all (13) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Importance of transmembrane helix 4 of l-alanine exporter AlaE in oligomer formation and substrate export activity in Escherichia coli.
      Title: Importance of transmembrane helix 4 of l-alanine exporter AlaE in oligomer formation and substrate export activity in Escherichia coli.
    2. Study provides evidence that the L-alanine exporter AlaE plays a critical role in avoiding a toxic accumulation of L-alanine and functions as the 'safety valve' under potential high L-alanine environments, such as in animal intestines.
      Title: L-Alanine Exporter, AlaE, of Escherichia coli Functions as a Safety Valve to Enhance Survival under Feast Conditions.
    3. the gene expression of the alaE is regulated by Lrp in response to intracellular levels of l-alanine, which eventually leads to intracellular homeostasis of l-alanine concentrations
      Title: Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli.
    4. Charged amino acid residues (R45 and D84) in the AlaE transmembrane domain play a pivotal role in L-alanine export.
      Title: Impact of charged amino acid substitution in the transmembrane domain of L-alanine exporter, AlaE, of Escherichia coli on the L-alanine export.
    5. These data suggested that the AlaE-mediated l-alanine extrusion was driven by proton motive force.
      Title: Characterization of the l-alanine exporter AlaE of Escherichia coli and its potential role in protecting cells from a toxic-level accumulation of l-alanine and its derivatives.
    6. On the basis of these results, the authors concluded that YgaW is likely to be the physiologically most relevant exporter for L-alanine in Escherichia coli and proposed that the gene be redesignated alaE for alanine export.
      Title: Inducible L-alanine exporter encoded by the novel gene ygaW (alaE) in Escherichia coli.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables L-amino acid efflux transmembrane transporter activity EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables L-amino acid efflux transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables L-amino acid efflux transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in L-alanine transport EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in L-alanine transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in L-alpha-amino acid transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in amino acid export across plasma membrane EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in amino acid export across plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in amino acid transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cellular response to stress IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    L-alanine exporter

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2799164..2799613
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417156.1 L-alanine exporter [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417156.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7A6ZJY0, A0A7D7EUZ4
      Conserved Domains (1) summary
      pfam06610
      Location:3 → 141
      AlaE; L-alanine exporter
    Nucleotide Protein
    Heading Accession and Version

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