rdx roadkill [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: rdxprovided by FlyBase
Official Full Name: roadkillprovided by FlyBase
Primary source: FLYBASE:FBgn0264493
Locus tag: Dmel_CG12537
See related: AllianceGenome:FB:FBgn0264493
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 0869/09; anon-WO0118547.577; BEST:GM07940; CG10235; CG12537; CG9924; D-SPOP; Dmel\CG12537; Dromel_CG9924_FBtr0082871_rdx_mORF; hib; Hib; HIB; l(3)03477; l(3)2004; l(3)88Aa; l(3)A6; l(3)dsl-6; l(3)dsl6; l(3)j1D1; l(3)j4C7; l(3)S086909; l(3)SG40; mei-P19; Rdx; Spop; SPOP
Old locus tag: CG9924; Dmel_CG9924
Summary: Enables protein homodimerization activity and ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including negative regulation of protein import into nucleus; regulation of protein metabolic process; and regulation of signal transduction. Located in nucleus. Part of Cul3-RING ubiquitin ligase complex. Is expressed in several structures, including anlage in statu nascendi; anterior-posterior compartment boundary of imaginal disc; cuboidal/columnar epithelium; gut section; and head epidermis primordium. Human ortholog(s) of this gene implicated in colorectal cancer; hepatocellular carcinoma; and lung adenocarcinoma. Orthologous to human SPOP (speckle type BTB/POZ protein) and SPOPL (speckle type BTB/POZ protein like). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 88A3-88A4; 3-54 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (13967061..14032057, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (9792783..9857779, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Drosophila Hedgehog signaling promotes downregulation of Su(fu) through its target protein HIB (Hh-induced BTB protein).
Title: Hedgehog signaling downregulates suppressor of fused through the HIB/SPOP-Crn axis in Drosophila.
Dzip1-dependent stabilization of Spop/HIB is evolutionarily conserved and essential for proper regulation of Gli/Ci proteins in the Hh pathway.
Title: Stabilization of speckle-type POZ protein (Spop) by Daz interacting protein 1 (Dzip1) is essential for Gli turnover and the proper output of Hedgehog signaling.
Rdx blocks entry of Ci-155 into the nucleus in the absence of Su(fu) and Rdx induces the degradation of Ci-155 in the nucleus in the presence of a threshold level of Su(fu)
Title: Su(fu) switches Rdx functions to fine-tune hedgehog signaling in the Drosophila wing disk.
two different mechanisms by which Rdx negatively regulates Ci may play an important role in the fine-tuning of Hh responses
Title: Inhibition of the nuclear import of cubitus interruptus by roadkill in the presence of strong hedgehog signal.
a new role for SPOP (rdx) was identified; gene encoding SPOP is a target of segmentation transcription factors; SPOP mediates degradation of Puckered, thereby inducing tumor necrosis factor (TNF)/Eiger-dependent apoptosis
Title: Analysis of Drosophila segmentation network identifies a JNK pathway factor overexpressed in kidney cancer.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: c14023463-14021702 mobile_element_type = transposon:S{}rdx[S1357]

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin-like ligase-substrate adaptor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in eye development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein import into nucleus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of smoothened signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of JNK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of apoptotic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K48-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of proteolysis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in segment polarity determination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Cul3-RING ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: roadkill

Names: CG12537-PA; CG12537-PB; CG12537-PC; CG12537-PE; CG12537-PF; Cul3-Hedghog-induced MATH and BTB domain-containing protein; Hh-induced MATH and BTB domain containing protein; hedegehog-induced BTB protein; hedgehog- induced BTB protein; lethal (3) S086909; meiotic P19; rdx-PA; rdx-PB; rdx-PC; rdx-PE; rdx-PF

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

13967061..14032057 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_169564.2 → NP_731875.2 roadkill, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_731875.2

Status: REVIEWED

UniProtKB/TrEMBL

C7LAF6

Conserved Domains (3) summary

cd18345
Location:214 → 334

BTB_POZ_roadkill-like; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Drosophila melanogaster protein roadkill and similar proteins

cd18520
Location:329 → 402

BACK_roadkill_like; BACK (BTB and C-terminal Kelch) domain found in Drosophila melanogaster protein roadkill and similar proteins

cl02446
Location:60 → 198

MATH; MATH (meprin and TRAF-C homology) domain; an independent folding unit with an eight-stranded beta-sandwich structure found in meprins, TRAFs and other proteins. Meprins comprise a class of extracellular metalloproteases which are anchored to the membrane ...
NM_142068.2 → NP_650325.1 roadkill, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_650325.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9VFP2

Related

FBpp0290848

Conserved Domains (3) summary

cd18345
Location:211 → 331

BTB_POZ_roadkill-like; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Drosophila melanogaster protein roadkill and similar proteins

cd18520
Location:326 → 399

BACK_roadkill_like; BACK (BTB and C-terminal Kelch) domain found in Drosophila melanogaster protein roadkill and similar proteins

cl02446
Location:57 → 195

MATH; MATH (meprin and TRAF-C homology) domain; an independent folding unit with an eight-stranded beta-sandwich structure found in meprins, TRAFs and other proteins. Meprins comprise a class of extracellular metalloproteases which are anchored to the membrane ...
NM_142069.4 → NP_650326.3 roadkill, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_650326.3

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V2V6, Q15BT8, Q5BIA3, Q7KSK6, Q8ING4, Q8MRB4, Q9VFP2, Q9VFP3

Conserved Domains (4) summary

smart00225
Location:656 → 752

BTB; Broad-Complex, Tramtrack and Bric a brac

cd03774
Location:483 → 621

MATH_SPOP; Speckle-type POZ protein (SPOP) family, MATH domain; composed of proteins with similarity to human SPOP. SPOP was isolated as a novel antigen recognized by serum from a scleroderma patient, whose overexpression in COS cells results in a discrete speckled ...

cd14734
Location:752 → 814

SPOP_C; BACK-like Domain C-terminal to BTB in speckle-type POZ protein (SPOP) and similar proteins

pfam00651
Location:645 → 749

BTB; BTB/POZ domain
NM_169565.2 → NP_731876.2 roadkill, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_731876.2

Status: REVIEWED

UniProtKB/TrEMBL

C7LAF6

Related

FBpp0290846

Conserved Domains (3) summary

cd18345
Location:214 → 334

BTB_POZ_roadkill-like; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Drosophila melanogaster protein roadkill and similar proteins

cd18520
Location:329 → 402

BACK_roadkill_like; BACK (BTB and C-terminal Kelch) domain found in Drosophila melanogaster protein roadkill and similar proteins

cl02446
Location:60 → 198

MATH; MATH (meprin and TRAF-C homology) domain; an independent folding unit with an eight-stranded beta-sandwich structure found in meprins, TRAFs and other proteins. Meprins comprise a class of extracellular metalloproteases which are anchored to the membrane ...
NM_169566.2 → NP_731877.1 roadkill, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_731877.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9VFP2

Related

FBpp0290847

Conserved Domains (3) summary

cd18345
Location:182 → 302

BTB_POZ_roadkill-like; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Drosophila melanogaster protein roadkill and similar proteins

cd18520
Location:297 → 370

BACK_roadkill_like; BACK (BTB and C-terminal Kelch) domain found in Drosophila melanogaster protein roadkill and similar proteins

cl02446
Location:28 → 166

MATH; MATH (meprin and TRAF-C homology) domain; an independent folding unit with an eight-stranded beta-sandwich structure found in meprins, TRAFs and other proteins. Meprins comprise a class of extracellular metalloproteases which are anchored to the membrane ...