Eps15 epidermal growth factor receptor pathway substrate 15 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Eps15provided by MGI
Official Full Name: epidermal growth factor receptor pathway substrate 15provided by MGI
Primary source: MGI:MGI:104583
See related: Ensembl:ENSMUSG00000028552 AllianceGenome:MGI:104583
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 2410112D09Rik
Summary: Enables identical protein binding activity and ubiquitin binding activity. Involved in ubiquitin-dependent endocytosis. Acts upstream of or within regulation of protein localization. Located in ciliary membrane; clathrin-coated pit; and clathrin-coated vesicle. Part of AP-2 adaptor complex. Is expressed in several structures, including adrenal gland; alimentary system; heart; metanephros; and nervous system. Orthologous to human EPS15 (epidermal growth factor receptor pathway substrate 15). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in cortex adult (RPKM 26.3), cerebellum adult (RPKM 24.7) and 21 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 C7; 4 51.29 cM

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (109137372..109245014)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (109280175..109387817)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Liquid-like protein interactions catalyse assembly of endocytic vesicles.
Title: Liquid-like protein interactions catalyse assembly of endocytic vesicles.
Eps15 and Eps15L1 are required (redundantly) for correct embryo development; for the internalization of the transferrin receptor, which at the organismal level leads to microcytic hypochromic anemia when lacking in the hematopoietic system.
Title: Redundant and nonredundant organismal functions of EPS15 and EPS15L1.
Eps15 is a regulator of B-cell lymphopoiesis
Title: The endocytic adaptor Eps15 controls marginal zone B cell numbers.
Mouse hepatitis virus type 2 entry is mediated through clathrin-dependent but Eps15-independent endocytosis
Title: Mouse hepatitis virus type 2 enters cells through a clathrin-mediated endocytic pathway independent of Eps15.
a short amino-acid stretch at the carboxy-termini of the monoubiquitinated endocytic proteins Eps15 and eps15R is indispensable for their monoubiquitination
Title: A single motif responsible for ubiquitin recognition and monoubiquitination in endocytic proteins.
c-Cbl directs EGF receptors into an endocytic pathway that involves the ubiquitin-interacting motif of Eps15
Title: c-Cbl directs EGF receptors into an endocytic pathway that involves the ubiquitin-interacting motif of Eps15.
Our results suggest that spartin might be involved in endocytosis, vesicle trafficking, or mitogenic activity, and that impairment in one of these processes may underlie the long axonopathy in patients with Troyer syndrome.
Title: The Troyer syndrome (SPG20) protein spartin interacts with Eps15.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SH3 domain binding	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables polyubiquitin modification-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables polyubiquitin modification-dependent protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi to endosome transport	ISO Inferred from Sequence Orthology more info
involved_in Golgi to endosome transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in clathrin coat assembly	ISO Inferred from Sequence Orthology more info
involved_in endocytic recycling	ISO Inferred from Sequence Orthology more info
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endosomal transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of receptor recycling	ISO Inferred from Sequence Orthology more info
involved_in postsynaptic neurotransmitter receptor internalization	ISO Inferred from Sequence Orthology more info
involved_in receptor-mediated endocytosis of virus by host cell	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in symbiont entry into host cell	ISO Inferred from Sequence Orthology more info
involved_in ubiquitin-dependent endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of AP-2 adaptor complex	IDA Inferred from Direct Assay more info	PubMed
located_in aggresome	ISO Inferred from Sequence Orthology more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basal plasma membrane	ISO Inferred from Sequence Orthology more info
located_in ciliary membrane	IDA Inferred from Direct Assay more info	PubMed
part_of clathrin coat of coated pit	IBA Inferred from Biological aspect of Ancestor more info
part_of clathrin coat of coated pit	IDA Inferred from Direct Assay more info	PubMed
located_in clathrin-coated pit	IDA Inferred from Direct Assay more info	PubMed
located_in clathrin-coated vesicle	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in early endosome membrane	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in synapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: epidermal growth factor receptor substrate 15

Names: epidermal growth factor pathway substrate 15; protein AF-1p

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001159964.1 → NP_001153436.1 epidermal growth factor receptor substrate 15 isoform B

See identical proteins and their annotated locations for NP_001153436.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks multiple exons at the 5' end and has a distinct 5' UTR and start codon, compared to variant 1. These differences result in a protein (isoform B) with a shorter and distinct N-terminus, compared to isoform A. Isoform B localizes to early endosome membranes.

