dpn deadpan [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: dpnprovided by FlyBase
Official Full Name: deadpanprovided by FlyBase
Primary source: FLYBASE:FBgn0010109
Locus tag: Dmel_CG8704
See related: AllianceGenome:FB:FBgn0010109
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 44C; anon-EST:fe1B12; bHLHe50; CG8704; Deadpan; Dmel\CG8704; Dpn; DPN
Summary: Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and cis-regulatory region sequence-specific DNA binding activity. Involved in several processes, including generation of neurons; positive regulation of neuroblast proliferation; and regulation of gene expression. Located in cytosol and nucleus. Is expressed in several structures, including embryonic segment; head epidermis primordium; larval optic anlage; neuroblasts; and neurogenic region. Used to study brain cancer and central nervous system cancer. Orthologous to human HES1 (hes family bHLH transcription factor 1) and HES4 (hes family bHLH transcription factor 4). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 44C2-44C2; 2-58 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (8228971..8232330, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (4116476..4119835, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dissecting Hes-centred transcriptional networks in neural stem cell maintenance and tumorigenesis in Drosophila.
Title: Dissecting Hes-centred transcriptional networks in neural stem cell maintenance and tumorigenesis in Drosophila.
The tumor suppressor Brat controls neuronal stem cell lineages by inhibiting Deadpan and Zelda.
Title: The tumor suppressor Brat controls neuronal stem cell lineages by inhibiting Deadpan and Zelda.
N activity is required to promote dpn transcription; only in R7 photoreceptor precursors does the removal of Ttk coincide with high N activity; and only in this cell does Dpn expression result
Title: R7 Photoreceptor Specification in the Developing Drosophila Eye: The Role of the Transcription Factor Deadpan.
Dpn contributes to the establishment of Cut expression.
Title: Deadpan contributes to the robustness of the notch response.
Data suggest that Dpn plays important roles in maintaining NB self-renewal and specification of type II NBs in larval brains and that Dpn and Notch function independently in regulating type II NB proliferation and specification.
Title: The bHLH repressor Deadpan regulates the self-renewal and specification of Drosophila larval neural stem cells independently of Notch.
E(spl) and Dpn act together to maintain the neuroblasts in a self-renewing state, a process in which they are assisted by Notch, which sustains expression of the E(spl) subset
Title: bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and mediate Notch-induced overproliferation.
In addition to dpn, Notch signaling must be regulating other genes during this process that act redundantly with dpn.
Title: The bHLH factor deadpan is a direct target of Notch signaling and regulates neuroblast self-renewal in Drosophila.
Groucho and associated maternal and zygotic bHLH repressors define the threshold X-linked signal element gene concentrations needed to activate Sex-lethal establishment promoter and they participate directly in sex signal amplification.
Title: Maternal Groucho and bHLH repressors amplify the dose-sensitive X chromosome signal in Drosophila sex determination.
Dpn is a partner and target of CK2, and raises the possibility that its repressor functions might also be regulated by phosphorylation.
Title: Drosophila CK2 phosphorylates Deadpan, a member of the HES family of basic-helix-loop-helix (bHLH) repressors.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in R7 cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in adult locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in anterior/posterior pattern specification	IBA Inferred from Biological aspect of Ancestor more info
involved_in larval locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuroblast development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of neuroblast proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of neuroblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of neuroblast proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of neuroblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neurogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in sex determination, establishment of X:A ratio	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sex determination, establishment of X:A ratio	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sex-chromosome dosage compensation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: deadpan

Names: CG8704-PA; Dead-pan; anon-fast-evolving-1B12; dpn-PA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

8228971..8232330 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057575.3 → NP_476923.1 deadpan [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476923.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q26263, Q9V384

Related

FBpp0087879

Conserved Domains (2) summary

smart00511
Location:114 → 158

ORANGE; Orange domain

cd00083
Location:39 → 101

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...