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    iscS cysteine desulfurase IscS [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947004, updated on 17-Dec-2024

    Summary

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    Official Symbol
    iscS
    Official Full Name
    cysteine desulfurase IscS
    Primary source
    ECOCYC:G7325
    Locus tag
    b2530
    See related
    ASAP:ABE-0008324
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2527; nuvC; yfhO; yzzO
    Summary
    IscS desulfurase from cysteine via a Cys328 persulfide to binding partners as a transpersulfidase with ThiI Cys456 for tRNA s(4)U8 modification and with SirA(TusA) Cys19 to thiolate tRNA U34. [More information is available at EcoGene: EG12677]. Cysteine desulfurase (IcsC) catalyzes the transfer of sulfur and selenium from cysteine and selenocysteine to a range of recipients. [More information is available at EcoCyc: G7325].
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    Genomic context

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    Sequence:
    NC_000913.3 (2660317..2661531, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene iron-sulfur cluster insertion protein IscA Neighboring gene scaffold protein for iron-sulfur cluster assembly Neighboring gene DNA-binding transcriptional dual regulator IscR Neighboring gene tRNA Cm32/Um32 methyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Regulation of Escherichia coli IscS desulfurase activity by ferrous iron and cysteine. Wu G, et al. Biochem Biophys Res Commun, 2008 Sep 19. PMID 18639525
    2. The cysteine-desulfurase IscS promotes the production of the rhodanese RhdA in the persulfurated form. Forlani F, et al. FEBS Lett, 2005 Dec 19. PMID 16310786
    3. Bacterial cysteine desulfurases: their function and mechanisms. Mihara H, et al. Appl Microbiol Biotechnol, 2002 Oct. PMID 12382038
    4. Preliminary crystallographic analysis of the cysteine desulfurase IscS from Escherichia coli. Urbina HD, et al. Acta Crystallogr D Biol Crystallogr, 2002 Jul. PMID 12077450
    5. Repair of nitric oxide-modified ferredoxin [2Fe-2S] cluster by cysteine desulfurase (IscS). Yang W, et al. J Biol Chem, 2002 Apr 12. PMID 11825893

    See all (79) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. human NFS1 was almost fully able to complement the role of IscS in Moco biosynthesis when its specific interaction partner protein MOCS3 from humans was also present.
      Title: Functional Complementation Studies Reveal Different Interaction Partners of Escherichia coli IscS and Human NFS1.
    2. TusA (YhhP) and IscS are required for molybdenum cofactor-dependent base-analog detoxification.
      Title: TusA (YhhP) and IscS are required for molybdenum cofactor-dependent base-analog detoxification.
    3. study determined the crystal structures of IscS-IscU and IscS-TusA complexes providing the first insight into their different modes of binding and the mechanism of sulfur transfer
      Title: Structural basis for Fe-S cluster assembly and tRNA thiolation mediated by IscS protein-protein interactions.
    4. CyaY participates in iron-sulfur (Fe-S) cluster assembly as an iron-dependent inhibitor of cluster formation, through binding to the desulfurase IscS.
      Title: Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS.
    5. The desulfurase activity of IscS is gradually inhibited as the amount of iron and sulfide bound to IscS increases.
      Title: Regulation of Escherichia coli IscS desulfurase activity by ferrous iron and cysteine.
    6. These results provide a clue to the possible role of iscS in pyrimidine metabolism by gene regulation.
      Title: The iscS gene deficiency affects the expression of pyrimidine metabolism genes.
    7. N-terminus verified by Edman degradation on complete protein
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 2 iron, 2 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cysteine desulfurase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cysteine desulfurase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables pyridoxal phosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pyridoxal phosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables selenocysteine lyase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sulfur carrier activity EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in L-cysteine catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in L-cysteine catabolic process NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in [2Fe-2S] cluster assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in detection of UV NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in iron-sulfur cluster assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within iron-sulfur cluster assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in iron-sulfur cluster assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within oxazole or thiazole biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in selenocysteine catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in sulfur compound transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within tRNA 4-thiouridine biosynthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA wobble position uridine thiolation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in thiamine biosynthetic process NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of IscS-IscU complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of IscS-TusA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of L-cysteine desulfurase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of sulfurtransferase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    cysteine desulfurase IscS
    YP_026169.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2660317..2661531 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. YP_026169.1 cysteine desulfurase IscS [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for YP_026169.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A829LCS2
      Conserved Domains (1) summary
      PRK14012
      Location:1 → 404
      PRK14012; IscS subfamily cysteine desulfurase
    Nucleotide Protein
    Heading Accession and Version

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