AMPKalpha AMP-activated protein kinase alpha subunit [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: AMPKalphaprovided by FlyBase
Official Full Name: AMP-activated protein kinase alpha subunitprovided by FlyBase
Primary source: FLYBASE:FBgn0023169
Locus tag: Dmel_CG3051
See related: AllianceGenome:FB:FBgn0023169
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: AK; ampk; Ampk; AMPK; AMPK alpha; AMPK-alpha; ampkalpha; AmpKalpha; CG3051; dAMPK; dAMPKa; dAMPKalpha; DmAMPK alpha; Dmel\CG3051; EG:132E8.2; FBgn0023169; Gprk-4; Gprk4; snf1a; snf1A; SNF1A; SNF4A-a
Summary: Enables AMP-activated protein kinase activity. Involved in several processes, including behavioral response to starvation; negative regulation of cell size; and regulation of TORC1 signaling. Located in basolateral part of cell; endosome; and nucleus. Is expressed in adult head. Used to study Parkinson's disease and obesity. Human ortholog(s) of this gene implicated in Huntington's disease; breast cancer; colon cancer; and type 2 diabetes mellitus. Orthologous to several human genes including PRKAA2 (protein kinase AMP-activated catalytic subunit alpha 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 2A1-2A1; 1-0.2 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (1375536..1378896)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (1269569..1272929)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phosphorylation of LKB1 by PDK1 Inhibits Cell Proliferation and Organ Growth by Decreased Activation of AMPK.
Title: Phosphorylation of LKB1 by PDK1 Inhibits Cell Proliferation and Organ Growth by Decreased Activation of AMPK.
A conserved role for AMP-activated protein kinase in NGLY1 deficiency.
Title: A conserved role for AMP-activated protein kinase in NGLY1 deficiency.
Data (including data from transgenic flies) suggest that SNF1A is involved in neuroprotection in this species; AMPK activation in glia (but not neurons) reduces severity of ionic disruption and prolongs recovery of electrical activity in brains of flies in starvation-induced anoxia.
Title: AMP-activated protein kinase protects against anoxia in Drosophila melanogaster.
AMPK can function outside of its canonical nutrient-sensing role in specific developmental contexts.
Title: AMP-activated protein kinase has diet-dependent and -independent roles in Drosophila oogenesis.
The significance of these findings is that AMPK is important in the regulation of glucagon signaling, suggesting that the organization of metabolic networks is highly conserved and that AMPK plays a prominent role in these networks.
Title: Energy-dependent modulation of glucagon-like signaling in Drosophila via the AMP-activated protein kinase.
Loss of AMPK activity exacerbates neuronal loss and associated phenotypes in parkin and LRRK transgenic flies.
Title: AMP kinase activation mitigates dopaminergic dysfunction and mitochondrial abnormalities in Drosophila models of Parkinson's disease.
Data suggest that that an Sgt1/Hsp90-LKB1-AMPK pathway acts redundantly with a microtubule-induced polarity pathway to generate neuroblast cortical polarity, and the absence of neuroblast cortical polarity can produce neuroblast tumors.
Title: Sgt1 acts via an LKB1/AMPK pathway to establish cortical polarity in larval neuroblasts.
AMPK gamma and alpha mutants altered lipid metabolism and showed persistent starvation behaviors.
Title: Altered metabolism and persistent starvation behaviors caused by reduced AMPK function in Drosophila.
AMPK supports growth in Drosophila by regulating muscle activity and nutrient uptake in the gut
Title: AMPK supports growth in Drosophila by regulating muscle activity and nutrient uptake in the gut.
These results raise the possibility that muscle is one of major tissues in which AMPK plays a critical role on longevity and stress resistance.
Title: A critical role of SNF1A/dAMPKalpha (Drosophila AMP-activated protein kinase alpha) in muscle on longevity and stress resistance in Drosophila melanogaster.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables AMP-activated protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables AMP-activated protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in behavioral response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to glucose starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of epithelial cell apical/basal polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TOR signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of TORC1 signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell size	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of TORC1 signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of autophagosome assembly	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in positive regulation of axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of lipophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of macroautophagy	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of digestive system process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sequestering of triglyceride	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in basolateral part of cell	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
part_of nucleotide-activated protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of nucleotide-activated protein kinase complex	IC Inferred by Curator more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: AMP-activated protein kinase alpha subunit

Names: AMP activated kinase; AMP-activated kinase; AMP-activated protein kinase; AMP-dependent kinase; AMPK alpha subunit; AMPK catalytic subunit; AMPKalpha-PA; AMPKalpha-PB; AMPKalpha-PC; CG3051-PA; CG3051-PB; CG3051-PC; G protein-coupled receptor kinase 4; SNF1A/AMP-activated protein kinase; adenosine monophosphate-activated protein kinase

NP_477313.1

EC 2.7.11.1

EC 2.7.11.11

NP_726730.1

EC 2.7.11.1

EC 2.7.11.11

NP_996327.1

EC 2.7.11.1

EC 2.7.11.11

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

1375536..1378896

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_166880.2 → NP_726730.1 AMP-activated protein kinase alpha subunit, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726730.1

Status: REVIEWED

UniProtKB/TrEMBL

O18645, Q5U118, Q9U4G0

Related

FBpp0070209

Conserved Domains (3) summary

cd12122
Location:457 → 580

AMPKA_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha catalytic subunit

cd14336
Location:297 → 360

UBA_AID_AMPKalpha; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic alpha (AMPKalpha) subunits

cd14079
Location:25 → 280

STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase
NM_206604.2 → NP_996327.1 AMP-activated protein kinase alpha subunit, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996327.1

Status: REVIEWED

UniProtKB/TrEMBL

O18645, Q5U118, Q9U4G0

Conserved Domains (3) summary

cd12122
Location:457 → 580

AMPKA_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha catalytic subunit

cd14336
Location:297 → 360

UBA_AID_AMPKalpha; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic alpha (AMPKalpha) subunits

cd14079
Location:25 → 280

STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase
NM_057965.4 → NP_477313.1 AMP-activated protein kinase alpha subunit, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477313.1

Status: REVIEWED

UniProtKB/TrEMBL

O18645, Q5U118, Q9U4G0

Related

FBpp0070208

Conserved Domains (3) summary

cd12122
Location:457 → 580

AMPKA_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha catalytic subunit

cd14336
Location:297 → 360

UBA_AID_AMPKalpha; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic alpha (AMPKalpha) subunits

cd14079
Location:25 → 280

STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase