Pi3K59F Phosphatidylinositol 3-kinase 59F [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Pi3K59Fprovided by FlyBase
Official Full Name: Phosphatidylinositol 3-kinase 59Fprovided by FlyBase
Primary source: FLYBASE:FBgn0015277
Locus tag: Dmel_CG5373
See related: AllianceGenome:FB:FBgn0015277
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG5373; Dmel\CG5373; DmVps34; dPI3K; dVps34; PI(3)K; Pi3K; PI3K; PI3K-59F; pi3k59f; Pi3k59F; PI3K59F; Pi3K59F (Vps34); Pi3K59F/Vps34; Pi3K_59F; PI3K_59F; PIK359F; PI[[3]]K; vps34; Vps34; VPS34
Summary: Enables 1-phosphatidylinositol-3-kinase activity. Involved in several processes, including dsRNA transport; larval midgut cell programmed cell death; and neuron remodeling. Located in autophagosome membrane; early endosome; and omegasome. Part of phosphatidylinositol 3-kinase complex, class III, type I. Is expressed in organism and spermatozoon. Orthologous to human PIK3C3 (phosphatidylinositol 3-kinase catalytic subunit type 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 59E4-59F1; 2-103 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (23560492..23564045)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (19448015..19451568)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

lowered expression of Atg6 or Pi3K59F in Ddc-Gal4-expressing neurons results in decreased longevity and associated age-dependent loss of locomotor ability. Inhibition of Atg6 or Pi3K59F together with overexpression of the sole pro-survival Bcl-2 Drosophila homolog Buffy in Ddc-Gal4-expressing neurons resulted in further decrease in the survival
Title: Inhibition of Atg6 and Pi3K59F autophagy genes in neurons decreases lifespan and locomotor ability in Drosophila melanogaster.
The UVRAG-containing PI3K (III) complex is required for receptor downregulation through endolysosomal degradation and for the establishment of proper cell polarity in the developing wing.
Title: Atg6/UVRAG/Vps34-containing lipid kinase complex is required for receptor downregulation through endolysosomal degradation and epithelial polarity during Drosophila wing development.
crystal structure of Vps34 at 2.9 A resolution, which revealed a constricted adenine-binding pocket; the phosphoinositide-binding loop & the carboxyl-terminal helix of Vps34 mediate catalysis on membranes & suppress futile adenosine triphosphatase cycles
Title: Shaping development of autophagy inhibitors with the structure of the lipid kinase Vps34.
Vps34 is regulated by TOR-dependent nutrient signals directly at sites of autophagosome formation.
Title: The class III PI(3)K Vps34 promotes autophagy and endocytosis but not TOR signaling in Drosophila.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables 1-phosphatidylinositol-3-kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 1-phosphatidylinositol-3-kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables 1-phosphatidylinositol-3-kinase activity	IEA Inferred from Electronic Annotation more info
enables 1-phosphatidylinositol-3-kinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
NOT enables 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity	IDA Inferred from Direct Assay more info	PubMed
NOT enables 1-phosphatidylinositol-4-phosphate 3-kinase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in autophagosome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epithelial structure maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment or maintenance of cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in larval midgut cell programmed cell death	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in macroautophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pexophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol phosphate biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylinositol phosphate biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylinositol phosphate biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in phosphatidylinositol-3-phosphate biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of nurse cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein targeting to lysosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of early endosome to late endosome transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in autophagosome membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in endosome	IBA Inferred from Biological aspect of Ancestor more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in omegasome	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in peroxisome	IBA Inferred from Biological aspect of Ancestor more info
is_active_in phagophore assembly site	IBA Inferred from Biological aspect of Ancestor more info
part_of phosphatidylinositol 3-kinase complex, class III, type I	IBA Inferred from Biological aspect of Ancestor more info
part_of phosphatidylinositol 3-kinase complex, class III, type I	IDA Inferred from Direct Assay more info	PubMed
part_of phosphatidylinositol 3-kinase complex, class III, type II	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: phosphatidylinositol 3-kinase 59F

Names: CG5373-PA; PI 3-kinase; PI3-kinase; Pi3K59F-PA; dPI 3-kinase; phosphotidylinositol 3 kinase 59F

NP_477133.1

EC 2.7.1.137

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

23560492..23564045

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057785.5 → NP_477133.1 phosphatidylinositol 3-kinase 59F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477133.1

Status: REVIEWED

UniProtKB/TrEMBL

P91635, Q95U22, Q9W1M7

Related

FBpp0071973

Conserved Domains (3) summary

cd08397
Location:27 → 186

C2_PI3K_class_III; C2 domain present in class III phosphatidylinositol 3-kinases (PI3Ks)

cd00870
Location:284 → 561

PI3Ka_III; Phosphoinositide 3-kinase (PI3K) class III, accessory domain (PIK domain); PIK domain is conserved in all PI3 and PI4-kinases. Its role is unclear but it has been suggested to be involved in substrate presentation. In general, PI3Ks class III ...

cd00896
Location:601 → 945

PI3Kc_III; Catalytic domain of Class III Phosphoinositide 3-kinase