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    HAS1 ATP-dependent RNA helicase HAS1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855335, updated on 9-Dec-2024

    Summary

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    Official Symbol
    HAS1
    Official Full Name
    ATP-dependent RNA helicase HAS1
    Primary source
    SGD:S000004903
    Locus tag
    YMR290C
    See related
    AllianceGenome:SGD:S000004903; FungiDB:YMR290C; VEuPathDB:YMR290C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables RNA binding activity and RNA helicase activity. Involved in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); and snoRNA release from pre-rRNA. Located in nuclear envelope and nucleolus. Part of preribosome, large subunit precursor. Orthologous to human DDX18 (DEAD-box helicase 18). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See HAS1 in Genome Data Viewer
    Location:
    chromosome: XIII
    Exon count:
    1
    Sequence:
    Chromosome: XIII; NC_001145.3 (850074..851591, complement)

    Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene U2 snRNP complex subunit HSH155 Neighboring gene aminodeoxychorismate lyase ABZ2 Neighboring gene protein kinase TDA1 Neighboring gene Got1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001145.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Characterization of the ATPase and unwinding activities of the yeast DEAD-box protein Has1p and the analysis of the roles of the conserved motifs. Rocak S, et al. Nucleic Acids Res, 2005. PMID 15718299, Free PMC Article
    2. Has1p, a member of the DEAD-box family, is required for 40S ribosomal subunit biogenesis in Saccharomyces cerevisiae. Emery B, et al. Mol Microbiol, 2004 Apr. PMID 15049817
    3. The dynamic three-dimensional organization of the diploid yeast genome. Kim S, et al. Elife, 2017 May 24. PMID 28537556, Free PMC Article
    4. Function of the C-terminal domain of the DEAD-box protein Mss116p analyzed in vivo and in vitro. Mohr G, et al. J Mol Biol, 2008 Feb 1. PMID 18096186, Free PMC Article
    5. Studies of the expression of human poly(ADP-ribose) polymerase-1 in Saccharomyces cerevisiae and identification of PARP-1 substrates by yeast proteome microarray screening. Tao Z, et al. Biochemistry, 2009 Dec 15. PMID 19877712

    See all (146) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. roles in triggering release of the U14 snoRNP, a critical event during early 40S maturation, and in driving assembly of domain I of pre-60S complexes
      Title: RNA helicases mediate structural transitions and compositional changes in pre-ribosomal complexes.
    2. The data supports a model in which Has1 helps to establish domain I architecture of 5.8S/25S rRNA to prevent pre-rRNA turnover and couples domain I folding with consecutive pre-rRNA processing steps.
      Title: Has1 regulates consecutive maturation and processing steps for assembly of 60S ribosomal subunits.
    3. Mutational analysis of the conserved residues in motifs that are important for ATPase and unwinding activities of Has1p.
      Title: Characterization of the ATPase and unwinding activities of the yeast DEAD-box protein Has1p and the analysis of the roles of the conserved motifs.
    4. Has1p helicase is required for snoRNA release from pre-rRNA and production of the U6 snRNP
      Title: The helicase Has1p is required for snoRNA release from pre-rRNA.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H2AZ histone chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H3-H4 histone complex chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent activity, acting on RNA IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA clamp loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chromatin extrusion motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cohesin loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin looping IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosomal large subunit biogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosomal small subunit biogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosome biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in snoRNA release from pre-rRNA IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of 90S preribosome HDA PubMed 
    located_in nuclear envelope IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleolus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of preribosome, large subunit precursor HDA PubMed 
    part_of preribosome, large subunit precursor IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ATP-dependent RNA helicase HAS1
    NP_014017.1
    • ATP-dependent RNA helicase; involved in the biogenesis of 40S and 60S ribosome subunits; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001145.3 Reference assembly

      Range
      850074..851591 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182797.1 → NP_014017.1  TPA: ATP-dependent RNA helicase HAS1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014017.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W0B7, Q03532
      UniProtKB/TrEMBL
      A6ZN06, B3LMH8, B5VQ58, C7GTK9, C8ZFF7, G2WKZ9, N1NYU8
      Conserved Domains (2) summary
      COG0513
      Location:16 → 475
      SrmB; Superfamily II DNA and RNA helicase [Replication, recombination and repair]
      cd17942
      Location:53 → 251
      DEADc_DDX18; DEAD-box helicase domain of DEAD box protein 18
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q03532.1

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