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    mdfA multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945448, updated on 17-Dec-2024

    Summary

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    Official Symbol
    mdfA
    Official Full Name
    multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
    Primary source
    ECOCYC:G6440
    Locus tag
    b0842
    See related
    ASAP:ABE-0002871
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    cmlA; cmr; ECK0832
    Summary
    Major Facilitator Superfamily (MFS). [More information is available at EcoGene: EG13696]. The MdfA protein, also known as Cmr, is a multidrug efflux protein belonging to the major facilitator superfamily (MFS) . [More information is available at EcoCyc: G6440].
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    Genomic context

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    Sequence:
    NC_000913.3 (883673..884905)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional repressor DeoR Neighboring gene undecaprenyl pyrophosphate phosphatase Neighboring gene uncharacterized protein YbjH Neighboring gene 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase Neighboring gene inner membrane protein YbjJ

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Membrane topology of the multidrug transporter MdfA: complementary gene fusion studies reveal a nonessential C-terminal domain. Adler J, et al. J Bacteriol, 2002 Jun. PMID 12029048, Free PMC Article
    2. 3D model of the Escherichia coli multidrug transporter MdfA reveals an essential membrane-embedded positive charge. Sigal N, et al. Biochemistry, 2005 Nov 15. PMID 16274234
    3. Do physiological roles foster persistence of drug/multidrug-efflux transporters? A case study. Krulwich TA, et al. Nat Rev Microbiol, 2005 Jul. PMID 15953929
    4. The chloramphenicol resistance gene cmlA is disseminated on transferable plasmids that confer multiple-drug resistance in swine Escherichia coli. Bischoff KM, et al. FEMS Microbiol Lett, 2005 Feb 1. PMID 15668031
    5. Promiscuity in the geometry of electrostatic interactions between the Escherichia coli multidrug resistance transporter MdfA and cationic substrates. Adler J, et al. J Biol Chem, 2005 Jan 28. PMID 15557318

    See all (54) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Enhanced internal ionic interaction of MFS efflux pump MdfA contributes to its elevated antibiotic export.
      Title: Enhanced internal ionic interaction of MFS efflux pump MdfA contributes to its elevated antibiotic export.
    2. Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels.
      Title: Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels.
    3. analysis of MdfA variant I239T/G354E in complexes with three electrically different ligands reveals the molecular basis for substrate recognition
      Title: Structure of an engineered multidrug transporter MdfA reveals the molecular basis for substrate recognition.
    4. Outward open conformation of the Major Facilitator Superfamily multidrug/H(+) antiporter MdfA from Escherichia coli has changes in relative orientations of the N- and C-terminal lobes, and transmembrane helix5 (TM5) is kinked and twisted. In vitro reconstitution experiments demonstrate the importance of selected residues for transport and molecular dynamics simulations provide insights into antiporter switching.
      Title: Outward open conformation of a Major Facilitator Superfamily multidrug/H(+) antiporter provides insights into switching mechanism.
    5. MdfA has exceptional promiscuous capabilities not only in its interaction with a plethora of chemically dissimilar compounds, but also concerning the various faces of its modes of energy consumption. [review]
      Title: The fascinating but mysterious mechanistic aspects of multidrug transport by MdfA from Escherichia coli.
    6. Crystallization and X-ray diffraction analysis of the multidrug-resistance transporter MdfA from Escherichia coli has been reported.
      Title: The multidrug-resistance transporter MdfA from Escherichia coli: crystallization and X-ray diffraction analysis.
    7. The MdfA structure consists of four pseudo-symmetrical, three-helix repeats, consistent with the typical structure of an MFS transporter.
      Title: Substrate-bound structure of the E. coli multidrug resistance transporter MdfA.
    8. The mdfA gene can function as transporter responsible for multidrug resistance, and also mediated the synergistic function with its surrounding genes in conjugative plasmids.
      Title: Structural and environmental features of novel mdfA variant and mdfA genes in recombinant regions of Escherichia coli.
    9. GlpG is able to recognize and cleave truncated forms of MdfA but not the intact protein.
      Title: Cleavage of a multispanning membrane protein by an intramembrane serine protease.
    10. the Mdr-binding pocket of MdfA is conformationally sensitive
      Title: A promiscuous conformational switch in the secondary multidrug transporter MdfA.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables potassium:proton antiporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sodium:proton antiporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables sodium:proton antiporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in potassium ion transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proton transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of cellular pH IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to antibiotic IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to antibiotic IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in sodium ion transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in xenobiotic detoxification by transmembrane export across the plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in xenobiotic detoxification by transmembrane export across the plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in xenobiotic detoxification by transmembrane export across the plasma membrane IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      883673..884905
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415363.1 multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415363.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X1NHZ1, A0AAN3TR86
      Conserved Domains (1) summary
      PRK15402
      Location:7 → 410
      PRK15402; MdfA family multidrug efflux MFS transporter
    Nucleotide Protein
    Heading Accession and Version

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