Source sequence(s)

AK083176, AW124983, BC048783, CF533628

Consensus CDS

CCDS51259.1

UniProtKB/TrEMBL

A0A0R4J0A0

Related

ENSMUSP00000030281.6, ENSMUST00000030281.12

Conserved Domains (2) summary

pfam07926
Location:89 → 192

TPR_MLP1_2; TPR/MLP1/MLP2-like protein

cl19219
Location:86 → 159

DUF342; Protein of unknown function (DUF342)
NM_007943.3 → NP_031969.1 epidermal growth factor receptor substrate 15 isoform A

See identical proteins and their annotated locations for NP_031969.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (A). Isoform A mainly localizes to clathrin-coated pits at the plasma membrane.

Source sequence(s)

AK083176, AW124983, BB858255, BC048783

Consensus CDS

CCDS18462.1

UniProtKB/Swiss-Prot

P42567, Q8C431

UniProtKB/TrEMBL

Q5JC28, Q80ZL3

Related

ENSMUSP00000099790.4, ENSMUST00000102729.10

Conserved Domains (5) summary

smart00027
Location:121 → 215

EH; Eps15 homology domain

cd00052
Location:228 → 293

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

pfam07926
Location:403 → 506

TPR_MLP1_2; TPR/MLP1/MLP2-like protein

pfam12761
Location:321 → 458

End3; Actin cytoskeleton-regulatory complex protein END3

cl23720
Location:396 → 497

RILP-like; Rab interacting lysosomal protein-like 1 and 2 (Rilpl1 and Rilpl2)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

109137372..109245014

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017319956.2 → XP_017175445.1 epidermal growth factor receptor substrate 15 isoform X5

UniProtKB/TrEMBL

Q5JC28

Related

ENSMUSP00000135034.2, ENSMUST00000176251.8

Conserved Domains (2) summary

smart00027
Location:121 → 215

EH; Eps15 homology domain

TIGR02168
Location:333 → 509

SMC_prok_B; chromosome segregation protein SMC, common bacterial type
XM_006502738.3 → XP_006502801.2 epidermal growth factor receptor substrate 15 isoform X1

UniProtKB/TrEMBL

H3BK65, Q5JC28

Related

ENSMUSP00000135270.2, ENSMUST00000175776.8

Conserved Domains (2) summary

smart00027
Location:121 → 215

EH; Eps15 homology domain

TIGR02168
Location:369 → 545

SMC_prok_B; chromosome segregation protein SMC, common bacterial type
XM_006502740.3 → XP_006502803.2 epidermal growth factor receptor substrate 15 isoform X4

UniProtKB/TrEMBL

Q5JC28

Conserved Domains (2) summary

smart00027
Location:121 → 215

EH; Eps15 homology domain

TIGR02168
Location:369 → 545

SMC_prok_B; chromosome segregation protein SMC, common bacterial type
XM_006502739.5 → XP_006502802.2 epidermal growth factor receptor substrate 15 isoform X2

UniProtKB/TrEMBL

Q5JC28

Conserved Domains (3) summary

smart00027
Location:89 → 183

EH; Eps15 homology domain

cd00052
Location:19 → 71

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

TIGR02168
Location:337 → 513

SMC_prok_B; chromosome segregation protein SMC, common bacterial type
XM_030253192.2 → XP_030109052.1 epidermal growth factor receptor substrate 15 isoform X3

UniProtKB/TrEMBL

Q5JC28

Conserved Domains (3) summary

smart00027
Location:89 → 183

EH; Eps15 homology domain

cd00052
Location:19 → 71

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

TIGR02168
Location:301 → 477

SMC_prok_B; chromosome segregation protein SMC, common bacterial type