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    hycI hydrogenase 3 maturation protease [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947428, updated on 3-Dec-2024

    Summary

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    Official Symbol
    hycI
    Official Full Name
    hydrogenase 3 maturation protease
    Primary source
    ECOCYC:G7414
    Locus tag
    b2717
    See related
    ASAP:ABE-0008931
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2712
    Summary
    FhlA regulon. [More information is available at EcoGene: EG13396]. HycI is a protease that cleaves the carboxy-terminus of HycE, modifying it into its final form as the large subunit of hydrogenase 3 . [More information is available at EcoCyc: G7414].
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    Genomic context

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    Sequence:
    NC_000913.3 (2842573..2843043, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene beta-glucoside specific PTS enzyme IIBC component Neighboring gene 6-phospho-beta-glucosidase AscB Neighboring gene formate hydrogenlyase assembly protein Neighboring gene formate hydrogenlyase subunit HycG Neighboring gene formate hydrogenlyase subunit HycF Neighboring gene formate hydrogenlyase subunit HycE

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Activation of a [NiFe]-hydrogenase-4 isoenzyme by maturation proteases. Finney AJ, et al. Microbiology (Reading), 2020 Sep. PMID 32731905, Free PMC Article
    2. Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli. Kumarevel T, et al. Biochem Biophys Res Commun, 2009 Nov 13. PMID 19720045
    3. Solution structure and backbone dynamics of an endopeptidase HycI from Escherichia coli: implications for mechanism of the [NiFe] hydrogenase maturation. Yang F, et al. J Biol Chem, 2007 Feb 9. PMID 17150961
    4. Fidelity of metal insertion into hydrogenases. Magalon A, et al. FEBS Lett, 2001 Jun 15. PMID 11418115
    5. Analysis of the cleavage site specificity of the endopeptidase involved in the maturation of the large subunit of hydrogenase 3 from Escherichia coli. Theodoratou E, et al. Arch Microbiol, 2000 Feb. PMID 10795682

    See all (16) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Activation of a [NiFe]-hydrogenase-4 isoenzyme by maturation proteases.
      Title: Activation of a [NiFe]-hydrogenase-4 isoenzyme by maturation proteases.
    2. the crystal structure of HycI at 1.70 A resolution was presented.
      Title: Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Characterisation of a protease from Escherichia coli involved in hydrogenase maturation.
    4. First solution structure of M52 family peptidase HycI is reported; the backbone dynamics is well correlated with the structural and functional basis of substrate recognition.
      Title: Solution structure and backbone dynamics of an endopeptidase HycI from Escherichia coli: implications for mechanism of the [NiFe] hydrogenase maturation.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables aspartic-type endopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables endopeptidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables endopeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables enzyme activator activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in protein processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein processing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    hydrogenase 3 maturation protease

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2842573..2843043 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417197.1 hydrogenase 3 maturation protease [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417197.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T5YBF7
      Conserved Domains (1) summary
      PRK11544
      Location:1 → 156
      hycI; hydrogenase 3 maturation protease; Provisional
    Nucleotide Protein
    Heading Accession and Version

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