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    ppa inorganic pyrophosphatase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948748, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ppa
    Official Full Name
    inorganic pyrophosphatase
    Primary source
    ECOCYC:EG10755
    Locus tag
    b4226
    See related
    ASAP:ABE-0013824
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4222
    Summary
    The enzyme is a hexamer, arranged as a dimer of trimers in the crystal structure . [More information is available at EcoCyc: EG10755].
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    Genomic context

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    Sequence:
    NC_000913.3 (4449122..4449652, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene gamma-glutamylamine cyclotransferase family protein YtfP Neighboring gene ChpS antitoxin of the ChpB-ChpS toxin-antitoxin system Neighboring gene endoribonuclease toxin ChpB Neighboring gene galactofuranose ABC transporter periplasmic binding protein Neighboring gene galactofuranose ABC transporter putative ATP binding subunit

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. X-ray crystallographic studies of recombinant inorganic pyrophosphatase from Escherichia coli. Oganessyan VYu, et al. FEBS Lett, 1994 Jul 18. PMID 8034059
    2. Engineering a new magnesium binding site in the subunit contact region of Escherichia coli inorganic pyrophosphatase. Parfenyev AN, et al. Biochemistry (Mosc), 2000 Mar. PMID 10739482
    3. Mechanism of Ca2+-induced inhibition of Escherichia coli inorganic pyrophosphatase. Avaeva SM, et al. Biochemistry (Mosc), 2000 Mar. PMID 10739481
    4. Active site interactions in oligomeric structures of inorganic pyrophosphatases. Avaeva SM. Biochemistry (Mosc), 2000 Mar. PMID 10739480
    5. Reciprocal effects of substitutions at the subunit interfaces in hexameric pyrophosphatase of Escherichia coli. Dimeric and monomeric forms of the enzyme. Salminen A, et al. J Biol Chem, 1999 Nov 26. PMID 10567351

    See all (65) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data indicate that the pyrophosphatase activities of non-halophilic Escherichia coli was 0.1 M NaCl.
      Title: Constant enthalpy change value during pyrophosphate hydrolysis within the physiological limits of NaCl.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: '98 Escherichia coli SWISS-2DPAGE database update.
    3. We further determined the crystal structure of the Arg43Gln mutant variant of E-PPase complexed with one phosphate and four Mn ions.
      Title: Reversible inhibition of Escherichia coli inorganic pyrophosphatase by fluoride: trapped catalytic intermediates in cryo-crystallographic studies.
    4. Results demonstrate that the flexibility of the loops where the residues Gly100 and Gly147 are located is necessary at the stages of substrate binding and product release.
      Title: Substitutions of glycine residues Gly100 and Gly147 in conservative loops decrease rates of conformational rearrangements of Escherichia coli inorganic pyrophosphatase.
    5. Results show that the substitutions of Gly100 and Gly147 result in overall destabilization of the globular structure.
      Title: Effect of mutation of the conservative glycine residues Gly100 and Gly147 on stability of Escherichia coli inorganic pyrophosphatase.
    6. Results report a novel human protein, calphoglin, which activates inorganic pyrophosphatase (IPP) and enhances phosphoglucomutase activity through the activated IPP.
      Title: Cellular signaling mediated by calphoglin-induced activation of IPP and PGM.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables inorganic diphosphate phosphatase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables inorganic diphosphate phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables inorganic diphosphate phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables inorganic triphosphate phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in phosphate-containing compound metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in phosphate-containing compound metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 

    General protein information

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    Preferred Names
    inorganic pyrophosphatase
    NP_418647.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4449122..4449652 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418647.1 inorganic pyrophosphatase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418647.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A775TFT7
      Conserved Domains (1) summary
      PRK01250
      Location:1 → 176
      PRK01250; inorganic diphosphatase
    Nucleotide Protein
    Heading Accession and Version

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