Mfe2 peroxisomal Multifunctional enzyme type 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Mfe2provided by FlyBase
Official Full Name: peroxisomal Multifunctional enzyme type 2provided by FlyBase
Primary source: FLYBASE:FBgn0030731
Locus tag: Dmel_CG3415
See related: AllianceGenome:FB:FBgn0030731
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG3415; DBP; Dmel\CG3415; DmMFE-2; HSD17B4; MFE-2; MFE2
Summary: Enables 3-hydroxyacyl-CoA dehydratase activity and protein homodimerization activity. Predicted to be involved in fatty acid beta-oxidation. Predicted to be active in peroxisome. Is expressed in adult head and adult heart. Human ortholog(s) of this gene implicated in D-bifunctional protein deficiency; Perrault syndrome; and Stiff-Person syndrome. Orthologous to human HSD17B4 (hydroxysteroid 17-beta dehydrogenase 4). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 14B7-14B7; 1-53 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (16270951..16273815)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (16164984..16167848)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Molecular models of domain structure of MFE-2 from human, C. elegans, and Drosophila melanogaster lend support to possible structural role of MFE-2 domains including SCP-2L (sterol carrier protein 2-like) domain in human and C. elegans proteins.
Title: Quaternary structure of human, Drosophila melanogaster and Caenorhabditis elegans MFE-2 in solution from synchrotron small-angle X-ray scattering.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables 17-beta-hydroxysteroid dehydrogenase (NAD+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3-hydroxyacyl-CoA dehydratase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3-hydroxyacyl-CoA dehydrogenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3-hydroxyacyl-CoA dehydrogenase activity	ISS Inferred from Sequence or Structural Similarity more info
enables enoyl-CoA hydratase activity	IBA Inferred from Biological aspect of Ancestor more info
enables enoyl-CoA hydratase activity	IDA Inferred from Direct Assay more info	PubMed
enables estradiol 17-beta-dehydrogenase [NAD(P)+] activity	ISS Inferred from Sequence or Structural Similarity more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in fatty acid beta-oxidation	IBA Inferred from Biological aspect of Ancestor more info
involved_in fatty acid beta-oxidation using acyl-CoA oxidase	IC Inferred by Curator more info	PubMed

Component	Evidence Code	Pubs
is_active_in peroxisome	IBA Inferred from Biological aspect of Ancestor more info
located_in peroxisome	IC Inferred by Curator more info	PubMed
located_in peroxisome	ISM Inferred from Sequence Model more info	PubMed
located_in peroxisome	ISS Inferred from Sequence or Structural Similarity more info	PubMed

General protein information

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Preferred Names: peroxisomal multifunctional enzyme type 2

Names: CG3415-PA; CG3415-PB; Mfe2-PA; Mfe2-PB; multifunctional enzyme type 2

NP_001285318.1

EC 1.-.-.-

EC 1.1.1.35

EC 1.1.1.62

EC 4.2.1.17

EC 4.2.1.74

NP_573109.1

EC 1.-.-.-

EC 1.1.1.35

EC 1.1.1.62

EC 4.2.1.17

EC 4.2.1.74

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

16270951..16273815

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_132881.3 → NP_573109.1 peroxisomal multifunctional enzyme type 2, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_573109.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9VXJ0

UniProtKB/TrEMBL

X2JFD6

Related

FBpp0073988

Conserved Domains (5) summary

PLN02864
Location:315 → 598

PLN02864; enoyl-CoA hydratase

cd05353
Location:8 → 257

hydroxyacyl-CoA-like_DH_SDR_c-like; (3R)-hydroxyacyl-CoA dehydrogenase-like, classical(c)-like SDRs

PRK07791
Location:11 → 248

PRK07791; short chain dehydrogenase; Provisional

cd03448
Location:471 → 592

HDE_HSD; HDE_HSD The R-hydratase-like hot dog fold of the 17-beta-hydroxysteriod dehydrogenase (HSD), and Hydratase-Dehydrogenase-Epimerase (HDE) proteins. Other enzymes with this fold include MaoC dehydratase, and the fatty acid synthase beta subunit.

cl00509
Location:383 → 442

hot_dog; The hotdog fold was initially identified in the E. coli FabA (beta-hydroxydecanoyl-acyl carrier protein (ACP)-dehydratase) structure and subsequently in 4HBT (4-hydroxybenzoyl-CoA thioesterase) from Pseudomonas. A number of other seemingly unrelated ...
NM_001298389.1 → NP_001285318.1 peroxisomal multifunctional enzyme type 2, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285318.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9VXJ0

UniProtKB/TrEMBL

X2JFD6

Conserved Domains (5) summary

PLN02864
Location:315 → 598

PLN02864; enoyl-CoA hydratase

cd05353
Location:8 → 257

hydroxyacyl-CoA-like_DH_SDR_c-like; (3R)-hydroxyacyl-CoA dehydrogenase-like, classical(c)-like SDRs

PRK07791
Location:11 → 248

PRK07791; short chain dehydrogenase; Provisional

cd03448
Location:471 → 592

HDE_HSD; HDE_HSD The R-hydratase-like hot dog fold of the 17-beta-hydroxysteriod dehydrogenase (HSD), and Hydratase-Dehydrogenase-Epimerase (HDE) proteins. Other enzymes with this fold include MaoC dehydratase, and the fatty acid synthase beta subunit.

cl00509
Location:383 → 442

hot_dog; The hotdog fold was initially identified in the E. coli FabA (beta-hydroxydecanoyl-acyl carrier protein (ACP)-dehydratase) structure and subsequently in 4HBT (4-hydroxybenzoyl-CoA thioesterase) from Pseudomonas. A number of other seemingly unrelated ...