lipA lipoyl synthase [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

Official Symbol: lipA
Official Full Name: lipoyl synthase
Primary source: ECOCYC:EG11306
Locus tag: b0628
See related: ASAP:ABE-0002155
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0621; lip
Summary: Radical SAM superfamily. [More information is available at EcoGene: EG11306]. LipA is required for the step of lipoate biosynthesis at which sulfur is inserted into the octanoyl side-chain of an octanoylated E2 domain, to form the lipoate moiety . [More information is available at EcoCyc: EG11306].
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NC_000913.3 Genomic Context describing neighboring genes

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The physiological and molecular characterization of a small colony variant of Escherichia coli and its phenotypic rescue with lipoic acid identifies LipA mutation as a cause for stress resistance and abnormal phenotype.
Title: The Physiological and Molecular Characterization of a Small Colony Variant of Escherichia coli and Its Phenotypic Rescue.
These data show that PdhR plays no physiological role in regulation of lipA expression.
Title: PdhR, the pyruvate dehydrogenase repressor, does not regulate lipoic acid synthesis.
N-terminus verified by Edman degradation on mature peptide
Title: Escherichia coli LipA is a lipoyl synthase: in vitro biosynthesis of lipoylated pyruvate dehydrogenase complex from octanoyl-acyl carrier protein.

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IDA Inferred from Direct Assay more info	PubMed
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables lipoate synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables lipoate synthase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in lipoate biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipoate biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

NP_415161.1

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