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    sufS L-cysteine desulfurase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946185, updated on 3-Dec-2024

    Summary

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    Official Symbol
    sufS
    Official Full Name
    L-cysteine desulfurase
    Primary source
    ECOCYC:G6906
    Locus tag
    b1680
    See related
    ASAP:ABE-0005610
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    csdB; ECK1676; ynhB
    Summary
    Fur regulon. [More information is available at EcoGene: EG13962]. SufS exhibits activity with respect to assembly of the ferredoxin iron-sulfur cluster in an in vitro assay . [More information is available at EcoCyc: G6906].
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    Genomic context

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    Sequence:
    NC_000913.3 (1759303..1760523, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene L,D-transpeptidase LdtE Neighboring gene sulfur carrier protein SufE Neighboring gene Fe-S cluster scaffold complex subunit SufD Neighboring gene Fe-S cluster scaffold complex subunit SufC Neighboring gene Fe-S cluster scaffold complex subunit SufB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues. Blahut M, et al. J Biol Chem, 2019 Aug 16. PMID 31248989, Free PMC Article
    2. Doublet translocation at GGA is mediated directly by mutant tRNA(2Gly). Pagel FT, et al. J Bacteriol, 1992 Jun. PMID 1597432, Free PMC Article
    3. Suppression of a -1 frameshift mutation by a recessive tRNA suppressor which causes doublet decoding. O'Mahony DJ, et al. J Bacteriol, 1989 Jul. PMID 2472379, Free PMC Article
    4. Mutants of translational components that alter reading frame by two steps forward or one step back. Falahee MB, et al. J Biol Chem, 1988 Dec 5. PMID 2848024
    5. A nifS-like gene, csdB, encodes an Escherichia coli counterpart of mammalian selenocysteine lyase. Gene cloning, purification, characterization and preliminary x-ray crystallographic studies. Mihara H, et al. J Biol Chem, 1999 May 21. PMID 10329673

    See all (42) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The beta-latch structural element of the SufS cysteine desulfurase mediates active site accessibility and SufE transpersulfurase positioning.
      Title: The β-latch structural element of the SufS cysteine desulfurase mediates active site accessibility and SufE transpersulfurase positioning.
    2. a detailed model of the SufS-catalyzed reaction from Cys binding to C-S bond cleavage and indicate that Arg-56, His-123, and Cys-364 are critical SufS residues in this C-S bond cleavage pathway.
      Title: Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues.
    3. SufS possesses a conserved beta-hairpin structure plays a role in mediating interactions with SufE protein. This influences communication between active sites of the SufS dimer coordinating the shift between desulfurase and transpersulfurase activities.
      Title: Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS.
    4. The role of SufS is restricted to Fe-S cluster biosynthesis in Escherichia coli.
      Title: The Role of SufS Is Restricted to Fe-S Cluster Biosynthesis in Escherichia coli.
    5. These results show that dynamic changes may shift SufE to a sulfur-acceptor state that interacts more strongly with SufS.
      Title: SufE D74R Substitution Alters Active Site Loop Dynamics To Further Enhance SufE Interaction with the SufS Cysteine Desulfurase.
    6. Studies identified SufE-SufS and SufE-SufB interactions.
      Title: An in vivo method for characterization of protein interactions within sulfur trafficking systems of E. coli.
    7. N-terminus verified by Edman degradation on complete protein
      Title: A nifS-like gene, csdB, encodes an Escherichia coli counterpart of mammalian selenocysteine lyase. Gene cloning, purification, characterization and preliminary x-ray crystallographic studies.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cysteine desulfurase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cysteine desulfurase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cysteine sulfinate desulfinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pyridoxal phosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pyridoxal phosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables selenocysteine lyase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables selenocysteine lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables selenocysteine lyase activity NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in cysteine metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within iron-sulfur cluster assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within selenium compound metabolic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within sulfur compound metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    L-cysteine desulfurase
    NP_416195.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1759303..1760523 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416195.1 L-cysteine desulfurase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416195.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T6PK01
      Conserved Domains (1) summary
      PRK09295
      Location:1 → 406
      PRK09295; cysteine desulfurase SufS
    Nucleotide Protein
    Heading Accession and Version

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