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    ompF outer membrane porin F [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945554, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ompF
    Official Full Name
    outer membrane porin F
    Primary source
    ECOCYC:EG10671
    Locus tag
    b0929
    See related
    ASAP:ABE-0003153
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    cmlB; cry; ECK0920; nfxB; tolF
    Summary
    Trimeric. [More information is available at EcoGene: EG10671]. OmpF is a member of the General Bacterial Porin (GBP) family. [More information is available at EcoCyc: EG10671].
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    Genomic context

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    Sequence:
    NC_000913.3 (985894..986982, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydroxyacylglutathione hydrolase GloC Neighboring gene aspartate aminotransferase Neighboring gene asparagine--tRNA ligase Neighboring gene nicotinate phosphoribosyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Subconductance states in OmpF gating. Baslé A, et al. Biochim Biophys Acta, 2004 Jul 1. PMID 15238263
    2. Detergent binding in trigonal crystals of OmpF porin from Escherichia coli. Penel S, et al. Biochimie, 1998 May-Jun. PMID 9782392
    3. Stability of trimeric OmpF porin: the contributions of the latching loop L2. Phale PS, et al. Biochemistry, 1998 Nov 10. PMID 9843370
    4. Organic solvent tolerance of Escherichia coli is independent of OmpF levels in the membrane. Asako H, et al. Appl Environ Microbiol, 1999 Jan. PMID 9872794, Free PMC Article
    5. OmpF changes and the complexity of Escherichia coli adaptation to prolonged lactose limitation. Zhang E, et al. FEMS Microbiol Lett, 1999 Jul 15. PMID 10427722

    See all (177) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Study of the early response of Escherichia coli lpcA and ompF mutants to ciprofloxacin.
      Title: Study of the early response of Escherichia coli lpcA and ompF mutants to ciprofloxacin.
    2. Designer Outer Membrane Protein Facilitates Uptake of Decoy Molecules into a Cytochrome P450BM3-Based Whole-Cell Biocatalyst.
      Title: Designer Outer Membrane Protein Facilitates Uptake of Decoy Molecules into a Cytochrome P450BM3-Based Whole-Cell Biocatalyst.
    3. A protocol for production of perdeuterated OmpF porin for neutron crystallography.
      Title: A protocol for production of perdeuterated OmpF porin for neutron crystallography.
    4. Porin threading drives receptor disengagement and establishes active colicin transport through Escherichia coli OmpF.
      Title: Porin threading drives receptor disengagement and establishes active colicin transport through Escherichia coli OmpF.
    5. OmpF Downregulation Mediated by Sigma E or OmpR Activation Confers Cefalexin Resistance in Escherichia coli in the Absence of Acquired beta-Lactamases.
      Title: OmpF Downregulation Mediated by Sigma E or OmpR Activation Confers Cefalexin Resistance in Escherichia coli in the Absence of Acquired β-Lactamases.
    6. Trimeric porins, such as ompF, have specific lipopolysaccharide binding sites that are essential for porin biogenesis.
      Title: Gram-negative trimeric porins have specific LPS binding sites that are essential for porin biogenesis.
    7. Klebsiella pneumoniae OmpK35 and OmpK36 produced larger more permeable channels than their Escherichia coli homologs OmpF and OmpC.
      Title: Klebsiella pneumoniae Major Porins OmpK35 and OmpK36 Allow More Efficient Diffusion of β-Lactams than Their Escherichia coli Homologs OmpF and OmpC.
    8. The results suggest that trimerization is crucial for both structure and function of general porin OmpF in E. coli, whereas being trimer in substrate-specific channel OprP supports a pore function in Pseudomonas aeruginosa.
      Title: Why do the outer membrane proteins OmpF from E. coli and OprP from P. aeruginosa prefer trimers? Simulation studies.
    9. Two different centered monoclinic crystals of the E. coli outer-membrane protein OmpF originate from the same building block
      Title: Two different centered monoclinic crystals of the E. coli outer-membrane protein OmpF originate from the same building block.
    10. The site of lipopolysaccharide binding means that ColN will preferably bind at the interface and thus position itself close to the surface of its translocon component, OmpF.
      Title: The antibacterial toxin colicin N binds to the inner core of lipopolysaccharide and close to its translocator protein.
    Submit: New GeneRIF Correction See all GeneRIFs (26)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables colicin transmembrane transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables disordered domain specific binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables lipid binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipopolysaccharide binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables monoatomic ion channel activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables porin activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables porin activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables porin activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in bacteriocin transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in monoatomic ion transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in monoatomic ion transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in monoatomic ion transmembrane transport TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in monoatomic ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein homotrimerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of monoatomic ion channel complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of pore complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of pore complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    outer membrane porin F

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      985894..986982 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415449.1 outer membrane porin F [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415449.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      C9E721
      Conserved Domains (1) summary
      PRK10002
      Location:1 → 362
      PRK10002; porin OmpF
    Nucleotide Protein
    Heading Accession and Version

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