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    Nsf2 N-ethylmaleimide-sensitive factor 2 [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 41694, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Nsf2provided by FlyBase
    Official Full Name
    N-ethylmaleimide-sensitive factor 2provided by FlyBase
    Primary source
    FLYBASE:FBgn0266464
    Locus tag
    Dmel_CG33101
    See related
    AllianceGenome:FB:FBgn0266464
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG33101; CG9931; Dmel\CG33101; DmNSF2; dNSF; dNSF-2; dnsf2; dNsf2; dNSF2; EC3-11; lotus; Nsf-2; NSF-2; NSF2; urd
    Summary
    Predicted to enable ATP hydrolysis activity. Involved in neuromuscular synaptic transmission; regulation of synaptic assembly at neuromuscular junction; and trachea morphogenesis. Predicted to be located in cytoplasm. Predicted to be active in Golgi stack. Is expressed in several structures, including adult head; embryonic/larval midgut primordium; germ layer; gut section; and imaginal disc. Used to study Parkinson's disease. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 96. Orthologous to human NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See Nsf2 in Genome Data Viewer
    Location:
    87F15-87F15; 3-54 cM
    Exon count:
    4
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (13833497..13837991, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3R NT_033777.2 (9659219..9663713, complement)

    Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene long non-coding RNA:CR43642 Neighboring gene long non-coding RNA:CR43300 Neighboring gene Male-specific RNA 87F Neighboring gene uncharacterized protein Neighboring gene pseudo Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Early consolidation of development and physiology of an identified presynaptic nerve terminal. Laviolette M, et al. BMC Neurosci, 2013 Oct 17. PMID 24134061, Free PMC Article
    2. Disruption of synaptic development and ultrastructure by Drosophila NSF2 alleles. Stewart BA, et al. J Comp Neurol, 2005 Jul 18. PMID 15912502
    3. Distinct roles for N-ethylmaleimide-sensitive fusion protein (NSF) suggested by the identification of a second Drosophila NSF homolog. Pallanck L, et al. J Biol Chem, 1995 Aug 11. PMID 7642522
    4. Moesin helps to restrain synaptic growth at the Drosophila neuromuscular junction. Seabrooke S, et al. Dev Neurobiol, 2008 Feb 15. PMID 18161855
    5. Identification of a second homolog of N-ethylmaleimide-sensitive fusion protein that is expressed in the nervous system and secretory tissues of Drosophila. Boulianne GL, et al. Proc Natl Acad Sci U S A, 1995 Jul 18. PMID 7624376, Free PMC Article

    See all (90) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. CCM3 and GCKIII prevent tube dilation in Drosophila trachea; tube dilation is suppressed by reduction of NSF2
      Title: Focal defects in single-celled tubes mutant for Cerebral cavernous malformation 3, GCKIII, or NSF2.
    2. Interaction of cytoskeleton genes with NSF2-induced neuromuscular junction overgrowth suggests that NSF may function near the top of the actin regulatory pathway.
      Title: Interaction of cytoskeleton genes with NSF2-induced neuromuscular junction overgrowth.
    3. NSF2(E/Q) larval synapses indicate the NMJ overgrowth phenotype is present at the embryonic neuromuscular synapse. At the ultrastructural level, considerable alterations in the number and distribution of synapses and active zones were found.
      Title: Disruption of synaptic development and ultrastructure by Drosophila NSF2 alleles.
    4. genetic analysis of suppressors of NSF2 neuromuscular junction overgrowth
      Title: A genetic screen for suppressors of Drosophila NSF2 neuromuscular junction overgrowth.
    5. These data are consistent with a model in which actin filaments promote vesicle mobility and suggest that N-ethylmaleimide sensitive factor participates in establishing or maintaining this population of actin.
      Title: Synaptic vesicle mobility and presynaptic F-actin are disrupted in a N-ethylmaleimide-sensitive factor allele of Drosophila.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Process Evidence Code Pubs
    involved_in Golgi to plasma membrane protein transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in SNARE complex disassembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in SNARE complex disassembly ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in intra-Golgi vesicle-mediated transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in liquid clearance, open tracheal system HMP PubMed 
    involved_in neuromuscular synaptic transmission IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in neurotransmitter secretion NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in open tracheal system development HMP PubMed 
    involved_in regulation of synaptic assembly at neuromuscular junction IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in synaptic vesicle priming NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in terminal branching, open tracheal system HMP PubMed 
    involved_in trachea morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in vesicle-mediated transport ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in Golgi stack IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in cytoplasm NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    N-ethylmaleimide-sensitive factor 2
    Names
    CG33101-PA
    CG33101-PB
    CG33101-PC
    N-ethylmaleimide sensitive factor 2
    NEM-sensitive fusion protein 2
    Nsf2-PA
    Nsf2-PB
    Nsf2-PC
    lotus

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033777.3 Reference assembly

      Range
      13833497..13837991 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001300390.1 → NP_001287319.1  N-ethylmaleimide-sensitive factor 2, isoform C [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001287319.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P54351, Q494J2, Q9VFQ5
      UniProtKB/TrEMBL
      A0A0B4LH53
      Conserved Domains (5) summary
      smart00382
      Location:259 → 404
      AAA; ATPases associated with a variety of cellular activities
      smart01072
      Location:117 → 170
      CDC48_2; Cell division protein 48 (CDC48) domain 2
      smart01073
      Location:9 → 89
      CDC48_N; Cell division protein 48 (CDC48) N-terminal domain
      pfam00004
      Location:261 → 402
      AAA; ATPase family associated with various cellular activities (AAA)
      cl21455
      Location:544 → 613
      P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

    2. NM_001300389.1 → NP_001287318.1  N-ethylmaleimide-sensitive factor 2, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001287318.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P54351, Q494J2, Q9VFQ5
      UniProtKB/TrEMBL
      A0A0B4LH53
      Conserved Domains (5) summary
      smart00382
      Location:259 → 404
      AAA; ATPases associated with a variety of cellular activities
      smart01072
      Location:117 → 170
      CDC48_2; Cell division protein 48 (CDC48) domain 2
      smart01073
      Location:9 → 89
      CDC48_N; Cell division protein 48 (CDC48) N-terminal domain
      pfam00004
      Location:261 → 402
      AAA; ATPase family associated with various cellular activities (AAA)
      cl21455
      Location:544 → 613
      P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

    3. NM_176499.2 → NP_788676.1  N-ethylmaleimide-sensitive factor 2, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_788676.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P54351, Q494J2, Q9VFQ5
      UniProtKB/TrEMBL
      A0A0B4LH53
      Related
      FBpp0082346
      Conserved Domains (5) summary
      smart00382
      Location:259 → 404
      AAA; ATPases associated with a variety of cellular activities
      smart01072
      Location:117 → 170
      CDC48_2; Cell division protein 48 (CDC48) domain 2
      smart01073
      Location:9 → 89
      CDC48_N; Cell division protein 48 (CDC48) N-terminal domain
      pfam00004
      Location:261 → 402
      AAA; ATPase family associated with various cellular activities (AAA)
      cl21455
      Location:544 → 613
      P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A0A0B4LH53 A0A0B4LH53
    P54351.2 P54351

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