RSP5 NEDD4 family E3 ubiquitin-protein ligase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RSP5
Official Full Name: NEDD4 family E3 ubiquitin-protein ligase
Primary source: SGD:S000000927
Locus tag: YER125W
See related: AllianceGenome:SGD:S000000927; FungiDB:YER125W; VEuPathDB:YER125W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HPG1; MDP1; MUT2; NPI1; SMM1; UBY1
Summary: Enables phosphatidylinositol binding activity; ubiquitin binding activity; and ubiquitin-ubiquitin ligase activity. Involved in several processes, including proteolysis involved in protein catabolic process; regulation of lipid biosynthetic process; and regulation of ribonucleoprotein complex localization. Located in several cellular components, including Golgi apparatus; cellular bud tip; and endosome membrane. Part of ubiquitin ligase complex. Is active in cytosolic ribosome. Used to study Liddle syndrome. Human ortholog(s) of this gene implicated in periventricular nodular heterotopia. Orthologous to human NEDD4 (NEDD4 E3 ubiquitin protein ligase); NEDD4L (NEDD4 like E3 ubiquitin protein ligase); and WWP2 (WW domain containing E3 ubiquitin protein ligase 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RSP5 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (410189..412618)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transporters.
Title: A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transporters.
Regulation of the endocytosis and prion-chaperoning machineries by yeast E3 ubiquitin ligase Rsp5 as revealed by orthogonal ubiquitin transfer.
Title: Regulation of the endocytosis and prion-chaperoning machineries by yeast E3 ubiquitin ligase Rsp5 as revealed by orthogonal ubiquitin transfer.
The C2 domain of the ubiquitin ligase Rsp5 is required for ubiquitination of the endocytic protein Rvs167 upon change of nitrogen source.
Title: The C2 domain of the ubiquitin ligase Rsp5 is required for ubiquitination of the endocytic protein Rvs167 upon change of nitrogen source.
Downregulation of the broad-specificity amino acid permease Agp1 mediated by the ubiquitin ligase Rsp5 and the arrestin-like protein Bul1 in yeast.
Title: Downregulation of the broad-specificity amino acid permease Agp1 mediated by the ubiquitin ligase Rsp5 and the arrestin-like protein Bul1 in yeast.
A Cycle of Ubiquitination Regulates Adaptor Function of the Nedd4-Family Ubiquitin Ligase Rsp5.
Title: A Cycle of Ubiquitination Regulates Adaptor Function of the Nedd4-Family Ubiquitin Ligase Rsp5.
Rsp5 and Mdm30 reshape the mitochondrial network in response to age-induced vacuole stress in Saccharomyces cerevisiae.
Title: Rsp5 and Mdm30 reshape the mitochondrial network in response to age-induced vacuole stress.
Endocytic degradation of Acr3 is promoted by the ubiquitin ligase Rsp5 and requires polyubiquitination of Acr3 at multiple lysine residues via lysine 63-linked ubiquitin chains.
Title: Rsp5-dependent endocytosis and degradation of the arsenite transporter Acr3 requires its N-terminal acidic tail as an endocytic sorting signal and arrestin-related ubiquitin-ligase adaptors.
Ssh4-Rsp5 quality control system clears membrane proteins mistargeted to the vacuole membrane.
Title: Rsp5 Ubiquitin ligase-mediated quality control system clears membrane proteins mistargeted to the vacuole membrane.
Mdm30-Ubp2-Rsp5 crosstalk regulates mitochondrial fusion by coordinating an intricate balance between Fzo1 turnover and the status of fatty acids saturation.
Title: An ubiquitin-dependent balance between mitofusin turnover and fatty acids desaturation regulates mitochondrial fusion.
Ubp2 and Ubp3 deubiquitinases are required for the proteasomal degradation of cytosolic misfolded proteins targeted by Rsp5 after heat-shock
Title: Deubiquitinase activity is required for the proteasomal degradation of misfolded cytosolic proteins upon heat-stress.

Submit: New GeneRIF Correction See all GeneRIFs (67)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylinositol binding	IDA Inferred from Direct Assay more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin-ubiquitin ligase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to L-arginine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to amino acid stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in free ubiquitin chain polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in late endosome to vacuole transport via multivesicular body sorting pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in late endosome to vacuole transport via multivesicular body sorting pathway	IPI Inferred from Physical Interaction more info	PubMed
involved_in mitochondria-associated ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrion organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitochondrion organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nonfunctional rRNA decay	IDA Inferred from Direct Assay more info	PubMed
involved_in poly(A)+ mRNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of fatty acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of receptor-mediated endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IPI Inferred from Physical Interaction more info	PubMed
involved_in protein K63-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein quality control for misfolded or incompletely synthesized proteins	IDA Inferred from Direct Assay more info	PubMed
involved_in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin cytoskeleton organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of dolichol biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of dolichol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of ergosterol biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of ergosterol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mRNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mRNA export from nucleus	IPI Inferred from Physical Interaction more info	PubMed
involved_in regulation of nitrogen utilization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of phosphate metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization	IPI Inferred from Physical Interaction more info	PubMed
involved_in regulation of rRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of ribosomal large subunit export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of tRNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of tRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of ubiquinone biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of ubiquinone biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ubiquitin-dependent endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in actin cortical patch	IEA Inferred from Electronic Annotation more info
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in cytosolic ribosome	IDA Inferred from Direct Assay more info	PubMed
located_in endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	HDA more info	PubMed
part_of ubiquitin ligase complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: NEDD4 family E3 ubiquitin-protein ligase

NP_011051.3

EC 2.3.2.26

NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p, Sem1p, Dip5p and Put4p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

410189..412618

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179015.3 → NP_011051.3 TPA: NEDD4 family E3 ubiquitin-protein ligase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011051.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM31, P39940

UniProtKB/TrEMBL

B3LRP1, C7GKX2, G2WD00, N1P3J9

Conserved Domains (1) summary

COG5021
Location:3 → 809

HUL4; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]