Dap160 Dynamin associated protein 160 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Dap160provided by FlyBase
Official Full Name: Dynamin associated protein 160provided by FlyBase
Primary source: FLYBASE:FBgn0023388
Locus tag: Dmel_CG1099
See related: AllianceGenome:FB:FBgn0023388
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG1099; DAP-160; dap160; DAP160; Dap160/intersectin; Dmel\CG1099; noncoding_4110; p160
Summary: Enables protein kinase activator activity. Involved in several processes, including negative regulation of Notch signaling pathway; positive regulation of neuroblast proliferation; and synaptic vesicle endocytosis. Located in apical cortex and synaptic vesicle. Colocalizes with presynaptic membrane. Is expressed in several structures, including adult heart; embryonic foregut sensory structure; embryonic head epidermis; ganglia; and head. Used to study intellectual disability. Orthologous to several human genes including ITSN2 (intersectin 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 39A3-39A4; 2-54 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (21134439..21142347, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (21134439..21142347, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dap160 is involved in the regulation of endocytosis and exocytosis in synapses.
Title: An Endocytic Scaffolding Protein together with Synapsin Regulates Synaptic Vesicle Clustering in the Drosophila Neuromuscular Junction.
Findings imply that restoring the level of any one of these genes dap160/itsn1, synj/synj1, and nla/dscr1 may reduce endocytic defects seen in DS.
Title: Upregulation of three Drosophila homologs of human chromosome 21 genes alters synaptic function: implications for Down syndrome.
Dap160 positively regulates aPKC activity and localization to promote neuroblast cell polarity and cell cycle progression.
Title: Dap160/intersectin binds and activates aPKC to regulate cell polarity and cell cycle progression.
Dap160 scaffolds both endocytic machinery and essential synaptic signaling systems to the periactive zone to coordinately control structural and functional synapse development.
Title: Dap160/intersectin scaffolds the periactive zone to achieve high-fidelity endocytosis and normal synaptic growth.
Dap160, like dynamin, is involved in synaptic vesicle retrieval at active and periactive zones.
Title: Dap160/intersectin acts as a stabilizing scaffold required for synaptic development and vesicle endocytosis.
Dap160 controls synaptic vesicle membrane retrieval and synapse development.
Title: Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables molecular adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in bulk synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in clathrin-dependent synaptic vesicle endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in clathrin-dependent synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endosomal transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of Notch signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of neuroblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical cortex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in intracellular vesicle	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: dynamin associated protein 160

Names: CG1099-PA; CG1099-PB; CG1099-PC; CG1099-PD; CG1099-PG; CG1099-PH; Dap160-PA; Dap160-PB; Dap160-PC; Dap160-PD; Dap160-PG; Dap160-PH; Dynamin-associated protein 160 kDa; intersectin

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

21134439..21142347 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001259189.2 → NP_001246118.1 dynamin associated protein 160, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246118.1

Status: REVIEWED

UniProtKB/TrEMBL

M9ND00, O61618

Conserved Domains (10) summary

smart00027
Location:184 → 279

EH; Eps15 homology domain

cd11836
Location:674 → 728

SH3_Intersectin_1; First Src homology 3 domain (or SH3A) of Intersectin

cd11838
Location:779 → 830

SH3_Intersectin_3; Third Src homology 3 domain (or SH3C) of Intersectin

cd11839
Location:941 → 998

SH3_Intersectin_4; Fourth Src homology 3 domain (or SH3D) of Intersectin

cd11840
Location:1025 → 1077

SH3_Intersectin_5; Fifth Src homology 3 domain (or SH3E) of Intersectin

cd00052
Location:195 → 261

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

pfam05010
Location:369 → 570

TACC; Transforming acidic coiled-coil-containing protein (TACC)

pfam07106
Location:495 → 589

TBPIP; Tat binding protein 1(TBP-1)-interacting protein (TBPIP)

cd16269
Location:432 → 443

GBP_C; coiled coil [structural motif]

cl20817
Location:366 → 467

GBP_C; Guanylate-binding protein, C-terminal domain
NM_001273735.1 → NP_001260664.1 dynamin associated protein 160, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260664.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PDC7, O61639

Conserved Domains (10) summary

smart00027
Location:184 → 279

EH; Eps15 homology domain

cd11836
Location:678 → 732

SH3_Intersectin_1; First Src homology 3 domain (or SH3A) of Intersectin

cd11838
Location:783 → 834

SH3_Intersectin_3; Third Src homology 3 domain (or SH3C) of Intersectin

cd11839
Location:862 → 919

SH3_Intersectin_4; Fourth Src homology 3 domain (or SH3D) of Intersectin

cd11840
Location:946 → 998

SH3_Intersectin_5; Fifth Src homology 3 domain (or SH3E) of Intersectin

cd00052
Location:195 → 261

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

pfam05010
Location:373 → 574

TACC; Transforming acidic coiled-coil-containing protein (TACC)

pfam07106
Location:499 → 593

TBPIP; Tat binding protein 1(TBP-1)-interacting protein (TBPIP)

cd16269
Location:436 → 447

GBP_C; coiled coil [structural motif]

cl20817
Location:370 → 471

GBP_C; Guanylate-binding protein, C-terminal domain
NM_001273734.1 → NP_001260663.1 dynamin associated protein 160, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260663.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PBF6, O61618

