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    waaC ADP-heptose:LPS heptosyltransferase 1 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948136, updated on 3-Dec-2024

    Summary

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    Official Symbol
    waaC
    Official Full Name
    ADP-heptose:LPS heptosyltransferase 1
    Primary source
    ECOCYC:EG11189
    Locus tag
    b3621
    See related
    ASAP:ABE-0011847
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3611; rfa-2; rfaC; yibC
    Summary
    Overexpression causes abnormal biofilm architecture. [More information is available at EcoGene: EG11189]. The lipopolysaccharide of Escherichia coli K-12 consists of two major components: the hydrophobic lipid A moiety inserted into the outer membrane and the phosphorylated core oligosaccharide . [More information is available at EcoCyc: EG11189].
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    Genomic context

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    Sequence:
    NC_000913.3 (3795979..3796938)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ADP-L-glycero-D-mannoheptose 6-epimerase Neighboring gene ADP-heptose--LPS heptosyltransferase 2 Neighboring gene O-antigen ligase Neighboring gene protein YibX;protein YibX-S Neighboring gene protein YibY

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Escherichia coli heptosyltransferase I: investigation of protein dynamics of a GT-B structural enzyme. Czyzyk DJ, et al. Biochemistry, 2013 Aug 6. PMID 23865375, Free PMC Article
    2. Lipopolysaccharide biosynthesis without the lipids: recognition promiscuity of Escherichia coli heptosyltransferase I. Czyzyk DJ, et al. Biochemistry, 2011 Dec 13. PMID 22059588, Free PMC Article
    3. Kdo-(2 --> 8)-Kdo-(2 --> 4)-Kdo but not Kdo-(2 --> 4)-Kdo-(2 --> 4)-Kdo is an acceptor for transfer of L-glycero-alpha-D-manno-heptose by Escherichia coli heptosyltransferase I (WaaC). Gronow S, et al. Innate Immun, 2009 Feb. PMID 19201821
    4. Discovery of new Gram-negative antivirulence drugs: structure and properties of novel E. coli WaaC inhibitors. Moreau F, et al. Bioorg Med Chem Lett, 2008 Jul 15. PMID 18571407
    5. Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and ADP-2-deoxy-2-fluoro heptose. Grizot S, et al. J Mol Biol, 2006 Oct 20. PMID 16963083

    See all (33) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. HepI enzyme undergoing a substrate binding-induced transition from an open to closed state prior to catalysis.
      Title: Escherichia coli heptosyltransferase I: investigation of protein dynamics of a GT-B structural enzyme.
    2. Among the nine mutants resistant to coliphage infection that authors selected, six were in the fhuA gene, two were mutated in the waaC gene, and one was mutated in the gmhD gene.
      Title: A novel strategy to isolate cell-envelope mutants resistant to phage infection: bacteriophage mEp213 requires lipopolysaccharides in addition to FhuA to enter Escherichia coli K-12.
    3. catalytic efficiency of Heptosyltransferase I with the fully deacylated analogue of Escherichia coli HepI LipidA
      Title: Lipopolysaccharide biosynthesis without the lipids: recognition promiscuity of Escherichia coli heptosyltransferase I.
    4. WaaC had access to the substrate before WaaA transfer was completed.
      Title: Kdo-(2 --> 8)-Kdo-(2 --> 4)-Kdo but not Kdo-(2 --> 4)-Kdo-(2 --> 4)-Kdo is an acceptor for transfer of L-glycero-alpha-D-manno-heptose by Escherichia coli heptosyltransferase I (WaaC).
    5. N-terminus verified by Edman degradation on complete protein
      Title: Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis.
    6. Results report the structure of WaaC alone at 1.9 A resolution, and in complex with either ADP or the non-cleavable analog ADP-2-deoxy-2-fluoro-heptose of the sugar donor at 2.4 A resolution.
      Title: Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and ADP-2-deoxy-2-fluoro heptose.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ADP-heptose-lipopolysaccharide heptosyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lipopolysaccharide heptosyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipopolysaccharide heptosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in lipopolysaccharide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipopolysaccharide core region biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in lipopolysaccharide core region biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within lipopolysaccharide core region biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in lipopolysaccharide core region biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    ADP-heptose:LPS heptosyltransferase 1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3795979..3796938
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418078.1 ADP-heptose:LPS heptosyltransferase 1 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418078.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A485J7M5
      Conserved Domains (1) summary
      PRK10964
      Location:1 → 319
      PRK10964; lipopolysaccharide heptosyltransferase RfaC
    Nucleotide Protein
    Heading Accession and Version

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