PHO4 phosphate-sensing transcription factor PHO4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PHO4
Official Full Name: phosphate-sensing transcription factor PHO4
Primary source: SGD:S000001930
Locus tag: YFR034C
See related: AllianceGenome:SGD:S000001930; FungiDB:YFR034C; VEuPathDB:YFR034C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA-binding transcription factor activity and sequence-specific DNA binding activity. Involved in several processes, including cellular response to phosphate starvation; positive regulation of phosphate metabolic process; and regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PHO4 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 1

Sequence:: Chromosome: VI; NC_001138.5 (225020..225958, complement)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Reb1, Cbf1, and Pho4 Bias Histone Sliding and Deposition Away from Their Binding Sites.
Title: Reb1, Cbf1, and Pho4 Bias Histone Sliding and Deposition Away from Their Binding Sites.
Pho85 and Pho4 regulated CLN2 expression under alkaline condition.
Title: The regulators of yeast PHO system participate in the transcriptional regulation of G1 cyclin under alkaline stress conditions.
Subsequent in-depth analysis of the inducible PHO5 gene reveals that topoisomerases are essential for binding of the Pho4p transcription factor to the PHO5 promoter, which is required for promoter nucleosome removal during activation.
Title: DNA Topoisomerases maintain promoters in a state competent for transcriptional activation in Saccharomyces cerevisiae.
The authors postulate that RPL12 has an extra-ribosomal function in modulating the transcription of genes that need Pho4p activation.
Title: Rpl12p affects the transcription of the PHO pathway high-affinity inorganic phosphate transporters and repressible phosphatases.
Pho4 activates some stress response genes, is required for G1 arrest caused by DNA damage, and is present in the nucleus even under high-Pi conditions to activate or repress transcription.
Title: Nutrient-regulated antisense and intragenic RNAs modulate a signal transduction pathway in yeast.
Pho4 binding to the SNZ1/SNO1 promoter is accompanied by alterations in chromatin structure, and Rpd3 histone deacetylase is required for the proper timing of SNZ1/SNO1 expression, while Asf1 histone chaperone is indispensable for their expression.
Title: Transcriptional repression by the Pho4 transcription factor controls the timing of SNZ1 expression.
three transcriptional outputs can be generated by a pathway whose regulation is controlled by one kinase, Pho80-Pho85, and one transcription factor, Pho4
Title: Partially phosphorylated Pho4 activates transcription of a subset of phosphate-responsive genes.
These data establish a G1 checkpoint adaptation pathway where Pho85 mediates Pho4 downregulation and Sic1 degradation to release Cdc28 activity and promote onset of S phase.
Title: CDK Pho85 targets CDK inhibitor Sic1 to relieve yeast G1 checkpoint arrest after DNA damage.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	HDA more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to phosphate starvation	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to phosphate starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to phosphate starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of phosphate metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: phosphate-sensing transcription factor PHO4

NP_116692.1

Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001138.5 Reference assembly

Range

225020..225958 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179999.1 → NP_116692.1 TPA: phosphate-sensing transcription factor PHO4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_116692.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTR7, P07270

UniProtKB/TrEMBL

A0A5P9VPY8, A7A274, B3LUP5, N1P6U5

Conserved Domains (1) summary

cd11392
Location:251 → 312

bHLH_ScPHO4_like; basic helix-loop-helix (bHLH) domain found in Saccharomyces cerevisiae phosphate system positive regulatory protein PHO4 and similar proteins