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    mnmG 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948248, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mnmG
    Official Full Name
    5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG
    Primary source
    ECOCYC:EG10375
    Locus tag
    b3741
    See related
    ASAP:ABE-0012231
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3734; gidA; trmF
    Summary
    mnmG function is based on genetic experiments showing tht it is in the same pathway as mnmE and that mnmE is epistatic to mnmG. [More information is available at EcoGene: EG10375]. MnmG and MnmE both act in a tRNA modification pathway that reduces +2 frameshift errors in translation . [More information is available at EcoCyc: EG10375].
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    Genomic context

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    Sequence:
    NC_000913.3 (3923744..3925633, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ATP synthase accessory factor AtpI Neighboring gene 16S rRNA m(7)G527 methyltransferase Neighboring gene flavoprotein MioC Neighboring gene DNA-binding transcriptional dual regulator AsnC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structure-function analysis of Escherichia coli MnmG (GidA), a highly conserved tRNA-modifying enzyme. Shi R, et al. J Bacteriol, 2009 Dec. PMID 19801413, Free PMC Article
    2. Nucleotide sequence of Escherichia coli K-12 replication origin. Sugimoto K, et al. Proc Natl Acad Sci U S A, 1979 Feb. PMID 370832, Free PMC Article
    3. Concurrent transcription from the gid and mioC promoters activates replication of an Escherichia coli minichromosome. Ogawa T, et al. Mol Gen Genet, 1991 Nov. PMID 1745229
    4. The AT richness and gid transcription determine the left border of the replication origin of the E. coli chromosome. Asai T, et al. EMBO J, 1990 Dec. PMID 2249664, Free PMC Article
    5. Stabilization of G domain conformations in the tRNA-modifying MnmE-GidA complex observed with double electron electron resonance spectroscopy. Böhme S, et al. J Biol Chem, 2010 May 28. PMID 20353943, Free PMC Article

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. deletion of the mnmE or mnmG gene results in complete inhibition of growth unless the medium contains polyamines
      Title: Absolute requirement for polyamines for growth of Escherichia coli mutants (mnmE/G) defective in modification of the wobble anticodon of transfer-RNA.
    2. Investigation of the activities and the tRNA specificity of the MnmG enzyme.
      Title: The output of the tRNA modification pathways controlled by the Escherichia coli MnmEG and MnmC enzymes depends on the growth conditions and the tRNA species.
    3. GidA binding induces large conformational and dynamic changes in MnmE
      Title: Stabilization of G domain conformations in the tRNA-modifying MnmE-GidA complex observed with double electron electron resonance spectroscopy.
    4. Limited trypsinolysis of MnmG suggests significant conformational changes upon FAD binding.
      Title: Structure-function analysis of Escherichia coli MnmG (GidA), a highly conserved tRNA-modifying enzyme.
    5. MnmE and GidA form a functional complex in the tRNA modification process in which both proteins are interdependent.
      Title: Further insights into the tRNA modification process controlled by proteins MnmE and GidA of Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables flavin adenine dinucleotide binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables flavin adenine dinucleotide binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables flavin adenine dinucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in regulation of cytoplasmic translational fidelity NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within response to UV IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA methylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within tRNA methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA wobble uridine modification IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within tRNA wobble uridine modification IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA wobble uridine modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA wobble uridine modification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic tRNA wobble base thiouridylase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3923744..3925633 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418197.1 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418197.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A377C9H7, A0A8S7FB94
      Conserved Domains (1) summary
      PRK05192
      Location:3 → 628
      PRK05192; tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG
    Nucleotide Protein
    Heading Accession and Version

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