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    sufA iron-sulfur cluster insertion protein SufA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 949014, updated on 3-Dec-2024

    Summary

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    Official Symbol
    sufA
    Official Full Name
    iron-sulfur cluster insertion protein SufA
    Primary source
    ECOCYC:EG11378
    Locus tag
    b1684
    See related
    ASAP:ABE-0005624
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1680; ydiC
    Summary
    iscA, sufA, erpA and nfuA are paralogs. [More information is available at EcoGene: EG11378]. SufA is part of the protein machinery that is involved in the biosynthesis of iron-sulfur clusters. [More information is available at EcoCyc: EG11378].
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    Genomic context

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    Sequence:
    NC_000913.3 (1764018..1764386, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene Fe-S cluster scaffold complex subunit SufD Neighboring gene Fe-S cluster scaffold complex subunit SufC Neighboring gene Fe-S cluster scaffold complex subunit SufB Neighboring gene ncRNA Neighboring gene uncharacterized protein YdiH

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Native Escherichia coli SufA, coexpressed with SufBCDSE, purifies as a [2Fe-2S] protein and acts as an Fe-S transporter to Fe-S target enzymes. Gupta V, et al. J Am Chem Soc, 2009 May 6. PMID 19366265, Free PMC Article
    2. Assignment of the (1)H, (15)N and (13)C resonances of SufA from Escherichia coli involved in Fe-S cluster biosynthesis. Duraffourg N, et al. J Biomol NMR, 2004 Nov. PMID 15754061
    3. Deletion of the Proposed Iron Chaperones IscA/SufA Results in Accumulation of a Red Intermediate Cysteine Desulfurase IscS in Escherichia coli. Yang J, et al. J Biol Chem, 2015 May 29. PMID 25907559, Free PMC Article
    4. A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coli. Outten FW, et al. Mol Microbiol, 2004 May. PMID 15101990
    5. Complementary roles of SufA and IscA in the biogenesis of iron-sulfur clusters in Escherichia coli. Lu J, et al. Biochem J, 2008 Jan 15. PMID 17941825

    See all (33) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Ferric uptake regulator (Fur) reversibly binds a [2Fe-2S] cluster to sense intracellular iron homeostasis in Escherichia coli.
      Title: Ferric uptake regulator (Fur) reversibly binds a [2Fe-2S] cluster to sense intracellular iron homeostasis in Escherichia coli.
    2. deletion of IscA/SufA may disrupt the iron delivery for iron-sulfur cluster biogenesis, therefore impeding sulfur delivery by IscS, and result in the accumulation of red IscS in E. coli cells
      Title: Deletion of the Proposed Iron Chaperones IscA/SufA Results in Accumulation of a Red Intermediate Cysteine Desulfurase IscS in Escherichia coli.
    3. Results suggest that the biogenesis of the [4Fe-4S] and the [2Fe-2S] clusters may have distinct pathways and that IscA/SufA paralogues are essential for the [4Fe-4S] cluster assembly, but are dispensable for the [2Fe-2S] cluster assembly.
      Title: IscA/SufA paralogues are required for the [4Fe-4S] cluster assembly in enzymes of multiple physiological pathways in Escherichia coli under aerobic growth conditions.
    4. Under aerobiosis, IscA and SufA are functionally redundant carriers and both can receive an Fe/S cluster from IscU.
      Title: Iron-sulfur (Fe/S) protein biogenesis: phylogenomic and genetic studies of A-type carriers.
    5. SufA may constitute the redundant cellular activities to recruit intracellular iron and deliver iron for the iron-sulfur cluster assembly in E. coli.
      Title: Complementary roles of SufA and IscA in the biogenesis of iron-sulfur clusters in Escherichia coli.
    6. SufA exists as a homodimer; four cysteine residues in the Cys-Gly-Cys motif are positioned in close proximity at the dimer interface; these cysteines together with the nearby Glu118 may allow coordination of an Fe-S cluster and/or an Fe atom.
      Title: Crystal structure of Escherichia coli SufA involved in biosynthesis of iron-sulfur clusters: implications for a functional dimer.
    7. SufA transcription was activated directly by binding of IscR and OxyR to the promoter.
      Title: IscR acts as an activator in response to oxidative stress for the suf operon encoding Fe-S assembly proteins.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 2 iron, 2 sulfur cluster binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 2 iron, 2 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 2 iron, 2 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron-sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in iron-sulfur cluster assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in iron-sulfur cluster assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within iron-sulfur cluster assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein maturation by iron-sulfur cluster transfer IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein maturation by iron-sulfur cluster transfer IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within response to oxidative stress IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to oxidative stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of iron-sulfur cluster transfer complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    iron-sulfur cluster insertion protein SufA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1764018..1764386 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416199.1 iron-sulfur cluster insertion protein SufA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416199.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AI91
      Conserved Domains (1) summary
      PRK09504
      Location:1 → 122
      sufA; iron-sulfur cluster assembly scaffold protein; Provisional
    Nucleotide Protein
    Heading Accession and Version

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