TSA1 thioredoxin peroxidase TSA1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TSA1
Official Full Name: thioredoxin peroxidase TSA1
Primary source: SGD:S000004490
Locus tag: YML028W
See related: AllianceGenome:SGD:S000004490; FungiDB:YML028W; VEuPathDB:YML028W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TPX1; ZRG14
Summary: Enables several functions, including ribosome binding activity; thioredoxin peroxidase activity; and unfolded protein binding activity. Involved in several processes, including DNA protection; cell redox homeostasis; and cellular response to dithiothreitol. Located in cytosol. Human ortholog(s) of this gene implicated in autosomal recessive spinocerebellar ataxia 32 and methylmalonic aciduria and homocystinuria type cblC. Orthologous to several human genes including PRDX1 (peroxiredoxin 1); PRDX2 (peroxiredoxin 2); and PRDX4 (peroxiredoxin 4). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See TSA1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (220138..220728)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Peroxiredoxin promotes longevity and H2O2-resistance in yeast through redox-modulation of protein kinase A.
Title: Peroxiredoxin promotes longevity and H(2)O(2)-resistance in yeast through redox-modulation of protein kinase A.
Deciphering the in vivo redox behavior of human peroxiredoxins I and II by expressing in budding yeast.
Title: Deciphering the in vivo redox behavior of human peroxiredoxins I and II by expressing in budding yeast.
It findings indicate that multiple steps in the catalytic cycle of Tsa1 may be impaired by introducing substitutions at its decamer-building interface, suggesting a multifaceted biological basis for its assembly into decamers.
Title: Aromatic Residues at the Dimer-Dimer Interface in the Peroxiredoxin Tsa1 Facilitate Decamer Formation and Biological Function.
Study found that Tsa1 and Hsp70 physically interact and that hyperoxidation of Tsa1 by hydrogen peroxide is required for the recruitment of the Hsp70 chaperones and the Hsp104 disaggregase to misfolded and aggregated proteins during aging, but not heat stress.
Title: Lifespan Control by Redox-Dependent Recruitment of Chaperones to Misfolded Proteins.
The critical molecular event allowing the Tsa1 to chaperone switch is the enzyme assembly into high molecular weight structures brought about by enzyme hyperoxidation and sulfinylation.
Title: In vivo parameters influencing 2-Cys Prx oligomerization: The role of enzyme sulfinylation.
Tsa1 localises to the sites of protein aggregation and protects against protein-aggregate-induced oxidative stress.
Title: The yeast peroxiredoxin Tsa1 protects against protein-aggregate-induced oxidative stress.
It appears to control the cellular reactive oxygen species.
Title: Requirement of peroxiredoxin on the stationary phase of yeast cell growth.
Tsa1, the major peroxiredoxin, is required for telomere length maintenance.
Title: Deletion of the major peroxiredoxin Tsa1 alters telomere length homeostasis.
These data collectively emphasize the significance of sulfiredoxin, peroxiredoxin, and mitochondrial respiratory chain to respond to and to protect from 100% O(2).
Title: Significance of sulfiredoxin/peroxiredoxin and mitochondrial respiratory chain in response to and protection from 100% O(2) in Saccharomyces cerevisiae.
Glu50 and Arg146 are important for the Tsa1-Trx interaction. These results may impact the comprehension of downstream events of signaling pathways that are triggered by the oxidation of critical Cys residues, such as Trx
Title: Disulfide biochemistry in 2-cys peroxiredoxin: requirement of Glu50 and Arg146 for the reduction of yeast Tsa1 by thioredoxin.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables kinase regulator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables peroxidase activity	IEA Inferred from Electronic Annotation more info
enables peroxiredoxin activity	IDA Inferred from Direct Assay more info	PubMed
enables peroxiredoxin activity	IEA Inferred from Electronic Annotation more info
enables ribosome binding	IDA Inferred from Direct Assay more info	PubMed
enables thioredoxin peroxidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables thioredoxin peroxidase activity	IDA Inferred from Direct Assay more info	PubMed
enables thioredoxin peroxidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables thioredoxin-dependent peroxiredoxin activity	IEA Inferred from Electronic Annotation more info
enables unfolded protein binding	IDA Inferred from Direct Assay more info	PubMed
enables unfolded protein binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA damage checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA protection	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell redox homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell redox homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in cell redox homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular oxidant detoxification	IEA Inferred from Electronic Annotation more info
involved_in cellular response to dithiothreitol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to heat	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to oxidative stress	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to oxidative stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chaperone-mediated protein folding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hydrogen peroxide catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein folding	IDA Inferred from Direct Assay more info	PubMed
involved_in protein folding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of gluconeogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to hydroperoxide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to oxidative stress	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to oxidative stress	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: thioredoxin peroxidase TSA1

NP_013684.1

EC 1.11.1.24

Thioredoxin peroxidase; acts as both ribosome-associated and free cytoplasmic antioxidant; self-associates to form a HMW chaperone complex under oxidative stress; chaperone activity essential for growth in zinc deficiency; hydrogen peroxide receptor and signal transducer in a light sensing pathway; required for telomere length maintenance; binds and modulates Cdc19p activity; protein abundance increases and forms cytoplasmic foci during DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

220138..220728

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182386.1 → NP_013684.1 TPA: thioredoxin peroxidase TSA1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013684.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZE6, P34760

UniProtKB/TrEMBL

A6ZM34, B3LLM4, C7GT58, C8ZEH6, G2WK24, N1NY02

Conserved Domains (1) summary

cd03015
Location:5 → 176

PRX_Typ2cys; Peroxiredoxin (PRX) family, Typical 2-Cys PRX subfamily; PRXs are thiol-specific antioxidant (TSA) proteins, which confer a protective role in cells through its peroxidase activity by reducing hydrogen peroxide, peroxynitrite, and organic hydroperoxides. ...