RhoGEF2 Rho guanine nucleotide exchange factor 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: RhoGEF2provided by FlyBase
Official Full Name: Rho guanine nucleotide exchange factor 2provided by FlyBase
Primary source: FLYBASE:FBgn0023172
Locus tag: Dmel_CG9635
See related: AllianceGenome:FB:FBgn0023172
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG9635; Dmel\CG9635; dRhoGEF; DRhoGEF; dRhoGEF2; DrhoGEF2; DRhoGEF2; Gef2; l(2)04291; RG2; Rho-GEF2; RhoGEF; rhogef2; rhoGEF2; RhoGef2; RhoGF2; shar; Su(Rho1)2B; T2
Summary: Enables guanyl-nucleotide exchange factor activity. Involved in several processes, including cell elongation involved in imaginal disc-derived wing morphogenesis; gastrulation involving germ band extension; and positive regulation of Rho protein signal transduction. Located in several cellular components, including apical cortex; cortical actin cytoskeleton; and lateral plasma membrane. Is expressed in several structures, including epithelium; gut section; lamina; larval brain; and organism subdivision. Human ortholog(s) of this gene implicated in immunodeficiency 62. Orthologous to several human genes including ARHGEF11 (Rho guanine nucleotide exchange factor 11). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 53E4-53F1; 2-81 cM

Exon count:: 24

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (17027190..17044653)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (12914695..12932158)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Centralspindlin-mediated transport of RhoGEF positions the cleavage plane for cytokinesis.
Title: Centralspindlin-mediated transport of RhoGEF positions the cleavage plane for cytokinesis.
Increased lateral tension is sufficient for epithelial folding in Drosophila.
Title: Increased lateral tension is sufficient for epithelial folding in Drosophila.
Distinct RhoGEFs Activate Apical and Junctional Contractility under Control of G Proteins during Epithelial Morphogenesis.
Title: Distinct RhoGEFs Activate Apical and Junctional Contractility under Control of G Proteins during Epithelial Morphogenesis.
Recruitment of RhoGEF2 to the plasma membrane is sufficient to cause apical constriction and tissue-level responses that are compatible with endogenous invagination processes.
Title: Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis.
RhoGEF2, Pbl, RhoGEF3, and Tum exhibit discrete localization patterns and are required for cell wound repair.
Title: Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair.
Overexpression of DRhoGEF2, and it's functional mammalian ortholog PDZ-RhoGEF (ArhGEF11), at various stages of eye development, resulted in both dPKB/Akt-dependent and -independent phenotypes in Drosophila
Title: The Rho Guanine Nucleotide Exchange Factor DRhoGEF2 Is a Genetic Modifier of the PI3K Pathway in Drosophila.
RhoGEF2 also cooperates with an activated allele of a downstream effector of Ras, Raf (Raf(GOF)).
Title: In Drosophila, RhoGEF2 cooperates with activated Ras in tumorigenesis through a pathway involving Rho1-Rok-Myosin-II and JNK signalling.
early Drosophila embryogenesis is primed to form furrows at either overlapping astral microtubules or the central spindle; the shift to the latter is driven by a corresponding shift from RhoGEF2 to Pebble in controlling furrow formation.
Title: RhoGEF and positioning of rappaport-like furrows in the early Drosophila embryo.
Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2.
Title: The Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2.
Results identify DRhoGEF2 as an upstream regulator of the actomyosin contractile machinery that drives amnioserosa cells pulsations and apical constriction.
Title: DRhoGEF2 regulates cellular tension and cell pulsations in the Amnioserosa during Drosophila dorsal closure.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables G protein-coupled receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables guanyl-nucleotide exchange factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin-mediated cell contraction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in anterior midgut invagination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apical constriction involved in ventral furrow formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell elongation involved in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellularization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ-band extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in morphogenesis of embryonic epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pole cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Rho protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in posterior midgut invagination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pseudocleavage involved in syncytial blastoderm formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actomyosin contractile ring contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of axonogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of embryonic cell shape	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of embryonic cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of myosin II filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to wounding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spiracle morphogenesis, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ventral furrow formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actin cap	IDA Inferred from Direct Assay more info	PubMed
located_in actomyosin contractile ring	IDA Inferred from Direct Assay more info	PubMed
located_in apical cortex	IDA Inferred from Direct Assay more info	PubMed
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basal part of cell	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in lateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: Rho guanine nucleotide exchange factor 2

