lexA DNA-binding transcriptional repressor LexA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

Go to the top of the page Help

Official Symbol: lexA
Official Full Name: DNA-binding transcriptional repressor LexA
Primary source: ECOCYC:EG10533
Locus tag: b4043
See related: ASAP:ABE-0013241
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK4035; exrA; spr; tsl; umuA
Summary: LexA autocleavage, stimulated by RecA, of the first 84 aa of LexA removes the DNA binding region and is required to activate the SOS response. [More information is available at EcoGene: EG10533]. LexA is a protein that belongs to the LexA family . [More information is available at EcoCyc: EG10533].
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

Sequence:: NC_000913.3 (4257115..4257723)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Non-canonical LexA proteins regulate the SOS response in the Bacteroidetes.
Title: Non-canonical LexA proteins regulate the SOS response in the Bacteroidetes.
Single-molecule imaging of LexA degradation in Escherichia coli elucidates regulatory mechanisms and heterogeneity of the SOS response.
Title: Single-molecule imaging of LexA degradation in Escherichia coli elucidates regulatory mechanisms and heterogeneity of the SOS response.
The Parameter-Fitness Landscape of lexA Autoregulation in Escherichia coli.
Title: The Parameter-Fitness Landscape of lexA Autoregulation in Escherichia coli.
Two components of DNA replication-dependent LexA cleavage.
Title: Two components of DNA replication-dependent LexA cleavage.
YbiB from Escherichia coli, the defining member of the novel Trpd2 family of prokaryotic DNA-binding proteins, forms an operon with the DNA helicase gene dinG and is under LexA control.
Title: YbiB from Escherichia coli, the Defining Member of the Novel TrpD2 Family of Prokaryotic DNA-binding Proteins.
Like the self-cleaving serine proteases LexA and UmuD in Escherichia coli, UmuDAb required RecA for cleavage.
Title: The Acinetobacter regulatory UmuDAb protein cleaves in response to DNA damage with chimeric LexA/UmuD characteristics.
These results suggest that, IscR stabilizes LexA at the cka promoter after DNA damage thus, preventing its cleavage and inactivation, and this cooperation ensures that suicidal colicin K production is switched on only as a last resort.
Title: Double locking of an Escherichia coli promoter by two repressors prevents premature colicin expression and cell lysis.
These results reveal specific amino acid determinants of the RecA-LexA interaction and suggest that LexA binds RecA and RecAi+6 at a composite site on the RecA filament, which could explain the role of the active filament during LexA cleavage.
Title: Separation of recombination and SOS response in Escherichia coli RecA suggests LexA interaction sites.
The conformational flexibility of unbound LexA is the key element in establishing a co-ordinated SOS response.
Title: Interconversion between bound and free conformations of LexA orchestrates the bacterial SOS response.
LexA regulated genes exhibit phenotypic heterogeneity as high level expression is observed in only a small subpopulation.
Title: Genes regulated by the Escherichia coli SOS repressor LexA exhibit heterogeneous expression.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription repressor activity	IPI Inferred from Physical Interaction more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables serine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables transcription cis-regulatory region binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in SOS response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within SOS response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	EXP Inferred from Experiment more info	PubMed
involved_in negative regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of DNA-templated transcription	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of protein-DNA complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: DNA-binding transcriptional repressor LexA

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

4257115..4257723

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418467.1 DNA-binding transcriptional repressor LexA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418467.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A8S0FHY8

Conserved Domains (1) summary

PRK00215
Location:3 → 199

PRK00215; transcriptional repressor LexA