cadC DNA-binding transcriptional activator CadC [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: cadC
Official Full Name: DNA-binding transcriptional activator CadC
Primary source: ECOCYC:EG10133
Locus tag: b4133
See related: ASAP:ABE-0013532
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK4127
Summary: cadC transcription is repressed by multicopy leuO. [More information is available at EcoGene: EG10133]. CadC is a metal-sensitive transcriptional activator that regulates the expression of genes involved in cadaverine synthesis and excretion under low external pH and high concentrations of lysine . [More information is available at EcoCyc: EG10133].
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Genomic context

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Sequence:: NC_000913.3 (4360396..4361934, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Here, the authors present the crystal structure of the CadC DNA-binding domain (DBD) and show that it adopts a winged helix-turn-helix fold.
Title: Structure-function analysis of the DNA-binding domain of a transmembrane transcriptional activator.
The disordered CadC linker is required for transducing the pH-dependent response of the periplasmic sensor into a structural rearrangement that facilitates dimerization of the cytoplasmic CadC DNA-binding domain.
Title: Structural and Functional Analysis of the Signal-Transducing Linker in the pH-Responsive One-Component System CadC of Escherichia coli.
Upon induction, lysine-dependent conformational changes in LysP transduce the lysine signal via a direct conformational coupling to CadC without resolving the interaction completely.
Title: New insights into the interplay between the lysine transporter LysP and the pH sensor CadC in Escherichia coli.
Substitution of Cys208 and/or Cys272 by alanine resulted in CadC derivatives that were active in response to only one stimulus, either lysine or pH 5.8.
Title: Detection and function of an intramolecular disulfide bond in the pH-responsive CadC of Escherichia coli.
Results describe the crystal structure of CadC(pd) (residues 188-512) solved at a resolution of 1.8 A via multiple wavelength anomalous dispersion (MAD) using a ReCl(6)(2-) derivative.
Title: Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family.
The authors present evidence that CadC negatively regulates the expression of the arginine-dependent adaptive acid-resistance system (AR3), encoded by the adi locus while it has no effect on the expression of AR1 and AR2 systems.
Title: Interference of the CadC regulator in the arginine-dependent acid resistance system of Shigella and enteroinvasive E. coli.
The results suggest a model according to which CadC is inactivated by an interaction with LysP at a low external lysine concentration.
Title: The membrane-integrated transcriptional activator CadC of Escherichia coli senses lysine indirectly via the interaction with the lysine permease LysP.
The transcriptional activator CadC in Escherichia coli, a member of the ToxR-like proteins, activates transcription of the cadBA operon encoding the lysine decarboxylase CadA and the lysine-cadaverine antiporter CadB
Title: CadC-mediated activation of the cadBA promoter in Escherichia coli.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables phosphorelay response regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription cis-regulatory region binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in phosphorelay signal transduction system	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of protein-DNA complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: DNA-binding transcriptional activator CadC

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

4360396..4361934 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418557.1 DNA-binding transcriptional activator CadC [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418557.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A8T6B455

Conserved Domains (1) summary

PRK10153
Location:1 → 512

PRK10153; DNA-binding transcriptional activator CadC; Provisional