Conserved Domains (10) summary

smart00027
Location:184 → 279

EH; Eps15 homology domain

cd11836
Location:678 → 732

SH3_Intersectin_1; First Src homology 3 domain (or SH3A) of Intersectin

cd11838
Location:783 → 834

SH3_Intersectin_3; Third Src homology 3 domain (or SH3C) of Intersectin

cd11839
Location:945 → 1002

SH3_Intersectin_4; Fourth Src homology 3 domain (or SH3D) of Intersectin

cd11840
Location:1029 → 1081

SH3_Intersectin_5; Fifth Src homology 3 domain (or SH3E) of Intersectin

cd00052
Location:195 → 261

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

pfam05010
Location:373 → 574

TACC; Transforming acidic coiled-coil-containing protein (TACC)

pfam07106
Location:499 → 593

TBPIP; Tat binding protein 1(TBP-1)-interacting protein (TBPIP)

cd16269
Location:436 → 447

GBP_C; coiled coil [structural motif]

cl20817
Location:370 → 471

GBP_C; Guanylate-binding protein, C-terminal domain
NM_057975.4 → NP_477323.1 dynamin associated protein 160, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477323.1

Status: REVIEWED

UniProtKB/TrEMBL

O61639, Q8INU2

Related

FBpp0081032

Conserved Domains (10) summary

smart00027
Location:184 → 279

EH; Eps15 homology domain

cd11836
Location:678 → 732

SH3_Intersectin_1; First Src homology 3 domain (or SH3A) of Intersectin

cd11838
Location:783 → 834

SH3_Intersectin_3; Third Src homology 3 domain (or SH3C) of Intersectin

cd11839
Location:862 → 919

SH3_Intersectin_4; Fourth Src homology 3 domain (or SH3D) of Intersectin

cd11840
Location:946 → 998

SH3_Intersectin_5; Fifth Src homology 3 domain (or SH3E) of Intersectin

cd00052
Location:195 → 261

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

pfam05010
Location:373 → 574

TACC; Transforming acidic coiled-coil-containing protein (TACC)

pfam07106
Location:499 → 593

TBPIP; Tat binding protein 1(TBP-1)-interacting protein (TBPIP)

cd16269
Location:436 → 447

GBP_C; coiled coil [structural motif]

cl20817
Location:370 → 471

GBP_C; Guanylate-binding protein, C-terminal domain
NM_001169557.2 → NP_001163028.1 dynamin associated protein 160, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001163028.1

Status: REVIEWED

UniProtKB/TrEMBL

O61618, Q1WWC9

Conserved Domains (10) summary

smart00027
Location:184 → 279

EH; Eps15 homology domain

cd11836
Location:678 → 732

SH3_Intersectin_1; First Src homology 3 domain (or SH3A) of Intersectin

cd11838
Location:783 → 834

SH3_Intersectin_3; Third Src homology 3 domain (or SH3C) of Intersectin

cd11839
Location:944 → 1001

SH3_Intersectin_4; Fourth Src homology 3 domain (or SH3D) of Intersectin

cd11840
Location:1028 → 1080

SH3_Intersectin_5; Fifth Src homology 3 domain (or SH3E) of Intersectin

cd00052
Location:195 → 261

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

pfam05010
Location:373 → 574

TACC; Transforming acidic coiled-coil-containing protein (TACC)

pfam07106
Location:499 → 593

TBPIP; Tat binding protein 1(TBP-1)-interacting protein (TBPIP)

cd16269
Location:436 → 447

GBP_C; coiled coil [structural motif]

cl20817
Location:370 → 471

GBP_C; Guanylate-binding protein, C-terminal domain
NM_057974.4 → NP_477322.1 dynamin associated protein 160, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477322.1

Status: REVIEWED

UniProtKB/TrEMBL

O61618, Q9VIF7

Related

FBpp0081031

Conserved Domains (10) summary

smart00027
Location:184 → 279

EH; Eps15 homology domain

cd11836
Location:678 → 732

SH3_Intersectin_1; First Src homology 3 domain (or SH3A) of Intersectin

cd11838
Location:783 → 834

SH3_Intersectin_3; Third Src homology 3 domain (or SH3C) of Intersectin

cd11839
Location:945 → 1002

SH3_Intersectin_4; Fourth Src homology 3 domain (or SH3D) of Intersectin

cd11840
Location:1029 → 1081

SH3_Intersectin_5; Fifth Src homology 3 domain (or SH3E) of Intersectin

cd00052
Location:195 → 261

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

pfam05010
Location:373 → 574

TACC; Transforming acidic coiled-coil-containing protein (TACC)

pfam07106
Location:499 → 593

TBPIP; Tat binding protein 1(TBP-1)-interacting protein (TBPIP)

cd16269
Location:436 → 447

GBP_C; coiled coil [structural motif]

cl20817
Location:370 → 471

GBP_C; Guanylate-binding protein, C-terminal domain

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
M9ND00		M9ND00
M9PBF6		M9PBF6
M9PDC7		M9PDC7
O61618		O61618
O61639		O61639
Q1WWC9		Q1WWC9
Q8INU2		Q8INU2
Q8T068		Q8T068
Q9VIF7		Q9VIF7