U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    dcuB anaerobic C4-dicarboxylate transporter DcuB [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948641, updated on 17-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    dcuB
    Official Full Name
    anaerobic C4-dicarboxylate transporter DcuB
    Primary source
    ECOCYC:EG10006
    Locus tag
    b4123
    See related
    ASAP:ABE-0013503
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4116; genF
    Summary
    IT (Ion Transporter) superfamily (Prakash, 2003). [More information is available at EcoGene: EG10006]. The DcuB transporter is one of three transporters known to be responsible for the uptake of C4-dicarboxylates such as fumarate under anaerobic conditions. [More information is available at EcoCyc: EG10006].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (4347404..4348744, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF2238 domain-containing inner membrane protein YjdF Neighboring gene fumarase B Neighboring gene DNA-binding transcriptional activator DcuR Neighboring gene sensor histidine kinase DcuS Neighboring gene PF06902 family protein YjdI

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. A Mutational Hotspot and Strong Selection Contribute to the Order of Mutations Selected for during Escherichia coli Adaptation to the Gut. Lourenço M, et al. PLoS Genet, 2016 Nov. PMID 27812114, Free PMC Article
    2. Conversion of the sensor kinase DcuS of Escherichia coli of the DcuB/DcuS sensor complex to the C(4) -dicarboxylate responsive form by the transporter DcuB. Wörner S, et al. Environ Microbiol, 2016 Dec. PMID 27318186
    3. The fumarate/succinate antiporter DcuB of Escherichia coli is a bifunctional protein with sites for regulation of DcuS-dependent gene expression. Kleefeld A, et al. J Biol Chem, 2009 Jan 2. PMID 18957436
    4. Influence of C(4) -Dcu transporters on hydrogenase and formate dehydrogenase activities in stationary phase-grown fermenting Escherichia coli. Karapetyan L, et al. IUBMB Life, 2020 Aug. PMID 32277802
    5. Anaerobic fumarate transport in Escherichia coli by an fnr-dependent dicarboxylate uptake system which is different from the aerobic dicarboxylate uptake system. Engel P, et al. J Bacteriol, 1992 Sep. PMID 1512189, Free PMC Article

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Influence of C4 -Dcu transporters on hydrogenase and formate dehydrogenase activities in stationary phase-grown fermenting Escherichia coli.
      Title: Influence of C(4) -Dcu transporters on hydrogenase and formate dehydrogenase activities in stationary phase-grown fermenting Escherichia coli.
    2. The relationship of absolute contents of DcuS, DcuB, and DctA to the functional state of DcuS revealed their contribution to the control of DcuS-DcuR in vivo, which was not accessible by other approaches, leading to a revision of previous models.
      Title: Cellular Concentrations of the Transporters DctA and DcuB and the Sensor DcuS of Escherichia coli and the Contributions of Free and Complexed DcuS to Transcriptional Regulation by DcuR.
    3. in vivo competitive assays showed that one of the second steps (focA) exhibited positive epistasis with the first, while another (dcuB) exhibited negative epistasis. The data shows that strong effect adaptive mutations continuously recur in gut commensal bacterial species.
      Title: A Mutational Hotspot and Strong Selection Contribute to the Order of Mutations Selected for during Escherichia coli Adaptation to the Gut.
    4. Deleting both dcuB and dcuC genes resulted in 90% decrease of succinate titer, suggesting that DcuB and DcuC were the main transporters for succinate efflux.
      Title: Activating C4-dicarboxylate transporters DcuB and DcuC for improving succinate production.
    Submit: New GeneRIF Correction

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables C4-dicarboxylate transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables C4-dicarboxylate transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables antiporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables succinate:fumarate antiporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables symporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within C4-dicarboxylate transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in C4-dicarboxylate transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within anaerobic respiration EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in fumarate transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in succinate transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    anaerobic C4-dicarboxylate transporter DcuB

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4347404..4348744 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418547.1 anaerobic C4-dicarboxylate transporter DcuB [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418547.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7D7KDE4, A0A8S7JZC2
      Conserved Domains (1) summary
      PRK12489
      Location:38 → 444
      PRK12489; anaerobic C4-dicarboxylate transporter; Reviewed
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.