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    glpT sn-glycerol 3-phosphate:phosphate antiporter [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946704, updated on 17-Dec-2024

    Summary

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    Official Symbol
    glpT
    Official Full Name
    sn-glycerol 3-phosphate:phosphate antiporter
    Primary source
    ECOCYC:EG10401
    Locus tag
    b2240
    See related
    ASAP:ABE-0007401
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2232
    Summary
    GlpT exhanges cytoplasmic phosphate for glycerol phosphate. [More information is available at EcoGene: EG10401]. GlpT is the major Escherichia coli uptake system for glycerol-3-phosphate, and this transporter belongs to the Major Facilitator Superfamily (MFS) of transporters . [More information is available at EcoCyc: EG10401].
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    Genomic context

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    Sequence:
    NC_000913.3 (2351016..2352374, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene pseudo Neighboring gene glycerophosphoryl diester phosphodiesterase GlpQ Neighboring gene anaerobic glycerol-3-phosphate dehydrogenase subunit A Neighboring gene anaerobic glycerol-3-phosphate dehydrogenase subunit B Neighboring gene anaerobic glycerol-3-phosphate dehydrogenase subunit C

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The transmembrane topology of the sn-glycerol-3-phosphate permease of Escherichia coli analysed by phoA and lacZ protein fusions. Gött P, et al. Mol Microbiol, 1988 Sep. PMID 3141744
    2. Functional characterization of cysteine residues in GlpT, the glycerol 3-phosphate transporter of Escherichia coli. Fann MC, et al. J Bacteriol, 2003 Jul. PMID 12813080, Free PMC Article
    3. Structural biology. Breaching the barrier. Locher KP, et al. Science, 2003 Aug 1. PMID 12893929
    4. Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli. Huang Y, et al. Science, 2003 Aug 1. PMID 12893936
    5. High-yield expression and functional analysis of Escherichia coli glycerol-3-phosphate transporter. Auer M, et al. Biochemistry, 2001 Jun 5. PMID 11380257

    See all (73) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Elevated expression of GlpT and UhpT via FNR activation contributes to increased fosfomycin susceptibility in Escherichia coli under anaerobic conditions.
      Title: Elevated Expression of GlpT and UhpT via FNR Activation Contributes to Increased Fosfomycin Susceptibility in Escherichia coli under Anaerobic Conditions.
    2. Data indicate that mutating noninteracting residues to alanine have no effect on the pulling force of C-terminal transmembrane helices (TMHs) in membrane transport proteins CaiT, BtuC, NhaA, EmrD and GlpT.
      Title: Cotranslational folding of membrane proteins probed by arrest-peptide-mediated force measurements.
    3. studies suggest that only a few amino acid residues that line the GlpT transporter lumen act as specificity determinants
      Title: Structural basis of substrate selectivity in the glycerol-3-phosphate: phosphate antiporter GlpT.
    4. simulations highlight the cavity-lining helices as being significantly flexible, suggesting that the transport mechanism may involve intra-helix motions in addition to pseudo-rigid body motions of the N- and C-terminal domains relative to one another
      Title: Helix dynamics in a membrane transport protein: comparative simulations of the glycerol-3-phosphate transporter and its constituent helices.
    5. Here we report the 3.3 A resolution structure of GlpT, the glycerol-3-phosphate transporter from the E. coli inner membrane, in the absence of a substrate and propose that GlpT operates via a single binding-site, alternating-access mechanism.
      Title: Crystal structure and mechanism of GlpT, the glycerol-3-phosphate transporter from E. coli.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables glucose 6-phosphate:phosphate antiporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables glycerol-3-phosphate transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glycerol-phosphate:phosphate antiporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables organophosphate:phosphate antiporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in glucose-6-phosphate transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in glycerol metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within glycerol transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within glycerol-3-phosphate transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in glycerol-3-phosphate transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in phosphate ion transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    sn-glycerol 3-phosphate:phosphate antiporter

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2351016..2352374 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416743.1 sn-glycerol 3-phosphate:phosphate antiporter [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416743.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376M537, A0A8S7EAF3
      Conserved Domains (1) summary
      PRK11273
      Location:1 → 452
      glpT; glycerol-3-phosphate transporter
    Nucleotide Protein
    Heading Accession and Version

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