Names: CG9635-PD; CG9635-PE; CG9635-PF; CG9635-PG; CG9635-PH; RhoGEF2-PD; RhoGEF2-PE; RhoGEF2-PF; RhoGEF2-PG; RhoGEF2-PH; akkordion; guanine nucleotide exchange factor 2; shar pei; transcription unit 2

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

17027190..17044653

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_206146.4 → NP_995868.1 Rho guanine nucleotide exchange factor 2, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995868.1

Status: REVIEWED

UniProtKB/TrEMBL

A1ZAN6, O44113

Conserved Domains (6) summary

cd08756
Location:934 → 1055

RGS_GEF_like; Regulator of G protein signaling (RGS) domain found in the Rho guanine nucleotide exchange factor (RhoGEF) protein

smart00228
Location:260 → 334

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00160
Location:1539 → 1731

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:1770 → 1875

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00130
Location:1151 → 1202

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam10310
Location:2242 → 2378

DUF2413; Protein of unknown function (DUF2413)
NM_001259452.2 → NP_001246381.1 Rho guanine nucleotide exchange factor 2, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246381.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7T7, O44113

Conserved Domains (6) summary

cd08756
Location:934 → 1055

RGS_GEF_like; Regulator of G protein signaling (RGS) domain found in the Rho guanine nucleotide exchange factor (RhoGEF) protein

smart00228
Location:260 → 334

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00160
Location:1537 → 1729

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:1768 → 1873

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00130
Location:1151 → 1202

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam10310
Location:2240 → 2376

DUF2413; Protein of unknown function (DUF2413)
NM_206147.4 → NP_995869.1 Rho guanine nucleotide exchange factor 2, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995869.1

Status: REVIEWED

UniProtKB/TrEMBL

A1ZAN7, O44113

Conserved Domains (6) summary

cd08756
Location:580 → 701

RGS_GEF_like; Regulator of G protein signaling (RGS) domain found in the Rho guanine nucleotide exchange factor (RhoGEF) protein

smart00228
Location:260 → 334

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00160
Location:1185 → 1377

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:1416 → 1521

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00130
Location:797 → 848

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam10310
Location:1888 → 2024

DUF2413; Protein of unknown function (DUF2413)
NM_057969.6 → NP_477317.1 Rho guanine nucleotide exchange factor 2, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477317.1

Status: REVIEWED

UniProtKB/TrEMBL

A1ZAN6, O44113

Related

FBpp0086125

Conserved Domains (6) summary

cd08756
Location:934 → 1055

RGS_GEF_like; Regulator of G protein signaling (RGS) domain found in the Rho guanine nucleotide exchange factor (RhoGEF) protein

smart00228
Location:260 → 334

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00160
Location:1539 → 1731

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:1770 → 1875

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00130
Location:1151 → 1202

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam10310
Location:2242 → 2378

DUF2413; Protein of unknown function (DUF2413)
NM_001299586.1 → NP_001286515.1 Rho guanine nucleotide exchange factor 2, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286515.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4LFF8, O44113

Conserved Domains (6) summary

cd08756
Location:831 → 952

RGS_GEF_like; Regulator of G protein signaling (RGS) domain found in the Rho guanine nucleotide exchange factor (RhoGEF) protein

smart00228
Location:260 → 334

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00160
Location:1436 → 1628

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:1667 → 1772

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00130
Location:1048 → 1099

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam10310
Location:2139 → 2275

DUF2413; Protein of unknown function (DUF2413)