wash washout [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: washprovided by FlyBase
Official Full Name: washoutprovided by FlyBase
Primary source: FLYBASE:FBgn0033692
Locus tag: Dmel_CG13176
See related: AllianceGenome:FB:FBgn0033692
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG13176; Dmel\CG13176; p63; p65; Wash; WASH
Summary: Enables GTPase binding activity; chromatin binding activity; and lamin binding activity. Involved in several processes, including cytoskeleton organization; nuclear envelope budding; and regulation of organelle organization. Located in endosome; nucleus; and perinuclear region of cytoplasm. Part of WASH complex. Is expressed in embryonic/larval hemocyte and organism. Orthologous to several human genes including WASHC1 (WASH complex subunit 1); WASH2P (WASP family homolog 2, pseudogene); and WASH6P (WASP family homolog 6, pseudogene). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 48E6-48E6; 2-66 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (12180493..12182373)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (8067998..8069878)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study shows that Btk29A and WASH are required for luminal clearance and that Btk29A induces WASH phosphorylation propose that the WASH phosphorylation status balances F-actin assembly between the endosomal and cortical F-actin networks to regulate the timing of luminal clearance and airway shape.
Title: WASH phosphorylation balances endosomal versus cortical actin network integrities during epithelial morphogenesis.
Drosophila WASH has conserved functions in integrin receptor recycling and lysosome neutralization.
Title: Drosophila WASH is required for integrin-mediated cell adhesion, cell motility and lysosomal neutralization.
reveal a novel role for Wash in modulating nucleus morphology and in the organization of both chromatin and non-chromatin nuclear sub-structures
Title: Wash interacts with lamin and affects global nuclear organization.
Developmental hemocyte migration requires the interaction of Rho1 with its downstream effector Wash, a Wiskott-Aldrich syndrome family protein.
Title: Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune cell developmental migrations.
Wash, WASp, SCAR, and Whamy are dynamically expressed throughout oogenesis and embryogenesis.
Title: Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
Results establish Wash and Rho as regulators of both linear- and branched-actin networks, and suggest an Arp2/3-mediated mechanism for how cells might coordinately regulate these structures.
Title: Wash functions downstream of Rho and links linear and branched actin nucleation factors.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables GTPase binding	IPI Inferred from Physical Interaction more info	PubMed
enables alpha-tubulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables alpha-tubulin binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables gamma-tubulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables lamin binding	IDA Inferred from Direct Assay more info	PubMed
enables lamin binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Arp2/3 complex-mediated actin nucleation	IBA Inferred from Biological aspect of Ancestor more info
involved_in Arp2/3 complex-mediated actin nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in Arp2/3 complex-mediated actin nucleation	IEA Inferred from Electronic Annotation more info
involved_in actin crosslink formation	IDA Inferred from Direct Assay more info	PubMed
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament bundle assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in endocytic recycling	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule bundle formation	IDA Inferred from Direct Assay more info	PubMed
involved_in nuclear envelope budding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear envelope organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear inner membrane organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell adhesion mediated by integrin	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in prepupal development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of chromosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of endocytic recycling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of lysosomal lumen pH	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of nucleus organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde transport, endosome to Golgi	IBA Inferred from Biological aspect of Ancestor more info
involved_in retrograde transport, endosome to Golgi	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spiracle morphogenesis, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of WASH complex	IBA Inferred from Biological aspect of Ancestor more info
part_of WASH complex	IDA Inferred from Direct Assay more info	PubMed
part_of WASH complex	IEA Inferred from Electronic Annotation more info
part_of WASH complex	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in early endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome	IEA Inferred from Electronic Annotation more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in recycling endosome	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: washout

Names: CG13176-PA; CG13176-PB; wash-PA; wash-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

12180493..12182373

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001299405.1 → NP_001286334.1 washout, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286334.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A0A0B4LF66, Q7JW27

UniProtKB/TrEMBL

L0CR03

Conserved Domains (1) summary

pfam11945
Location:11 → 301

WASH_WAHD; WAHD domain of WASH complex
NM_136895.4 → NP_610739.1 washout, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_610739.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A0A0B4LF66, Q7JW27

UniProtKB/TrEMBL

L0CR03

Related

FBpp0087097

Conserved Domains (1) summary

pfam11945
Location:11 → 301

WASH_WAHD; WAHD domain of WASH complex