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    rbn ribonuclease BN [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946760, updated on 17-Dec-2024

    Summary

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    Official Symbol
    rbn
    Official Full Name
    ribonuclease BN
    Primary source
    ECOCYC:G7175
    Locus tag
    b2268
    See related
    ASAP:ABE-0007496
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2262; ecoZ; elaC; rnz; zipD
    Summary
    RNase BN is required for MazF-mediated cell death after inhibition of translation (Amitai, 2009). yihY was previously thought to be rbn. [More information is available at EcoGene: EG14260]. RNase BN, also identified as binuclear zinc phosphodiesterase, cleaves the 3'-terminal portion of tRNAs as well as various short unstructured RNAs. [More information is available at EcoCyc: G7175].
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    Genomic context

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    Sequence:
    NC_000913.3 (2381608..2382525)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene tail-anchored inner membrane protein ElaB Neighboring gene putative acetyltransferase ElaA Neighboring gene protease ElaD Neighboring gene IPR002035/DUF3520 domain-containing protein YfbK

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A mutant of escherichia coli defective in removing 3' terminal nucleotides from some transfer RNA precursor molecules. Seidman JG, et al. Cell, 1975 Aug. PMID 1098779
    2. Mode of action of RNase BN/RNase Z on tRNA precursors: RNase BN does not remove the CCA sequence from tRNA. Dutta T, et al. J Biol Chem, 2010 Jul 23. PMID 20489203, Free PMC Article
    3. Catalytic properties of RNase BN/RNase Z from Escherichia coli: RNase BN is both an exo- and endoribonuclease. Dutta T, et al. J Biol Chem, 2009 Jun 5. PMID 19366704, Free PMC Article
    4. Ribonuclease BN: identification and partial characterization of a new tRNA processing enzyme. Asha PK, et al. Proc Natl Acad Sci U S A, 1983 Jun. PMID 6344080, Free PMC Article
    5. Exosite modules guide substrate recognition in the ZiPD/ElaC protein family. Schilling O, et al. J Biol Chem, 2005 May 6. PMID 15699034

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. These data indicate that RNase BN plays an important role in determining levels of the global transcription regulator, 6S RNA, throughout the growth cycle.
      Title: Growth Phase-dependent Variation of RNase BN/Z Affects Small RNAs: REGULATION OF 6S RNA.
    2. metal selectivity of ZiPD only partially relies on first shell metal ligands
      Title: Zinc- and iron-dependent cytosolic metallo-beta-lactamase domain proteins exhibit similar zinc-binding affinities, independent of an atypical glutamate at the metal-binding site.
    3. A ZiPD exosite is identified that guides physiological substrate recognition in the ZiPD/ElaC protein family.
      Title: Exosite modules guide substrate recognition in the ZiPD/ElaC protein family.
    4. the first crystal structure of ZiPD determined by multiple anomalous diffraction at a resolution of 2.9 A
      Title: The crystal structure of the zinc phosphodiesterase from Escherichia coli provides insight into function and cooperativity of tRNase Z-family proteins.
    5. the RNase Z homologue encoded by Escherichia coli elaC gene is RNase BN
      Title: The RNase Z homologue encoded by Escherichia coli elaC gene is RNase BN.
    6. These results suggest a potentially important new role for the RNase Z family of proteins in RNA metabolism, particularly in organisms lacking RNase E.
      Title: RNase Z in Escherichia coli plays a significant role in mRNA decay.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3'-tRNA processing endoribonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3'-tRNA processing endoribonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA endonuclease activity, producing 5'-phosphomonoesters IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA endonuclease activity, producing 5'-phosphomonoesters IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA exonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA exonuclease activity, producing 5'-phosphomonoesters IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables exonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein homodimerization activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in tRNA 3'-end processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA processing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    ribonuclease BN
    NP_416771.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2381608..2382525
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416771.4 ribonuclease BN [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416771.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7EAN6
      Conserved Domains (1) summary
      TIGR02649
      Location:3 → 305
      true_RNase_BN; ribonuclease BN
    Nucleotide Protein
    Heading Accession and Version

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    Other Literature Sources
    Molecular Biology Databases
    Research Materials